Chlorella and application thereof

A technology of chlorella and microalgae oil, which is applied in the direction of single-cell algae, biochemical equipment and methods, microorganisms, etc., can solve the problem of high production cost of microalgae oil, and achieve the effect of reducing the cost of cultivation and high immobilization efficiency

Inactive Publication Date: 2010-09-08
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The technical problem to be solved by the present invention is to provide a kind of chlorella, which can be used to efficiently capture CO in the gas while producing high-yield oil. 2 , to solve the problem of high production cost of existing microalgae oil

Method used

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  • Chlorella and application thereof
  • Chlorella and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Use 5 L of modified f / 2 medium in a self-made photobioreactor, and insert the cell density at 5×10 7 Individual / ml algae liquid 500mL. Into 0.25vvm air, CO 2 The content is 0.03% (v / v), after 15 days of cultivation, the biomass reaches 1.1g / L, CO 2 Absorption rate 6%. The oil content was 21% when cultured to 15 days.

Embodiment 2

[0029] Use 5L of modified f / 2 medium in a self-made photobioreactor, the initial pH is 7.2, the temperature is 27°C±0.5°C, the light intensity is 5000lux, and 0.25vvm air and CO are introduced 2 Mixed gas, where CO 2 The content is 5% (v / v), and the density of inserted cells is about 5×10 7 Individual / mL algae liquid 500mL. After 15 days of cultivation, the biomass reached 2.23g / L, CO 2 The absorption rate is 51%. The oil content was 31% when cultured to 15 days.

Embodiment 3

[0031] Use 5L of modified f / 2 medium in a self-made photobioreactor, the initial pH is 7.2, the temperature is 27°C±0.5°C, the light intensity is 5000lux, and 0.25vvm air and CO are introduced 2 Mixed gas, where CO 2 The content is 10% (v / v), and the density of inserted cells is about 5×10 7 Individual / mL algae liquid 500mL. After 15 days of cultivation, the biomass reached 1.91g / L, CO 2 The absorption rate is 26%. The oil content was 22% when cultured to 15 days.

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Abstract

The invention discloses a strain of chlorella, which is classified to be named as chlorella vulgaris and is preserved in China Centre for Type Culture Collection, and the preservation number is CCTCC NO.: M 209256. The invention also discloses the application of the chlorella in producing microalgae oil and fixing CO2. The chlorella can endure 24.5% of CO2 concentration, the content of grease can reach 17-31%, and the invention has higher CO2 fixing efficiency than ordinary seawater algae, and can obtain high-yield microalgae oil by selecting appropriate culture conditions, so the culture cost of the microalgae can be greatly reduced, and a good production strain is provided for preparing biodiesel by the microalgae oil.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, and in particular relates to a strain of chlorella and its application. Background technique [0002] The exploitation and use of traditional fossil resources has promoted economic and social development, and at the same time produced a large amount of CO 2 Such as greenhouse gases, which further deteriorate the global environment, frequent catastrophic climate, a serious threat to the sustainable development of society. According to World Bank statistics, in the past 30 years, due to CO 2 The greenhouse effect caused by excessive emissions has caused the earth's temperature to rise at a rate of 0.2 degrees Celsius every 10 years. Greenhouse gases have become the most important factor of climate change and pose a serious threat to the global ecological balance. [0003] In the past 20 years, some developed countries have used various physical, chemical and biological methods to study CO ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P7/64B01D53/14C12R1/89
CPCY02E50/13Y02E50/10Y02P20/59
Inventor 黄和张齐高振郑洪立纪晓俊唐小红尹继龙
Owner NANJING UNIV OF TECH
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