Tetraploid locust cuttage breeding method
A tetraploid Robinia pseudoacacia cutting propagation technology, applied in the field of garden plants, can solve the problems of not being easy to obtain root segments, affecting sustainable seedling cultivation, and complicated root digging operations, so as to increase the rooting rate, promote the formation of callus, The effect of increasing the number of germination
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Embodiment 1
[0064] 1. Acquisition of Robinia pseudoacacia hard branches and treatment with growth regulator for the first time
[0065] 1) At the end of October of that year, after the tetraploid Robinia pseudoacacia stopped growing and had fallen leaves, cut the annual robust and pest-free branches, and trim and remove the side branches of the branches. into 10-20cm branches, and the branches with a diameter of 10-15mm are trimmed into 20-30cm branches, and the lower end of the branch is trimmed into a horseshoe-shaped oblique section, the upper end is a flat section, and the upper and lower incisions are 0.5cm away from the buds. The diameter of the segment is 10-20mm;
[0066] 2) Bind every 30 pruned cuttings into a bundle to form a bundle of branches, wherein the lower end of the branches in the bundle is strictly separated from the upper end, that is, the lower ends of all branches are located at one end of the bundle, and all branches The upper end of the branch is located at the o...
Embodiment 2
[0094] Except that in the germination accelerating step of burying the branches with sand, the branches are covered with 4cm thick wet coarse sand, all the others are the same as in Example 1.
[0095] After 15 days of cutting, the seedlings were hardened mainly by taking measures such as ventilation, reducing the amount of water spray, and increasing the intensity of light. At the same time, the rooting length and number of roots of the cuttings were measured, and the rooting rate was calculated. The test results are shown in Table 1.
Embodiment 3
[0097] In addition to using the IBA solution with a concentration of 100mg / kg to soak the branches in the first growth regulator treatment process, the soaking time is 3min; in the germination step of the sand-buried branches, the branches are covered with 4cm thick wet coarse sand; In the step of treating cuttings in the cutting treatment process, 1500mg / kg of IBA solution was used to treat the base of the cuttings, except that the dipping time was 20s, and the rest were the same as in Example 1.
[0098] After 15 days of cutting, the seedlings were hardened mainly by taking measures such as ventilation, reducing the amount of water spray, and increasing the intensity of light. At the same time, the rooting length and number of roots of the cuttings were measured, and the rooting rate was calculated. The test results are shown in Table 1.
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