Unlock instant, AI-driven research and patent intelligence for your innovation.

L-cysteine/chitosan-modified electrochemical sensor and use thereof

A cysteine ​​and chitosan technology, applied in the fields of electrochemical variables of materials, scientific instruments, instruments, etc., can solve the problems of expensive instruments and low selectivity of fluorescence methods, and achieve improved detection sensitivity and strong anti-interference ability. , the effect of low cost

Inactive Publication Date: 2012-10-31
UNIV OF JINAN
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the common methods for detecting copper ions include fluorescence method, atomic absorption spectrometry, colorimetry, ion chromatography, etc., but they have some defects, such as the low selectivity of the fluorescence method, and the atomic absorption spectrometry instrument is expensive and requires a full-time professional personnel to operate

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • L-cysteine/chitosan-modified electrochemical sensor and use thereof
  • L-cysteine/chitosan-modified electrochemical sensor and use thereof
  • L-cysteine/chitosan-modified electrochemical sensor and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The electrochemical sensor modified by L-cysteine / chitosan of the present invention mainly consists of glassy carbon electrodes, such as figure 1 The glassy carbon electrode shown is mainly composed of a glassy carbon substrate 1 , an electrode lead 3 connected to the glassy carbon substrate, and an insulating layer 4 . The surface of the glassy carbon substrate 3 is adsorbed with an L-cysteine / chitosan composite film 2 .

[0027] The preparation method of this electrochemical sensor is:

[0028] (1) Grinding the glassy carbon electrode with polishing powder to make the base surface smooth and clean, and then cleaning the surface with ultrasonic waves at a frequency of 53KHZ and a temperature of 25°C to obtain a pretreated glassy carbon electrode;

[0029] (2) adding 1.0g chitosan and 0.6gL-cysteine ​​to 200ml volume fraction is 1% acetic acid solution, stirring makes them fully dissolve;

[0030] (3) Immerse the pretreated glassy carbon electrode in the solution prep...

Embodiment 2

[0035] L-cysteine / chitosan modified electrochemical sensors include glassy carbon electrodes, such as figure 1 The glassy carbon electrode shown is mainly composed of a glassy carbon substrate 1 , an electrode lead 3 connected to the glassy carbon substrate, and an insulating layer 4 . The surface of the glassy carbon substrate 3 is adsorbed with an L-cysteine / chitosan composite film 2 .

[0036] The preparation method of this electrochemical sensor is:

[0037] (1) Grinding the glassy carbon electrode with polishing powder to make the base surface smooth and clean, and then cleaning the surface with ultrasonic waves at a frequency of 53KHZ and a temperature of 25°C to obtain a pretreated glassy carbon electrode;

[0038] (2) 1.4g chitosan and 1.0gL-cysteine ​​are added to 200ml volume fraction in the acetic acid solution of 2%, stirring makes them fully dissolve;

[0039] (3) Immerse the pretreated glassy carbon electrode in the solution prepared in step (2), and let it sta...

Embodiment 3

[0043] L-cysteine / chitosan modified electrochemical sensors are mainly composed of glassy carbon electrodes, such as figure 1 The glassy carbon electrode shown is mainly composed of a glassy carbon substrate 1 , an electrode lead 3 connected to the glassy carbon substrate, and an insulating layer 4 . The surface of the glassy carbon substrate 3 is adsorbed with an L-cysteine / chitosan composite film 2 .

[0044] The preparation method of this electrochemical sensor is:

[0045] (1) Grinding the glassy carbon electrode with polishing powder to make the base surface smooth and clean, and then cleaning the surface with ultrasonic waves at a frequency of 53KHZ and a temperature of 25°C to obtain a pretreated glassy carbon electrode;

[0046] (2) adding 1.2g chitosan and 0.8gL-cysteine ​​to 200ml volume fraction is 1% acetic acid solution, stirring makes them fully dissolve;

[0047] (3) Immerse the pretreated glassy carbon electrode in the solution prepared in step (2), and let i...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
viscosityaaaaaaaaaa
Login to View More

Abstract

The invention discloses an L-cysteine / chitosan-modified electrochemical sensor, which mainly comprises a glassy carbon electrode, wherein an L-cysteine / chitosan composite film is coated on the surface of the glassy carbon electrode. The invention also discloses a preparation method, which comprises: pretreating the glassy carbon electrode; preparing the acetic acid solution of the chitosan and L-cysteine; immersing the pretreated glassy carbon electrode in the prepared solution, and standing the solution at room temperature for 20 to 40 minutes to obtain the L-cysteine / chitosan composite film; and washing the glassy carbon electrode to remove the residual L-cysteine and chitosan to obtain the L-cysteine / chitosan-modified electrochemical sensor. The electrochemical sensor of the invention has the characteristics of high speed, high sensitivity and high anti-interference performance, and when used in copper ion detection, has high anti-interference performance and a detection limit up to 2.0x10<-11>mol / L.

Description

technical field [0001] The invention relates to an electrochemical sensor modified by L-cysteine / chitosan and application thereof. Background technique [0002] Copper is one of the essential trace elements for living organisms, and plays an important role in regulating the metabolism of the body. In organisms, copper exists in the form of copper protein bound to protein, which is an important component of many oxidative enzymes. The lack of copper will affect the endocrine function and the physiological activity of some enzymes, but the excessive intake of copper will cause harm. Studies have shown that the content of copper in the blood is significantly correlated with diseases of the cerebrovascular system and liver. Therefore, it is of great practical significance to study sensitive, rapid and simple analytical methods for trace copper. At present, the common methods for detecting copper ions include fluorescence method, atomic absorption spectrometry, colorimetry, io...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/413G01N27/28G01N27/38
Inventor 庞雪辉魏琴谭福能隋卫平张洁
Owner UNIV OF JINAN