Extraction process for preparing nisin
A technology of nisin and extraction process, which is applied to the preparation method of peptides, decapeptides, organic chemistry, etc., can solve the problems of affecting product quality, high unit cost, and human poisoning, so as to reduce energy costs and reduce energy consumption. The effect of low consumption and cost
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specific Embodiment approach 1
[0008] Specific embodiment one: the extraction process of preparing nisin in this embodiment is realized according to the following steps: one, the fermented liquid containing 8000~15000IU / ml nisin is squeezed into the acidification tank, and the hydrochloric acid with a volume concentration of 36% Adjust the pH value to 2.5, raise the temperature to 80-85°C for acidification and analysis for 10-30 minutes, then cool down to 20-40°C to obtain the acidification liquid; Inject compressed air until the nisin content in the eluate is lower than 5000IU / ml, then pass through the filter device and enter the receiver, and concentrate to obtain a concentrated fermented liquid containing 200000~300000IU / ml nisin; 3. Concentrated fermented liquid Pump into the organic membrane, wash and filter with deionized water to obtain a high-purity concentrated fermentation broth containing 120000~150000IU / ml nisin; 4. Dilute the high-purity concentrated fermentation broth with deionized water until...
specific Embodiment approach 2
[0011] Embodiment 2: The difference between this embodiment and Embodiment 1 is that in step 1, the fermented liquid containing 8000 IU / ml nisin is poured into the acidification tank. Other steps and parameters are the same as those in Embodiment 1.
specific Embodiment approach 3
[0012] Specific embodiment 3: The difference between this embodiment and specific embodiment 1 is that in step 1, the fermented liquid containing 15000 IU / ml nisin is poured into the acidification tank. Other steps and parameters are the same as those in Embodiment 1.
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