Antibody indirect immunofluorescence test method for distinguishing immune animal infected with influenza A virus

Abstract

The invention relates to an antibody indirect immunofluorescence test method for distinguishing whether an immune animal is infected with influenza A virus or not. The method comprises the following steps of: expressing NA protein of influenza A virus as antigen with insect baculovirus; establishing the antibody indirect immunofluorescence test method; and detecting the serum antibody of the NA protein of the influenza A virus. The expressed protein can be directly used as the antigen without purification. The method established by the invention is a detection method matched with the marking vaccine for the influenza A virus, which is used for distinguishing whether the animal vaccinated with the influenza A virus marking vaccine, such as birds, pigs and the like, are infected with corresponding subtype influenza virus or not; as a screening method, the method can be used for detecting the clinical serum sample and determining whether the animal vaccinated with the influenza A virus marking vaccine, such as the birds, pigs and the like, are infected with the corresponding subtype influenza virus or not; and therefore, corresponding preventative measures can be taken as soon as possible so as to eliminate and kill the corresponding epidemic clinical influenza A virus.

Description

technical field [0001] The invention relates to an antibody indirect immunofluorescence test method for identifying whether animals vaccinated with type A influenza "marker" vaccines, such as poultry and pigs, are infected with corresponding subtype influenza viruses, in particular to a method for detecting serum antibodies to type A influenza virus NA protein The indirect immunofluorescence test method is a supporting detection method for type A influenza "marked" vaccines. Background technique [0002] Type A influenza, such as H5N1 subtype avian influenza and H1N1 influenza A, is a serious zoonotic disease that can cause huge economic losses to the poultry industry and cause human death. It is prevalent in many countries in the world, among which Including my country. Classical measures to prevent influenza A (such as avian influenza H5N1 subtype) include strict biosecurity measures, restricting the movement of animals at risk of influenza virus infection, and culling vi...

AI Technical Summary

Problems solved by technology

[0005] 1. High cost

"Sentinel" animals must be specific pathogen-free animals, that is, they must not contain certain pathogens, such as influenza A virus, to be used as "sentinel" animals. Before using "sentinel" animals, they must be kept in a special environment. Therefore, " Sentinel animals are expensive and require a certain amount of

[0006] 2. Difficult to use on a large scale

In order to identify whether animal groups are infected with wild virus, a certain proportion of "sentinel" animals must be released to each group of animals. This is not feasible in actual operation and can only be implemented for the highest risk animal groups. Therefore, it is difficult to apply on a large scale

[0007] 3. Can only be identified at the group level

The use of "sentinel" animals can only identify whether the animal group is infected with wild virus at the group level, and cannot understand and grasp the proportion and status of the animal group infection

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