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Method for somatic cell embryogeny and plant regeneration of medicinal plant schisandga chinensis baill

A technology of Schisandra chinensis and somatic embryos is applied in the field of somatic embryogenesis and plant regeneration of Schisandra chinensis, which can solve the problems of long induction period and low induction rate of somatic embryos, overcome the low germination rate of seeds and improve the embryo shape. body induction rate

Inactive Publication Date: 2010-12-08
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the invention is to provide a method to induce the embryoid body of Schisandra chinensis through the mature embryo of seeds in my country and the low induction rate of somatic embryos and long induction period to provide a kind of in vitro condition with plant growth regulator to induce the embryoid body. Formation of somatic embryos of Schisandra chinensis and method for plant regeneration

Method used

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  • Method for somatic cell embryogeny and plant regeneration of medicinal plant schisandga chinensis baill
  • Method for somatic cell embryogeny and plant regeneration of medicinal plant schisandga chinensis baill
  • Method for somatic cell embryogeny and plant regeneration of medicinal plant schisandga chinensis baill

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Experimental program
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Effect test

Embodiment 1

[0026] A. Rinse the mature Schisandra chinensis seeds with running water for four hours, put them into 70% alcohol for surface disinfection for 30 seconds, put them into 0.1% mercuric chloride aqueous solution for disinfection for 8 minutes, and then wash them with sterile water for 4 to 6 times. Then the mature embryos were peeled off and inoculated into 2,4-D (chlorinated phenoxyacetic acid) or 0.1-2.0 mg / L TDZ (thidiazuron) with an additional growth regulator of 0.5-11 mg / L, and the mass-to-volume ratio was On the MS medium of 3% sucrose and 0.7% agar, the culture temperature is 23±1°C, the relative humidity is 70%, and the culture is dark for 6-10 weeks to induce the callus.

[0027] B. Transfer the callus to any one of the additional growth regulators 0-2.0mg / L 2,4-D, 0.1-2.0mg / L TDZ, 0.1-0.3mg / L ZT or any combination of any two / 2MS medium, in which the additional mass volume ratio of 3% sucrose and 0.7% agar was cultured at a culture temperature of 23±1°C and a photoper...

Embodiment 2

[0031] The difference between this embodiment and Example 1 is that in step A, 0.5-11 mg / L2,4-D of growth regulator is added to the culture medium. Among them, the optimal concentration of additional 2,4-D is 2.0mg / L. Other steps are identical with embodiment 1.

Embodiment 3

[0033]The difference between this embodiment and Example 1 is: 0.1-2.0 mg / L TDZ is added to the medium in step A. Among them, the optimal concentration of additional TDZ is 1.0mg / L. Other steps are identical with embodiment 1.

[0034] According to the test, 2.0mg / L 2,4-D and 1.0mg / L TDZ were added to each liter of medium. The test results of the influence of 2,4-D, TDZ concentration on the callus induction rate are shown in Table 1:

[0035] Table 1 Effect of 2,4-D, TDZ on callus induction rate

[0036]

[0037] It can be seen from this table that the callus induction rate is up to 94.7%.

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Abstract

The invention discloses a method for somatic cell embryogeny and plant regeneration of medicinal plant schisandga chinensis baill, which is characterized by comprising the following steps of: A, performing sterilization pretreatment on seeds of the schisandga chinensis baill, peeling whole mature embryos and inoculating the embryos onto an MS culture medium added with an additive growth regulator consisting of 3 mass volume percent of saccharose and 0.7 mass volume percent of agar, culturing the embryos in dark at a culture temperature of 23+ / -1 DEG C and a relative humidity of 70 percent for 6 to 10 weeks and inducing calluses; B, transferring the calluses to the half MS culture medium added with the additive growth regulator consisting of 3 mass volume percent of saccharose and 0.7 mass volume percent of agar, culturing the calluses at a culture temperature of 23+ / -1 DEG C and under a condition of a photoperiod of 16 hours of lighting and 8 hours of dark for 25 to 40 days, and inducing embryonic calluses and embryoids; C, separating the embryos in a cotyledon period, subculturing the somatic cell embryos on the culture medium which is identical with that of the step B for four weeks to obtain regeneration plants; and D, hardening the regeneration plants and then transplanting the regeneration plants to obtain schisandga chinensis baill seedlings. The method has the advantages of inducing the formation of the somatic cell embryos by using the plant growth regulator in vitro, overcoming the defects of low sprouting rate and sand storage, improving the induction rate of the embryos, providing a new technological approach for the excellent cloning of the schisandga chinensis baill and laying a basis for the gene storage and the genetic modification of the schisandga chinensis baill.

Description

technical field [0001] The invention relates to a method for somatic embryogenesis and plant regeneration of medicinal plants, in particular to a method for somatic embryogenesis and plant regeneration of Schisandra chinensis. Background technique [0002] Natural medicinal plant resources are the precious wealth left by nature to human beings and the material basis for human survival. With the growth of population and the increase of human demand for medicinal plants, humans have carried out predatory exploitation of wild plant resources, resulting in the depletion of many plant resources. Therefore, it has become one of the research hotspots in the field of plant biotechnology to deeply study natural medicinal plants and cultivate cells, tissues or organs to meet the huge demand for natural products. Among them, somatic embryogenesis is a mature biotechnology Means are widely used for rapid plant reproduction. [0003] The advantage of rapid propagation of somatic embryo...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 朴钟云李宏博郎文香傅俊范
Owner SHENYANG AGRI UNIV
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