Process for extracting superoxide dismutase (SOD) from houttuynia, hovenia dulcis thumb, rosa roxburghii, myrobalan, bottle gourd and chayote

Abstract

The invention discloses a process for extracting superoxide dismutase (SOD) from houttuynia, hovenia dulcis thumb, rosa roxburghii, myrobalan, bottle gourd and chayote, which comprises the following steps of: A) preprocessing raw materials, homogenizing the materials by using a homogenizer and centrifuging at the high speed of 8,000 to 10,000 rpm and filtering to produce crude extract; B) allowing the obtained crude extract to pass through polyethylene glycol (PEG), glutathione S-transferase (GST) and nickel (Ni) affinity chromatographic column purified target protein sequentially; C) adjusting the pH value of the crude extract obtained by the step B) to be 7 to 8; D) performing ultrafiltration on the eluent obtained by the step C) by using an ultrafiltration membrane to prepare high-purity SOD; and E) further concentrating the ultrafiltrate to produce SOD concentrated solution and performing freeze drying on the SOD concentrated solution to produce freeze-dried powder for later use. Due to the extracting process, the purity of the separated and purified high-purity SOD can reach 98 percent; the enzyme activity can reach 5.6*10<6>U / g; and the process has high economic value.

Description

technical field

[0001] The invention belongs to the technical field of biological enzyme separation and purification engineering, and in particular relates to the extraction of high-purity superoxide dismutase (SOD) from plants such as Houttuynia cordata, Hovenia dulcis, Rosa roxburghii, citronella, gourd, and chayote technology. Background technique

[0002] Superoxide dismutase (SOD), as an important scavenger of oxygen free radicals in organisms, can balance oxygen free radicals in the body and avoid adverse reactions caused by excessive concentrations of superoxide anion free radicals in organisms. In radiation protection, diabetes, lung disease, hepatitis, cardiovascular disease, tumor, lupus erythematosus, cataract, rheumatoid, arthritis, degenerative inflammation, exudative inflammation, proliferative inflammation, atopic inflammation and autoimmune therapy, etc. Unique efficacy. At the same time, superoxide dismutase can be used as an active ingredient in beauty co...