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Preparation method of selenium protein in selenium-rich ganoderma

A selenoprotein and selenium-enriched technology, which is applied to the preparation methods of peptides, chemical instruments and methods, organic chemistry, etc., can solve the problems of low purity of selenoproteins and achieve the effect of good purity

Inactive Publication Date: 2010-10-06
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to solve the problem of low purity of selenoproteins obtained by existing methods, and to provide a method for preparing selenoproteins in selenium-enriched Ganoderma lucidum

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  • Preparation method of selenium protein in selenium-rich ganoderma
  • Preparation method of selenium protein in selenium-rich ganoderma

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specific Embodiment approach 1

[0008] Specific embodiment 1: The preparation method of selenoprotein in selenium-enriched Ganoderma lucidum according to the present embodiment is carried out according to the following steps: 1. Soak 10-15 g of selenium-enriched Ganoderma lucidum fruiting bodies in deionized water, and stir at 0-10°C for 7~ 10 hours, then suction filtration to obtain filtrate A, cleaning the residue after suction filtration with deionized water to obtain filtrate B, mixing filtrate A and filtrate B to obtain a mixed solution, then adding acetic acid to the mixed solution until the pH is 4.0 ~ 4.5, and then Add ammonium sulfate accounting for 20%~30% of the mass percentage of the mixed solution, let stand at 3~5°C for 10~14 hours, then centrifuge at 4500~5500r / min for 15~25min to remove the precipitate, and then pour Add ammonium sulfate which accounts for 70%~90% of the mass percentage of the centrifuged supernatant to the centrifuged supernatant, let it stand at 3~5°C for 10~14 hours, and th...

specific Embodiment approach 2

[0019] Embodiment 2: This embodiment differs from Embodiment 1 in that in Step 1, 12-14 g of selenium-enriched Ganoderma lucidum fruiting bodies are soaked in deionized water, and stirred at 2-8°C for 8-9 hours. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0020] Embodiment 3: The difference between this embodiment and Embodiment 1 is that in step 1, 13 g of selenium-enriched ganoderma lucidum fruiting bodies were soaked in deionized water, and stirred at 5° C. for 8.5 hours. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

The invention provides a preparation method of selenium protein in selenium-rich ganoderma, which relates to a preparation method of protein. The invention solves the problem that the selenium protein obtained by the traditional method has low purity. The method comprises the following steps of: (1) carrying out ultrafiltration, and depositing ammonium sulfate to prepare selenium-rich ganoderma sporocarp water-soluble crude protein; (2) mixing the selenium-rich ganoderma sporocarp water-soluble crude protein and a Tris-HCl buffer solution, and filtering; (3) carrying out separation and purification by a sephadex column; (4) carrying out separation and purification by an anion exchange chromatography column; and (5) carrying out separation and purification by a hydrophobic chromatography column to obtain the selenium protein in the selenium-rich ganoderma. The selenium content in the selenium protein prepared by the invention is about 6mg / g; compared with the selenium protein prepared by the traditional method, the selenium protein prepared by the method of the invention has high selenium content; and meanwhile, a gel filtration method is used for inspecting the purity of the selenium protein prepared by the implementation mode, and an elution curve shows single protein peaks, thus the selenium protein prepared by the invention has good purity.

Description

technical field [0001] The invention relates to a protein preparation method. Background technique [0002] Selenium has many biological functions. The low selenium state of the human body and the nutritional status of selenium are related to arthritis, cancer, cardiovascular disease, cataract, cholestasis, Crohn's disease, sudden infant death syndrome, cystic fibrosis, diabetes, The incidence of more than 40 diseases such as goiter, immunodeficiency, Kaschin-Beck disease, Keshan disease, lymphocytic anemia, muscular dystrophy, stroke and ulcerative colitis are closely related. Ganoderma lucidum has been hailed as a good product for prolonging life since ancient times, and has multiple biological functions (Huang Weiguang et al., 1993). However, the content of organic selenium in normal Ganoderma lucidum is relatively low. Use the selenium-enriching ability of Ganoderma lucidum to convert inorganic selenium into organic selenium, and enrich it with a relatively high content...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/36C07K1/30C07K1/20C07K1/18C07K1/16
Inventor 杜明胡小松赵广华
Owner HARBIN INST OF TECH
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