Production process for improving production intensity of propionic acid

A technology of production intensity and production process, which is applied in the field of microbial fermentation to produce propionic acid, can solve problems such as regardless of product production intensity, and achieve the effects of increasing production intensity, increasing output, and shortening production time

Active Publication Date: 2010-09-15
JIANGNAN UNIV
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  • Abstract
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Problems solved by technology

Production cannot simply pursue high output (unit is g / L), regardless of the production intensity of the product

Method used

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  • Production process for improving production intensity of propionic acid

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Embodiment

[0022] 1. Seed culture method: Inoculate the preserved glycerol tube strain: P.acidipropionici CGMCC1.2230 in an anaerobic bottle according to the inoculation amount of 1% (250 mL anaerobic bottle with 200 mL seed medium) After the anaerobic bottle was evacuated with a vacuum pump, it was placed in a 30°C incubator and incubated for 48 hours.

[0023] 2. Fermentation tank culture conditions: the seeds cultivated to the logarithmic phase are inoculated in a 7L fermenter (4L liquid capacity) for fermentation according to the 10% inoculum size, and 10% (w / v) Ca(OH) is added by feeding. The pH was maintained at 7.0, the stirring speed was 200rpm, and the temperature control strategy was adopted for fermentation. The temperature was controlled at 37°C for the first 72 hours, and then changed to 30°C for 72 hours. Samples were taken every 6 hours to measure the content of propionic acid, and the production intensity was calculated. Intermittent before and after each sampling Nitroge...

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Abstract

The invention discloses a production process for improving production intensity of propionic acid, which comprises the following steps of: controlling a fermentation temperature to be 37 DEG C at the early stage (0 to 72 hours) of fermentation; and controlling the fermentation temperature to be 30 DEG C from the 72th hour to the fermentation end. Under the control of the strategy, the production intensity of the propionic acid is improved by 45.5 percent (0.278 vs 0.191g/(L.h)), the fermentation time is shortened by 20 percent (150 vs 120h), and the yield of the propionic acid can be improved by 16.8 percent (28.6 vs 33.4g/L). The propionic acid produced by using the strategy can be applied in the fields of food, chemical industry and the like, and the strategy has vital significance for production of other fermented products.

Description

technical field [0001] The invention relates to a process for producing propionic acid by microbial fermentation, in particular to a production process for increasing the production intensity of propionic acid by controlling the temperature of a culture system. Background technique [0002] Propionic acid and its derivatives are widely used, especially in organic synthesis, food, feed, spices, coatings, dyes, medicine and so on. It can be used as a preservative, spice intermediate, pharmaceutical intermediate and organic synthesis intermediate. In addition, it can also synthesize propionate and cellulose propionate. [0003] Propionic acid has great application value, and its production methods mainly include microbial fermentation and chemical synthesis. Since the total cost of the fermentation method is higher than that of the chemical synthesis method, the former cannot completely replace the latter to produce propionic acid. However, with the increasing awareness of gl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/52C12R1/01
Inventor 陈坚堵国成李平顺刘龙朱云峰李江华姜藜藜
Owner JIANGNAN UNIV
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