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Application of ranunculus japonicus extract in preparing anti-tumor and anti-tumor angiogenesis drugs

A technique for angiogenesis and extraction, which is used in anti-tumor drugs, applications, drug combinations, etc.

Active Publication Date: 2010-09-15
JIANGSU SUDA INVESTMENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After literature research, there is no relevant literature report on the inhibitory effect of buttercup extract on angiogenesis by Chinese scholars

Method used

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  • Application of ranunculus japonicus extract in preparing anti-tumor and anti-tumor angiogenesis drugs
  • Application of ranunculus japonicus extract in preparing anti-tumor and anti-tumor angiogenesis drugs
  • Application of ranunculus japonicus extract in preparing anti-tumor and anti-tumor angiogenesis drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: Inhibitory effect of buttercup extract on tumor angiogenesis in vitro

[0044]Mouse liposarcoma cells SW872 were placed in DMEM medium containing 10% fetal bovine serum at 37°C, 5% CO 2 Incubator cultivation. Take the cells in the logarithmic growth phase, and use 4*10 4 / mL cells were planted in a 24-well plate pre-coated with a layer of fibrin, placed at 37 ° C, 5% CO 2 Incubator for 24 hours. After 24 hours, different concentrations (1-1000ug / mL) of ranunculus extract were added, with the blank culture solution as the control and 5uM 5-Fu as the positive control, after 36 hours of cultivation, the supernatant was carefully removed, and stained with Wright-Gimsa solution. After staining, select a typical area of ​​each well to take pictures. The above experiments were repeated 3 times.

[0045] The result is as Figures 1 to 17 As shown, various buttercup extracts have very good inhibitory effects on angiogenesis of tumor cells in vitro.

Embodiment 2

[0046] Example 2: Inhibitory effect of buttercup extract on the proliferation of various tumor cells

[0047] Place tumor cell lines 2C4G2, U937, 2C4, Dami, Namalva, K562, PMKO102-3#, BAEC, HMY2-CIR in DMEM or RPMI 1640 culture medium containing 10% fetal bovine serum at 37°C, 5% CO 2 Incubator cultivation. The cells in the logarithmic growth phase were taken and seeded in a 96-well plate at an appropriate concentration, wherein the drug was administered while the suspended cells were seeded on the plate, and the drug was administered to the adherent cells 24 hours later. Put at 37℃, 5% CO 2 Incubator for 24 hours. After 24 hours, different concentrations (1-400ug / mL) of ranunculus extract were added to the adherent cells. The blank culture solution with cells was used as the control, and the blank culture solution without cells was used as the blank control. After 46 hours of culture, Alamar blue reagent was added. 10uL / well, placed in a 37°C, 5% CO2 incubator for 2 hours ...

Embodiment 3

[0054] Example 3: Inhibitory effect of buttercup extract on Matrigel tumor angiogenesis in vitro

[0055] Take 150uL of melted BD Matrigel, add it to a 48-well plate, tap it to make it evenly distributed, and incubate at 37°C for 1 hour to make Matrigel polymerize into a gel. Take the tumor cells SW872 and 3B11 in the logarithmic growth phase, and use 4*10 5 / mL cells were mixed with equal volumes of RPMI 1640 culture medium containing different concentrations of ranunculus extract, seeded on Matrigel at 200uL / well, and incubated at 37°C, 5% CO 2 After culturing in the incubator for several hours, stain with Wright-Gimsa staining solution, observe and take pictures under a microscope.

[0056] Such as Figure 18 and Figure 19 As shown, the results showed that the G component of buttercup extract had a good inhibitory effect on the in vitro Matrigel model of two kinds of tumor cells, and it was dose-dependent. In the control group, the blood vessels were thick, and the tub...

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Abstract

The invention discloses the application of ranunculus japonicus extract in preparing drugs, health food or cosmetics for inhibiting angiogenesis. The invention discovers that the ranunculus japonicus extract has activity for inhibiting tumor and tumor angiogenesis for the first time, and the drugs for inhibiting tumor and tumor angiogenesis, which are prepared by utilizing the discovery, can be used for treating or preventing diseases related to tumors or tumor angiogenesis or for tumor chemotherapy and / or adjuvant chemotherapy. The invention skillfully takes the angiogenesis as a target point for treating diseases, studies and clarifies the medical treatment effects and mechanism of the ranunculus japonicus extract, provides scientific foundation and important information for the development of new application and for discovering new target points of the drug effects, and is a novel development direction of traditional Chinese medical theories.

Description

technical field [0001] The invention relates to the application of buttercup extract in the preparation of drugs for inhibiting angiogenesis, health food or cosmetics. Background technique [0002] Blood vessels are necessary for the morphogenesis and function maintenance of normal tissues and organs, and are closely related to human developmental disorders and various diseases caused by various etiologies. Malignant tumors are currently the culprit of people's life and health in our country, and they are also the key scientific and technological projects of the country's "population and health". Tumor neovascularization plays a pivotal role in tumor occurrence, development, metastasis and recurrence. A series of studies have shown that as long as the tumor neovascularization is effectively inhibited or the existing tumor blood vessels are destroyed, tumor formation, metastasis and recurrence can be inhibited. In addition, diseases such as diabetes and rheumatoid arthritis...

Claims

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Application Information

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IPC IPC(8): A61K36/71A61K8/97A23L1/29A61P9/00A61P35/00A61P35/02A61P27/06A61P3/10A61P9/10A61P27/02A61P17/06A61P17/00A61P19/02A61P29/00A61P9/14A61P17/02A61P17/10A61Q19/00A23L33/00
Inventor 周泉生顾振纶曹志飞朱峰妍蒋小岗郭次仪
Owner JIANGSU SUDA INVESTMENT CO LTD
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