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Active component extracted by using Bacillus licheniformis and application thereof

A technology of Bacillus licheniformis, active ingredients, applied in the direction of methods, applications, bacteria, etc. using spores

Inactive Publication Date: 2010-08-18
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, literature search has not found any public reports that the metabolites in Bacillus licheniformis can induce fungi to form chlamydospores

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The strain of B. licheniformis N27 was inoculated on the slant of the solid medium of the test tube, and cultured at 28° C. for 4 days to obtain the strain of the test tube. The test tube was inoculated into a 500ml Erlenmeyer flask (100ml of liquid medium in each bottle), and cultured on a shaker at 25°C (150rpm) for 5 days. Concentrate the fermented liquid containing the bacteria to one-tenth of the original volume under reduced pressure at 45°C, extract with n-butanol three times, and concentrate the extracted part of n-butanol to dryness under reduced pressure at 45°C to obtain a crude extract . Put the crude extract on the silica gel H filler, first elute it with a mixed solvent of ethyl acetate and methanol with a volume ratio of 1:1, then wash it all down with methanol, and concentrate the washed part of methanol under reduced pressure at 45°C to obtain X fraction. Fraction X was separated with C18 packing, and eluted with 50% and 100% methanol: water successiv...

Embodiment 2

[0023] The strain of B. licheniformis N27 was inoculated on the slant of the solid medium of the test tube, and cultured at 28° C. for 4 days to obtain the strain of the test tube. The test tube was inoculated into a 500ml Erlenmeyer flask (200ml of liquid medium in each bottle), and cultured on a shaker at 28°C (rotating speed: 200rpm) for 3 days. Concentrate the fermented liquid containing the bacteria to one-tenth of the original volume under reduced pressure at 45°C, extract with n-butanol three times, and concentrate the extracted part of n-butanol to dryness under reduced pressure at 45°C to obtain a crude extract . Put the crude extract on a silica gel H column, elute it first with a mixed solvent of ethyl acetate and methanol with a volume ratio of 3:1, then wash it down with methanol, and concentrate the washed part of methanol under reduced pressure at 45°C to obtain X fraction. Fraction X was separated with C18 packing and eluted sequentially with 70% and 100% met...

Embodiment 3

[0025] The strain of B. licheniformis N27 was inoculated on the slant of the solid medium of the test tube, and cultured at 28°C for 4 days to obtain the strain of the test tube. The test tube seeds were inoculated into 500ml Erlenmeyer flasks (350ml per bottle), and cultured on a shaker at 32°C (rotating speed: 250rpm) for 2 days. Concentrate the fermented liquid containing the bacteria to one-tenth of the original volume under reduced pressure at 45°C, extract with n-butanol three times, and concentrate the extracted part of n-butanol to dryness under reduced pressure at 45°C to obtain a crude extract . Put the crude extract on a silica gel H column, first elute it with a mixed solvent of ethyl acetate and methanol with a volume ratio of 4:1, then wash it all down with methanol, and concentrate the washed part of methanol under reduced pressure at 45°C to obtain X fraction. Fraction X was separated with C18 packing and eluted with 80%, 100% methanol: water in sequence. The...

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Abstract

The invention relates to an active component extracted by using Bacillus licheniformis and application thereof, belonging to the technical field of biological pesticides. The active component is extracted by the following steps of: taking Bacillus licheniformis N27 with the preservation number of CGMCC No:3585 as a producing strain; fermenting the B. licheniformis N27 with liquid, wherein a culture medium contains 200g of potatoes, 20 g of glucose and 1000ml of water, and the fermenting conditions are that the temperature is 25-32 DEG C and the rotate speed is 150-250 rpm, and culturing for 2-5 days by a shaker; decompressing and condensing the obtained fermentation liquor to obtain crude extract; and separating the crude extract sequentially with silica gel H, C18 and SephadexLH-20 to obtain the active component Z. The active component is a metabolite which ensures that fungus cultured in a conventional culture medium can effectively and fast form chlamydospore, has low use level and is applied in the preparation of biological pesticides.

Description

Technical field: [0001] The invention relates to an active ingredient extracted by bacillus licheniformis and its application, and belongs to the technical field of biological pesticides. Background technique: [0002] As more and more toxic chemical pesticides are gradually restricted in use, biopesticides have become the focus of the development of the global pesticide industry due to their wide source of raw materials, safety to non-target organisms, low toxicity and side effects, and good environmental compatibility. new trend. Many genera or strains of fungi are used to control pests: such as Trichoderma, Fusarium oxysporum, Pythium oligandrum, Verticillium chlamydosporium, etc. In the development of biopesticides using fungi, it is very important to produce chlamydospores, because they are rich in endogenous nutrients, and their stress resistance is much higher than that of conidia and mycelia, which can overcome the short survival period of conidia preparations. The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N3/00A01N63/04C12R1/10
Inventor 李蕾周敬伟张克勤
Owner YUNNAN UNIV
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