SDS-PAGE (Sodium dodecyl sulfate-polyacrylamide gel electrophoresis) rapid decoloring method and special decoloring cup thereof

Abstract

The invention relates to an SDS-PAGE rapid decoloring method and a special decoloring cup thereof. The method comprises the following steps: (1) gel cleaning: after electrophoresis gel dyeing is ended, cleaning the gel for 2-3 times with deionized water and the like; (2) pre-decoloring: putting the gel in the decoloring cup holding water, heating to the temperature about 80 DEG C and then discharging the water in the decoloring cup, and moreover, repeating the process for 2 times; and (3) decoloring: adding enough water to the decoloring cup; heating to the temperature which is 80 DEG C and then changing the water; then heating; recycling in such a way until the background is clean and strips are clear to finish the decoloring. The invention has the advantages that: 1. the deionized water and the like is used as a decoloring solution instead of a methanol-glacial acetic acid solution, and therefore, the cost is low and environmental pollutions can not be generated; 2. the whole decoloring process is finished by matching with a special decoloring device and utilizing a heating method in 30-40 min, i.e. the method is convenient and rapid and accelerates test progress; 3. the method has simple operation and can very conveniently conduct a decoloring test without preparing a special decoloring solution by only utilizing the existing heating equipment of a laboratory and adopting the special decoloring device.

Description

technical field [0001] The invention belongs to electrophoresis gel decolorization technology, and specifically relates to a SDS-PAGE rapid gel decolorization method and a special decolorization cup. The invention uses distilled water or deionized water as a decolorization agent, combined with a special decolorization instrument, and uses it at a specific temperature. Decolorize the gel by heating the decolorizer. Background technique [0002] SDS-polyacrylamide gel electrophoresis is the introduction of SDS (sodium dodecyl sulfonate) into the polyacrylamide gel system. SDS combines with the hydrophobic part of the protein, breaks intramolecular and intermolecular hydrogen bonds, and destroys the protein. The folded structure of the secondary and tertiary structure makes it stable in a broadly homogeneous solution. In a certain concentration of SDS solution containing a strong reducing agent, the protein is proportionally combined with the SDS molecule to form a negatively ...

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Problems solved by technology

However, we found in the experiment that heating in tap water can easily turn the gel into white, which cannot be recovered, and the results cannot be verified. This method is not very feasible.

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