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Method for preparing low-citrinin monascus pigment through submerged fermentation

A technique of monascus red pigment and submerged fermentation, which is applied in the field of preparation of submerged fermentation low citrinin monascus red pigment, can solve the problems of citrinin carcinogenicity, genotoxicity, teratogenicity and the like, and achieves the effect of high color value

Inactive Publication Date: 2010-06-16
东莞市天益生物工程有限公司
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AI Technical Summary

Problems solved by technology

However, in the process of preparing monascus pigment, a biological toxin, citrinin, is usually produced
Citrinin is carcinogenic, teratogenic, and genotoxic, which has a serious negative impact on the promotion of red yeast rice products to the world

Method used

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Embodiment Construction

[0011] Below is the specific embodiment of the present invention

[0012] The present invention obtains the citrinin-free monascus red product produced industrially through gene knockout metabolic engineering, traditional process optimization technology, membrane separation technology, etc., and its preparation method comprises:

[0013] 1, bacterial classification adopts Monascus T1166 bacterial strain; The selection and breeding of excellent strain of Monascus, the Monascus that the present invention adopts is TII66 bacterial strain, and this is that Dongguan Tianyi Biological Engineering Co., Ltd. provides.

[0014] 2. Culture medium, the culture medium for bacteria can use the following medium.

[0015] (1) separation medium PDA, its composition is: 20% potato 1000ml, glucose 20g, agar 20g;

[0016] (2) Separation medium CYA, its composition is: sucrose 3%, yeast extract 5.0%, KH 2 PO 4 0.1%, NaNO 3 0.3%, MgSO 4 ·7H 2 O0.05%, FeSO 4 ·7H 2 O0.001%, distilled water 1...

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Abstract

The invention relates to the technical field of biological fermentation methods, in particular to a method for preparing low-citrinin monascus pigment through submerged fermentation. The preparation method comprises the following steps that: A, taking monascus rubber 6611 bacterial strain as a strain; B, ensuring that a strain medium is that: (1) an isolation medium PDA; (2) an isolation medium CYA; (3) a slant medium KMA; C, breeding the strain through a genetic engineering technique, ultraviolet mutation or chemical mutagenesis; D, fermenting the medium, namely taking rice as main raw materials (carbon source), taking soybean, peptone, corn steep liquor and the like as auxiliary materials (nitrogen source), and adjusting a pH value through lactic acid; E, carrying out fermentation, namely using a liquid fermentation tank to ferment, wherein the temperature of fermentation is 32 to 36 DEG C; the ventilation ratio is 1:0.3-0.6; the pressure of the tank is 0.4 to 0.7 MPa; the time is 65 to 85H; and F, the monascus pigment is extracted. The monascus pigment prepared by the method has high color standard, while the content of the citrinin is very low, even no citrinin is contained.

Description

Technical field: [0001] The invention relates to the technical field of biological fermentation methods, in particular to a method for preparing monascus red pigment with low citrinin in submerged fermentation. Background technique: [0002] Monascus genus Monascus purple, also known as Monascus, is cultivated and fermented in rice with Monascus. Traditionally, red yeast rice is widely used in food fields such as rice wine and vinegar. According to modern medical research reports, red yeast rice has the effect of lowering blood pressure and blood fat, and the monascus K contained in it can prevent the formation of cholesterol. Monascus pigment is also a traditional product in my country, and it is one of the most successful natural pigments produced by microbial fermentation and widely used. However, in the process of preparing monascus pigment, a kind of biotoxin-citrinin is usually produced. Citrinin is carcinogenic, teratogenic, and genotoxic, which has a serious negat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02C12N15/01C12R1/66
Inventor 杨晓暾胡文林谢凤娇陈影妮胡文贵张婉瑶刘建国彭振球
Owner 东莞市天益生物工程有限公司