Method for preparing phosphoryl oligosaccharide by holoenzyme method

A technology for the preparation of oligophosphate oligosaccharides and enzymatic method, which is applied in fermentation and other fields, can solve the problems of detailed research on the production of oligophosphate oligosaccharides, and achieve the effects of less energy consumption, greater economic benefits, and high processing efficiency

Inactive Publication Date: 2010-08-04
ANHUI AGRICULTURAL UNIVERSITY
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Problems solved by technology

In the past ten years, domestic research and reports on oligophosphate oligosaccharides have been very few. There are only simple reports on oligo

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  • Method for preparing phosphoryl oligosaccharide by holoenzyme method
  • Method for preparing phosphoryl oligosaccharide by holoenzyme method
  • Method for preparing phosphoryl oligosaccharide by holoenzyme method

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Embodiment Construction

[0019] 1) Blending. First pour water into the batching tank, then slowly add 1 ton of raw starch under constant stirring, and use a Bomei meter for online detection until the slurry concentration is 10 o Be, then add 0.65kg CaCl 2 As an enzyme activity accelerator, use HCl and Na 2 CO 3 Adjust the slurry to a pH of 5.4.

[0020] 2) Liquefaction. After pumping the material into the jet liquefier, add a new type of high-temperature-resistant α-amylase to it, with an amount of 2,100,000u / ton of starch. In the jet, the slurry and the steam meet directly and fully, and the jet temperature is 110°C, and maintained for 4 ~8min, control the discharge temperature at 95~97°C, spray the liquefied feed liquid into the laminar flow tank, keep it at 95°C for 30min, and when the typical test reaction shows the true color of iodine, pass steam to kill the enzyme. The DE value of the liquefied material is 15% to 17%.

[0021] 3) Primary plate and frame filter press. When pressing the ...

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Abstract

The invention discloses a method for preparing functional oligosaccharide, i.e. phosphoryl oligosaccharide by a holoenzyme method, which takes potato starch as the raw material. The invention adopts a novel high temperature resistant alpha-amylase to combine with the low-pressure steam injection and liquefaction technology to liquefy the potato starch; CaCl2 is added to serve as an erzyme activity accelerator; after the mixture is fully liquefied, liquefied liquid is saccharified by fungal enzyme after plate-frame pressure filtration; pullulanase is added for synergy to ensure that starch conversion rate is more than or equal to 98%; and malt oligosaccharide syrup with low DE value is prepared; 001*7 strong acidic styrene cation exchange resin is used for processing the syrup to remove parts of impurities with positive charges and coluring matters; then, 7170 strong-basicity styrene anion exchange resin is adopted for processing to remove natural sugar to obtain the phosphoryl oligosaccharide with negative charges; and finally, sugar liquor obtained by separation is concentrated and dried to obtain the target product.

Description

1. Technical field [0001] The invention relates to a preparation method of oligosaccharides, specifically a method for preparing a novel functional oligosaccharide-phosphate oligosaccharide by using potato starch as a raw material and adopting an all-enzyme method. 2. Technical Background [0002] Oligosaccharides, also known as oligosaccharides, refer to linear or branched low-level polymers formed by connecting 2 to 10 monosaccharides through glycosidic bonds. According to the biological functions of oligosaccharides, they can be divided into two categories: functional oligosaccharides and ordinary oligosaccharides. Functional oligosaccharides refer to those with special physiological functions, especially those that are not absorbed by the intestinal tract of humans and animals and promote biologic function. Proliferation of fidobacteria, a class of oligosaccharides beneficial to gut health, the bifidofactors. [0003] The concept of phosphate oligosaccharides (Phosphory...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/00
Inventor 杜先锋杨文军刘霞
Owner ANHUI AGRICULTURAL UNIVERSITY
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