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Method for immobilizing microorganisms and repairing defects of cement-based materials by adopting sodium carboxymethyl cellulose

A technology of sodium carboxymethyl cellulose and microbial repair, applied in the field of surface defect repair and protection of cement-based materials, can solve problems such as poor compatibility of substrates, and achieve good weather resistance, significant protective effect of film coating, and tight bonding. Effect

Inactive Publication Date: 2010-07-07
SOUTHEAST UNIV
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Problems solved by technology

The prior art usually utilizes organic polymers, such as epoxy resins, acrylic resins, especially silicone or organic fluorine polymers to protect and repair the surface defects of cement-based materials, and has achieved good repair effects, but these organic polymer materials The problems of long-term weather resistance and poor compatibility with substrates are becoming the research direction of researchers

Method used

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  • Method for immobilizing microorganisms and repairing defects of cement-based materials by adopting sodium carboxymethyl cellulose
  • Method for immobilizing microorganisms and repairing defects of cement-based materials by adopting sodium carboxymethyl cellulose
  • Method for immobilizing microorganisms and repairing defects of cement-based materials by adopting sodium carboxymethyl cellulose

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Embodiment 1

[0038] Inoculate the strain Bacillus pasteurii on the medium containing the substrate containing urea, and carry out shaking culture at 25-37°C. After 16-24 hours, when the concentration of the strain reaches 5×10 8 When cell / mL, the bacterial liquid was centrifuged at 5000-8000rpm for 5-8min to obtain a highly concentrated bacterial liquid concentration of 9.8×10 10 cell / mL, spread on the surface of the sample, then add 2% mass fraction sodium carboxymethyl cellulose solution, and 1.0mol / L urea and Ca(NO 3 ) 2 The mixed solutions were applied successively on the surface of the specimens for the coating experiment. After 7 days, there were obvious continuous white deposition adhesion layers on the surface of the cement stone specimens (such as figure 2As shown), it is closely combined with the surface of cement stone, and the sample is confirmed to be calcite by XRD analysis, as shown in image 3 shown. Since carboxymethyl cellulose sodium is used to immobilize bacterial s...

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Abstract

The invention discloses a method for immobilizing microorganisms and repairing the defects of cement-based materials by adopting sodium carboxymethyl cellulose, in particular to a method for filming protection by utilizing the enzymatic conversion action of microorganisms, adhering strains to the surface of cement stone to grow, and mineralizing the surface of the cement stone to deposite a calcium carbonate layer. By adopting the method, a continuous, compact and hard calcium carbonate protection layer is generated and tightly combined with the surface of a substrate. Due to the tight adhesion of the continuous and compact calcium carbonate layer on the surface, the capillary water-absorbing coefficient of a cement stone test piece is obviously decreased from original 1688g / (m2*hl / 2) to 298.6g / (m2*hl / 2) after covering a film so that the amplitude is reduced by over 80%, and the protection effect is remarkable. The invention has rich resources, clean environment, low cost and simple process.

Description

technical field [0001] The present invention relates to a method for repairing and protecting surface defects of cement-based materials, in particular to using a soil bacterium with carbonate mineralization characteristics to mineralize and deposit continuous and dense particles on the surface of cement-based materials through the enzymatic action in its life process. The calcium carbonate layer is a method for repairing and protecting surface defects of cement-based materials. Background technique [0002] For cement-based materials exposed to nature, due to the influence of physical, chemical and biological factors for a long time, the properties of the matrix are gradually destroyed, and the surface is often loose, micro-cracks and other phenomena, resulting in an increase in its internal porosity. The mechanical properties continue to decline. Moreover, with the rapid development of modern industry, the erosion of cement-based materials caused by environmental pollution...

Claims

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Application Information

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IPC IPC(8): C04B41/50C04B41/65
Inventor 钱春香王瑞兴王剑云
Owner SOUTHEAST UNIV
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