Raw material formula of hydrophilic interaction electrochromatographic monolithic column and method for preparing same

A technology of hydrophilic interaction and monolithic column, applied in the field of electrochromatography, can solve the problems such as not obtaining good results, and achieve the effects of low cost, simple preparation method and good precision

Inactive Publication Date: 2010-07-07
苏州环球色谱有限责任公司
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Problems solved by technology

At present, the analysis and detection methods of melamine mainly include gas chromatography-mass spectrometry (GC-MS), liquid chromatography-mass spectrometry (LC-MS), high performance liquid chromatography (HPLC), enzyme-linked immunosorbent method and other methods, but due to the It is a small molecular basic compound with strong polarity, and none of the above analysis and detection methods have achieved good results

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  • Raw material formula of hydrophilic interaction electrochromatographic monolithic column and method for preparing same
  • Raw material formula of hydrophilic interaction electrochromatographic monolithic column and method for preparing same
  • Raw material formula of hydrophilic interaction electrochromatographic monolithic column and method for preparing same

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Embodiment 1

[0030] A method for preparing a monolithic column of hydrophilic interaction electrochromatography, comprising the steps of:

[0031] (1) Pretreatment of the capillary: wash with 1mol / L HCl for 30min, then wash with deionized water until neutral, wash with 0.1mol / LNaOH for 3h, then wash with deionized water until neutral, then rinse with acetone for 15min, nitrogen Blow dry for later use; add a mixture of acetone and methacryloxypropyl trimethoxysilane in a volume ratio of 1:1 to the dried capillary, react in a water bath at 60°C for 24 hours, rinse with acetone for 30 minutes after the reaction, and use Blow dry with nitrogen for later use;

[0032] (2) Preparation of capillary monolithic column: mix the obtained mixture according to the raw material formula, after ultrasonic mixing for 10 minutes, pass nitrogen to remove the air in the mixture, then inject the mixture into the capillary obtained in step (1), seal both ends of the capillary, immerse React in a water bath at ...

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Abstract

The invention discloses a raw material formula of a hydrophilic interaction electrochromatographic monolithic column and a method for preparing the same by the raw material formula. The raw material formula comprises the following components by weight percent: 12-25% of neutral compound monomer, 10-18% of cross-linking agent, 2-15% of hydrophilic compound monomer, 50-65% of pore-forming agent and 0.1-0.4% of initiator, wherein the hydrophilic compound monomer is 3-[N,N-dimethyl-[2-(2-methylprop-2-enoyloxy) ethyl-butyl ammonium]propane-1-sulfonate or 2-acrylamido-2-methylpropane sulfonic acid (AMPS). The electrochromatographic monolithic column can analyze and detect the small molecule polar compound such as melamine. The test shows that the detection limit uses signal-to-noise ratio 3 as indicator, the detection limit of the monolithic column is 0.05mg/L, the quantitation limit uses signal-to-noise ratio 10 as indicator, the quantitation limit is 0.1mg/L, and the accuracy is good.

Description

technical field [0001] The invention belongs to the technical field of electrochromatography, and in particular relates to a raw material formula and a preparation method of a monolithic column of hydrophilic interaction electrochromatography. Background technique [0002] Capillary Electrochromatography (CEC) is an efficient and rapid micro-separation technique that has developed rapidly in recent years. CEC is a chromatographic stationary phase that is filled in the capillary or coated on the capillary wall, bonded to the chromatographic stationary phase, or formed by cross-linking and copolymerization, driven by electroosmotic flow (EOF), so that the compound molecules with different charged properties are fixed according to their A chromatographic mode in which separation and analysis are achieved by the difference in adsorption between the phase and the mobile phase, the difference in the equilibrium distribution constant, and the difference in the electrophoretic rate....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/288B01J20/30
CPCB01D15/305
Inventor 闫超李新燕
Owner 苏州环球色谱有限责任公司
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