32 results about "Betaine-aldehyde dehydrogenase" patented technology

The present invention provides compositions comprising therapeutic nucleic acids such as interfering RNA (e.g., dsRNA such as siRNA) that target aldehyde dehydrogenase (ALDH) gene expression, lipid particles comprising one or more (e.g., a cocktail) of the therapeutic nucleic acids, methods of making the lipid particles, and methods of delivering and / or administering the lipid particles (e.g., for treating alcoholism in humans).

The invention discloses a new betaine aldehyde dehydrogenase 2 gene enabling paddy rice to have the characteristic of light fragrance, and compared with non-fragrant paddy rice nipponbare, the mutation site is not located in coding strand but belongs to the mutation-type betaine aldehyde dehydrogenase 2 gene at the upstream of the coding strand. Three nucleotides are lost at the -81 site at the upstream of the first nucleotide of the initiation codon of the coding strand, and eight nucleotides are inserted into the -1314 site. A new light-fragrance type paddy rice species is cultivated once homozygous progeny is obtained by transferring the gene into non-fragrant paddy rice via hybridization. The invention also discloses a method for screening the paddy rice possessing the light-fragrance type characteristic and a primer of a molecular marker. The new gene is provided for cultivation of the light-fragrance type paddy rice; also the betaine aldehyde dehydrogenase 2 fragrance gene in paddy rice, the primer of molecular marker and the screening method help to perform assistant selection on plants containing light-fragrance gene, are beneficial to improve selection effect, and also help to identify whether the inbred progeny light-fragrance gene formed at the later stage of breeding is in a homozygous state, so that the breeding target can be realized rapidly and effectively.

The invention discloses applications of aldehyde dehydrogenases (ALDH) 1A3 and an encoding gene thereof as a target for preventing and treating invasion and metastasis of colorectal cancer and as an independent prognostic determination factor for the colorectal cancer, and also an application of an expression down-regulation agent, an activity inhibitor and an over-expression effect inhibitor of the ALDH 1A3 in preparation of medicines for reversing the invasion and metastasis of colorectal cancer. The applications have important significance of preventing and treating the invasion and metastasis of colorectal cancer and prognostic determination of the colorectal cancer.

The present invention provides compositions comprising therapeutic nucleic acids such as interfering RNA (e.g., dsRNA such as siRNA) that target aldehyde dehydrogenase (ALDH) gene expression, lipid particles comprising one or more (e.g., a cocktail) of the therapeutic nucleic acids, methods of making the lipid particles, and methods of delivering and / or administering the lipid particles (e.g., for treating alcoholism in humans).

A microorganism capable of producing acrylic acid, comprising a genetic modification that increases activity of CoA acylating aldehyde dehydrogenase (ALDH) catalyzing conversion of 3-hydroxypropionaldehyde (3-HPA) to 3-hydroxy propionyl-CoA (3-HP-CoA) and a genetic modification that increases activity of 3-HP-CoA dehydratase catalyzing conversion of 3-HP-CoA to acrylyl-CoA in the microorganism in comparison with a cell that is not genetically engineered; as well as a method of producing the microorganism, and a method of producing acrylic acid using the same.

The present invention relates to the fields of genetic engineering technology and biocatalysis, and discloses aldehyde dehydrogenases, a gene thereof, construction of a recombinant strain and an application of the recombinant strain in synthesis of furancarboxylic acid. A gene mining technology is used to screen five aldehyde dehydrogenases from comamonas testosteroni SC1588, and amino acid sequences of the aldehyde dehydrogenases are shown in SEQ ID. 1, SEQ ID. 2, SEQ ID. 3, SEQ ID. 4 or SEQ ID. 5. The five aldehyde dehydrogenases can all efficiently catalyze a bio-based furan selective oxidation to synthetize the corresponding furancarboxylic acid. The yield of a target product is up to about 90%, the highest yield can even reach 100%, and a space-time yield is up to 1.9 g.L<-1>.h<-1>. The production process is simple, production conditions are mild and production efficiency is high.

The invention discloses ALDH1A (aldehyde dehydrogenase 1A), an agonist and a catalysate of the ALDH1A, and an application of an inhibitor. Particularly, the invention discloses the application of theALDH1A or the agonist or the catalysate, the ALDH1A is used to prepare a pharmaceutical composition or preparation, and the pharmaceutical composition or preparation is used to treat and / or prevent infantile autism.

The invention discloses a primer and a detection method which can detect a screened molecular marker aiming at aromatic rice with multiple types of aroma genes and amplify a molecular marker of multiple types of betaine aldehyde dehydrogenase genes 2 (Badh2 / badh2) which are directly related to rice aroma formation. The detection method comprises the following steps: (1) extracting total DNAs of the rice as a template, and adding the primer for performing polymerase chain reaction (PCR) amplification; (2) performing restriction endonuclease Alu I digestion on a PCR product; (3) performing gel electrophoresis detection on an enzyme-digested product. The situation that only a mutation type of the aroma allele can be identified in the process of detecting a molecular marker of aroma genes in the past is broken, plants containing different types of the aroma genes can be selected in an assisted mode, a mutation type of the aroma allele of a parent aromatic rice variety is not required to determined before the operation of performing novel aromatic rice variety breeding on the molecular marker in an assisted mode, and pre-operation steps of molecular marker-assisted breeding are simplified, so that a breeding objective for breeding a novel aromatic rice variety is rapidly and effectively realized.

The present invention relates to rice breeding gene technology in biotechnology field, and is rice aroma gene of paddy rice and the constructing process and use of expression vector including the rice aroma gene. The rice aroma gene of paddy rice has nucleotide of total length of 9066 bases and including 1242 bases in start codon ATG upstream, 5859 bases in the gene region and 1965 bases in the termination codon TAA downstream, coding area comprising 15 exons, and gene sequence as changed betaine aldehyde dehydrogenase coding gene Badh with an 8 base deletion in the 7-th exon. Through cutting the integral rice aroma gene of paddy rice of the positive BAC clone with the limiting cleavage sites HindIII on two sides of the rice aroma gene of paddy rice Badh and cloning to expression vector pCAMBIA1302, the expression vector of rice aroma gene of paddy rice may be obtained and may be used in breeding excellent rice variety.

The invention relates to a method for designing gene sequences, in particular to a human aldehyde dehydrogenase 2 sequence designed according to a preferred codon of pichia pastoris. The sequence is characterized in that a DNA molecule is designed according to the preference of the pichia pastoris, and a code of the molecule has the same nucleotide sequence as a protein coded by a human aldehyde dehydrogenase 2 gene ID 217 on an amino acid level. The human aldehyde dehydrogenase 2 gene sequence designed according to the preferred codon of pichia pastoris is realized by the following steps: on the basis of a human ALDH2 gene (gene ID 217), UTR and signal peptide are deleted; enzyme cutting sites of an incision enzyme EcoR I and an incision enzyme Not I are added; a codon GCG for coding Ala is replaced by GCU according to the use method of the pichia pastoris codon, and codons CGC and CGG for coding Arg are replaced by AGA, AGG, CGU and CGA; and altogether 28 bases are modified to obtain a target sequence ALDH2. The gene sequence can express with high efficiency in the pichia pastoris, can be applied to preventing alcoholism and protecting livers and has the effects of preventing alcoholism and protecting livers.

The invention relates to thermophilic long-chain alkyl aldehyde dehydrogenase in an n-alkane end degradation process of thermophilic denitrified bacillus and a crystal structure thereof. A molecular clone method is utilized, so that a target gene is cloned into escherichia coli (E. coli) so as to be heterologously expressed. A purified product of aldehyde dehydrogenase is obtained through a protein purification method. A crystal of the aldehyde dehydrogenase, which is suitable for diffraction, is obtained through a hanging droplet crystallization method. The crystal structure of the aldehyde dehydrogenase is analyzed by utilizing an X-ray diffraction method. The results show that the aldehyde dehydrogenase contains a characteristic ALDH fold which is formed by three structural domains. Site-specific mutagenesis is carried out on conserved residues on an enzyme activity part, and the influence of the conserved residues on enzyme activity is proved through wild type of the enzyme and enzyme activity experiment of the mutant thereof. The results can comprehensively reflect space conformation of the enzyme, and provide theoretical guidance for further researching relation between structures and functions of the aldehyde dehydrogenase, and improving the degradation activity of the enzyme.

The invention discloses new genes for encoding clonorchis sinensis type I aldehyde dehydrogenase, polypeptides encoded by the genes, and antibody of the polypeptides. The invention also discloses the depressor of the polypeptides for screening clonorchis sinensis type I aldehyde dehydrogenase, the use of polynucleotides as primer or as probe, in particular the use of the polypeptide and polynucleotide as diagnosis reagent kit, and vaccine.

The invention discloses an application of acetaldehyde dehydrogenase 2 as a drug target for treating tumor cells with anthracycline type chemotherapy drugs. Drug screening shows that the anthracycline type chemotherapy drugs can specifically kill renal clear cell cancer cells with VHL deletion or mutation and has small toxicity to normal cells, thus providing an effective choice for chemotherapy of renal cancer and overcoming the disadvantage that the renal cancer is only treated through operations in clinical at present. Further study shows the sensitivity of the acetaldehyde dehydrogenase 2 mediated anthracycline type chemotherapy drugs to tumor cells, thus remaindering the application of the acetaldehyde dehydrogenase 2 as the drug target for treating tumor cells with the anthracycline type chemotherapy drugs. Deletion or mutation of the acetaldehyde dehydrogenase 2 exists in 40% of populations in Asia, so that the application of the acetaldehyde dehydrogenase 2 has a broader application prospect. For patients without the deletion or the mutation of the acetaldehyde dehydrogenase 2, killing effects on tumor cells can be significantly enhanced through combination of the anthracycline type chemotherapy drugs and an inhibitor of the acetaldehyde dehydrogenase 2.

A betaine aldehyde dehydrogenase (BADH) derived from bruguiear gymnorrhiza, coding gene thereof, and application of the coding gene in cultivating improved salt-tolerant and drought-tolerant transgenic plants.

The present invention discloses a new betaine aldehyde dehydrogenase 2 gene that can make rice show a light-flavored characteristic. Compared with the non-perfume rice "Nipponbare", the mutation site is not located on the coding chain, but belongs to the upstream mutation of the coding chain. Types of betaine aldehyde dehydrogenase 2 genes. There was a deletion of 3 nucleotides at position -81 upstream of the first nucleotide of the initiation codon of the coding strand, and an insertion of 8 nucleotides at -1314. By crossing this gene into non-perfume rice and obtaining homozygous offspring, a new light-scented rice variety can be bred. The invention also discloses a method for screening rice with the light-scented characteristic gene and molecular marker primers. The invention provides new genes for cultivating light-scented rice, and can also assist in selecting plants containing light-scented genes, which is conducive to improving the selection effect, and can also determine whether the light-scented genes of self-bred offspring formed in the later stage of the breeding process are in a homozygous state Identification to achieve breeding goals quickly and efficiently.