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Rapid detection method based on gold magnetic particle-labeled immunochromatography

A technology of gold magnetic particles and detection methods, which can be used in measurement devices, analytical materials, biological tests, etc., and can solve the problems of complicated operations, subjective differences, and narrow application fields.

Active Publication Date: 2014-12-17
XIAN GOLDMAG NANOBIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention proposes a rapid detection method using gold magnetic particles to label immunochromatographic detection strips, aiming to solve the problem of subjective differentiation, low detection accuracy and sensitivity, complicated operation and poor application of traditional immunochromatographic detection methods in clinical practice. Narrow issues

Method used

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  • Rapid detection method based on gold magnetic particle-labeled immunochromatography
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  • Rapid detection method based on gold magnetic particle-labeled immunochromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Application of gold magnetic particle-labeled rapid immunochromatographic test strips for early pregnancy detection based on the sandwich method (in the field of health testing)

[0051] 1. The β-antibody of human chorionic gonadotropin HCG is labeled with gold magnetic particles, and dissolved in the chromatography working solution:

[0052] 1.1) Take the gold magnetic particles, equilibrate for 3 times with pH=7, 0.01M phosphate buffer, add the β-antibody of human chorionic gonadotropin HCG at the ratio of 200 μg / mg gold magnetic particles, and then pH=7, 0.01M phosphate buffer solution balance treated gold magnetic particles, placed in 35 ℃, 180rpm constant temperature oscillator fully reacted for 30min;

[0053] 1.2) After the reaction is completed, separate on the magnetic separator for 3 minutes, discard the supernatant, and wash with pH=7, 0.01M phosphate buffer containing 0.5% Tween20 to remove protein molecules that are not bound to the surface of the gold magn...

Embodiment 2

[0065] Detection of cardiac troponin by using gold magnetic particle-labeled rapid immunochromatographic test strips based on the sandwich method (in the field of disease detection)

[0066] 1. Gold magnetic particle-labeled cardiac troponin monoclonal antibody, and dissolved in the chromatography working solution:

[0067] 1.1) Take 1 mg of gold magnetic particles, equilibrate 3 times with pH=7, 0.01M phosphate buffer, add 1 mg / ml cardiac troponin monoclonal antibody mAb2-19C7, 200 μg, into the gold magnetic particles, and place at 35°C , 180rpm constant temperature oscillator fully reacted for 30min;

[0068] 1.2) After the reaction is completed, separate on a magnetic separator for 3 minutes, suck off the supernatant, wash with pH=7, 0.01M phosphate buffer containing 0.5% Tween20, and remove unlabeled cardiac troponin antibody I molecules; repeat the above steps three times.

[0069] 1.3) Blocking: Add 1ml of blocking agent to the gold magnetic particles immobilized with ...

Embodiment 3

[0080] Detection of aflatoxin in food using gold magnetic particles based on competition method (food safety detection field)

[0081] 1. Anti-aflatoxin monoclonal antibody clone 3A7 is labeled with gold magnetic particles and dissolved in the chromatography working solution:

[0082] 1.1) Take 1 mg of gold magnetic particles, equilibrate 3 times with pH=7, 0.01M phosphate buffer, add 1 mg / ml anti-aflatoxin monoclonal antibody clone 3A7, 200 μg, into the gold magnetic particles, place at 35 Fully react in a constant temperature oscillator at 180 rpm for 30 minutes;

[0083] 1.2) After the reaction is completed, separate on a magnetic separator for 3 minutes, suck off the supernatant, wash with pH=7, 0.01M phosphate buffer containing 0.5% Tween20, and remove the unlabeled anti-aflatoxin monoclonal antibody clone 3A7 ;Repeat the above steps three times;

[0084] 1.3) Blocking: Add 1ml of blocking agent to the gold magnetic particles immobilized with cardiac troponin antibody I...

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Abstract

The invention provides a rapid detection method based on gold magnetic particle-labeled immunochromatography, aiming at solving the problems that a detection method of a traditional immunity chromatography analysis method is easy to generate subjective differentiation and has low detection accuracy and sensitivity, complicated operation and narrow application field in clinical practice. The basic idea of the detection method is as follows: the gold magnetic particles coupled with an antibody, a ligand and an antigen can rapidly and quantitatively and high sensitively detect an assay in a matched working solution and an immunochromatography device in future. The detection method can interpret visually according to the characteristic colors of the gold magnetic particles and can detect the assay quantitatively according to the strength of the signals of the gold magnetic particles, is simple, has low cost, and can realize disease detection on human and animals and plants, environmental detection, food safety detection, drug and medicine abuse detection and the like.

Description

technical field [0001] The invention relates to an immunochromatographic analysis method, in particular to a rapid detection method using gold magnetic particle-labeled immunochromatography, which is mainly used in the detection fields of hormones, proteins, viruses, drugs, bacteria, drug molecules, toxins, and heavy metals. Background technique [0002] The existing colloidal gold and latex particle-labeled immunochromatographic analysis methods have been widely used in clinical medical testing. After adding the antigen or antibody to the sample to be tested, the diafiltration or capillary action of the microporous membrane is the combination of the coated antigen or antibody on the target film in the sample, and the colloidal gold itself reacts with it to form the colloidal gold itself. The color (red) or latex particles (blue) can be detected by visual inspection. However, for some samples with extremely low antigen or antibody content, the color of the marker is so ligh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/531G01N33/577G01N33/76G01N33/72G01N33/558
Inventor 崔亚丽石峰惠文利
Owner XIAN GOLDMAG NANOBIOTECH
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