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Method for producing lactic acid by using sugar liquor fermentation

A technology of sugar clear liquid and lactic acid, which is applied in the field of lactic acid and its fermentation process, can solve the problems of long fermentation period, low acid production and acid production rate, and inability to produce lactic acid on a large scale, and achieve the effect of high fermentation efficiency and short cycle

Active Publication Date: 2012-07-04
河南金丹乳酸科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] (1) The fermentation period is long, and the fermentation period of most enterprises is about 70 hours;
[0005] (2) Acid production and acid production rate are low. Domestic large-scale production of acid production is less than 120g / L. World-scale lactic acid production can reach 130-150g / L, and acid production rate is generally 1.5-2g / L·h ;
[0007] Therefore, most lactic acid enterprises in my country cannot produce lactic acid on a large scale

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] In this embodiment, the method for producing lactic acid by fermenting sugar serum comprises the following steps: (1) Raw material preparation: according to the volume ratio, sugar serum: corn steep liquor solution: yeast extract = 100: 0.015: 0.004 is added to the fermenter, so The total sugar content of the sugar clear liquid is 210g / L, the DE value is 92%, and the DX value is 90%. In the described corn steep liquor solution: the dry matter is 40%, the protein is 45%, and the acidity is 14%; (2 ) heat sterilization: the temperature in the fermenter is 115°C, the pressure is 0.07Mpa, and the heat sterilization time is 15 minutes; (3) adjust the pH of the feed liquid: use Ca(OH) 2 The saturated solution adjusts the pH of the feed liquid to 5.4, if the Ca(OH) 2 Add too much saturated solution, then use 80% lactic acid or 35% hydrochloric acid to adjust the pH of the feed liquid; (4) add lactic acid bacteria to ferment: the temperature of the feed liquid obtained in step ...

Embodiment 2

[0020] In this embodiment, the method for producing lactic acid by fermenting sugar serum comprises the following steps: (1) raw material preparation: according to the volume ratio, sugar serum: corn steep liquor solution: yeast extract = 100: 0.025: 0.013 is added to the fermenter, so The total sugar content of the sugar clear liquid is 240g / L, the DE value is 100%, and the DX value is 100%. In the described corn steep liquor solution: the dry matter is 50%, the protein is 52%, and the acidity is 10%; (2 ) heat sterilization: the temperature in the fermenter is 121°C, the pressure is 0.1Mpa, and the heat sterilization time is 45 minutes; (3) adjust the pH of the feed liquid: use Ca(OH) 2 The saturated solution adjusts the pH of the feed liquid to 5.6, if the Ca(OH) 2 Add too much saturated solution, then use 80% lactic acid or 35% hydrochloric acid to adjust the pH of the feed liquid; (4) add lactic acid bacteria to ferment: the temperature of the feed liquid obtained in step...

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Abstract

The invention relates to a method for producing lactic acid by using sugar liquor fermentation, which comprises the following steps of: (1) preparing raw materials, namely adding sugar liquor, corn pulp solution and yeast extract into a fermentation tank in a volume ratio of 100: 0.015-0.025: 0.004-0.013; (2) heating and sterilizing the raw materials; (3) adjusting the pH of the solution to be between 5.4 and 5.6; and (4) adding lactic acid bacteria into the solution to perform fermentation, namely cooling the solution obtained in the step (3) to between 48 and 50 DEG C, adding the lactic acid bacteria in a volume which is 10 to 15 percent of the total volume of the solution in the fermentation tank, keeping the temperature in the fermentation tank between 48 and 50 DEG C, continuously introducing sterile air into the fermentation tank in a flow of 0.75 to 4m<3> / h in every ton of solution, keeping the pressure in the fermentation tank between 0.4 and 0.8MPa, stirring the solution, adding saturated solution of Ca(OH)2 into the solution to keep the pH of the solution between 6.0 and 6.2, and detecting the glucose content of the solution, wherein when the glucose content is 0, the fermentation is finished. The method of the invention reduces the fermentation period, and improves the fermentation efficiency; and after the fermentation is finished, the solution contains low residual total sugar and residual reducing sugar and high produced acid.

Description

technical field [0001] The invention relates to lactic acid and its fermentation process, in particular to a method for producing lactic acid by fermenting high-concentration sugar clear liquid. Background technique [0002] Lactic acid is an organic acid obtained from agricultural and sideline products through microbial fermentation and extraction. It is the monomer raw material of polylactic acid, a degradable and environmentally friendly material. It is widely used in food, beverage, medicine, health products, chemical industry, degradable plastics and many other fields. [0003] The production of lactic acid mainly includes "chemical synthesis", "enzymatic synthesis" and "fermentation production", among which the most widely used is the production of lactic acid by fermentation. At present, in the production of lactic acid by fermentation, many enterprises have low fermentation efficiency due to immature fermentation process indicators and unreasonable control of raw ma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/56C12R1/225
Inventor 张云飞崔耀军张兴龙胡中华马凯歌郭廷朱守林王新姜建友杨峰梁丽
Owner 河南金丹乳酸科技股份有限公司