Method for producing hydrogen by utilizing facultative anaerobe fermentation

A facultative anaerobic bacteria and fermentation substrate technology, applied in the field of biological hydrogen production, can solve the problems of strict fermentation conditions and complex operation of biological hydrogen production

Inactive Publication Date: 2010-09-08
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the problems of complex operation and strict requirements on fermentation conditions in the biological hydrogen production method in the prior art, the present invention mainly includes a method for hydrogen production by using facultative anaerobic bacteria. The method is under non-strict anaerobic conditions, Enterobacter aerogenes (Enterobacter aerogenes) CICC10293 is used, glucose is used as the fermentation substrate, and the hydrogen production process can be efficiently completed in 20 hours by batch fermentation

Method used

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  • Method for producing hydrogen by utilizing facultative anaerobe fermentation
  • Method for producing hydrogen by utilizing facultative anaerobe fermentation
  • Method for producing hydrogen by utilizing facultative anaerobe fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: Selection and determination of bacterial strain inoculation methods

[0027] The medium components are: peptone 5g / L, beef extract 3g / L, NaCl 5g / L, adjust the initial pH=7.0, and use this as the basic medium; all sterilization is performed at 115°C for 25 minutes;

[0028](1) Scheme 1: prepare the basic medium and sterilize it, inoculate the Enterobacter aerogenes high-concentration bacterial solution in the basic medium with a volume ratio of 2% to 5% (aseptic operation), and inoculate the base medium at a temperature of 30°C and 170r / min After 24-48 hours of shaking culture on a shaking table, transfer the cultured bacterial liquid to the sterilized glucose fermentation substrate with a concentration of 20g / L. The total amount of the fermentation liquid is 450mL. 170r / min shaker shake culture, and connect the hydrogen collection device.

[0029] (2) Scheme 2: Add glucose to the base medium at a ratio of 20g / L as a fermentation substrate, obtain a cultur...

Embodiment 2

[0032] Embodiment 2: Determination of optimal fermentation base component configuration

[0033] According to the result of embodiment 1, on the basis of application scheme two, further optimize fermentation condition, explore the best fermentation medium component configuration, carry out following test:

[0034] Three kinds of fermentation media were prepared respectively, numbered 1 to 3, and their compositions were shown in the table below:

[0035] Medium No.

Glucose 20g / L

Peptone 5g / L

Beef Extract 3g / L

NaCl 5g / L

K 2 HPO 4 1.5g / L

FeSO 4 4H 2 O 0.2g / L

1

+

+

+

+

2

+

+

+

+

+

3

+

+

+

+

+

+

[0036] Adjust the initial pH of the fermentation medium to 7.0, sterilize at 115°C for 25 minutes, inoculate Enterobacter aerogenes high-concentration bacterial liquid at a volume ratio of 2% to 5% (aseptic op...

Embodiment 3

[0038] Embodiment 3: the determination of optimum fermentation substrate concentration

[0039] According to the results of Examples 1 and 2, the possibility of improving the hydrogen production efficiency was explored by changing the initial concentration of the fermentation substrate.

[0040] Prepare basal medium, consisting of: peptone 5g / L, beef extract 3g / L, NaCl 5g / L, K 2 HPO 4 1.5g / L, FeSO 4 4H 2 O 0.2g / L, set 4 groups of parallel experiments, set the initial substrate glucose concentration as 5, 10, 20, 30, 40 and 60g / L respectively, the total fermentation liquid volume is 450mL, other fermentation conditions are all the same. Before the start of the culture, they were purged with nitrogen for 3-5 minutes to an anaerobic or near-anaerobic environment, placed on a shaker at a temperature of 40 ° C, 170 r / min for shaking culture and connected to a hydrogen collection device, and the duration was 20 hours. The gas is collected during the fermentation process, and the...

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Abstract

The invention provides a method for producing hydrogen by utilizing facultative anaerobe fermentation, and in particular relates to two aspects of strain screening and fermentation condition optimization. The screened facultative anaerobe is Enterobacter aerogenes CICC10293; through tests, the optimal fermentation process conditions of a strain are as follows: a culture medium is concretely prepared from 4 to 6g / L of peptone, 2 to 4g / L of beef extract, 4 to 6g / L of NaCl, 1 to 1.5g / L of K2HPO4 and 0.1 to 0.2g / L of FeSO4.4H2O; a fermentation substrate is dextrose, and the concentration is 20g / l; fermentation adopts a mode of batch fermentation; constant temperature shaking culture is carried out under conditions that the pH is 6.0 to 7.0 and the temperature is 40 DEG C, and the shaking frequency is 170r / min; and the culture time is about 20 hours until hydrogen production stops. The hydrogen yield can reach 134mLH2 / 100mL of liquid culture medium, the hydrogen production efficiency is high, the requirement for fermentation environment conditions is low, and the invention has broad application aspect.

Description

technical field [0001] The invention relates to a method for biological hydrogen production, in particular to a method for producing hydrogen by using facultative anaerobic bacteria. Background technique [0002] With the rapid development of the economy and the continuous improvement of people's living standards, the total energy consumption in my country has also increased rapidly. 95% of the commercial energy currently used is fossil energy, but the environmental problems caused by the development and utilization of fossil energy severely restrict sustainable development. In the context of the rapid consumption of fossil energy resources in the world, the increasing environmental pollution and the increasing threat of climate warming, in order to support the Chinese government’s proposal to reduce carbon dioxide emissions per unit of GDP by 40% to 45% compared with 2005 by 2020 In particular, the development and utilization of hydrogen and other renewable energy sources ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12R1/01
Inventor 钱春香袁晓明王瑞兴
Owner SOUTHEAST UNIV
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