Extraction process for preparing nisin

A technology of nisin and extraction process, which is applied to the preparation method of peptides, decapeptides, organic chemistry, etc., can solve the problems of affecting product quality, high unit cost, and human poisoning, so as to reduce energy costs and reduce energy consumption. The effect of low consumption and cost

Inactive Publication Date: 2010-12-08
张国峰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of low yield and high unit cost in the existing nisin extraction process, and the general use of organic solvents for extraction or elution, which affects the product quality and is toxic to the human body, and provides A kind of extraction process for preparing nisin

Method used

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Examples

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specific Embodiment approach 1

[0008] Specific embodiment one: the extraction process of preparing nisin in this embodiment is realized according to the following steps: one, the fermented liquid containing 8000~15000IU / ml nisin is squeezed into the acidification tank, and the hydrochloric acid with a volume concentration of 36% Adjust the pH value to 2.5, raise the temperature to 80-85°C for acidification and analysis for 10-30 minutes, then cool down to 20-40°C to obtain the acidification liquid; Inject compressed air until the nisin content in the eluate is lower than 5000IU / ml, then pass through the filter device and enter the receiver, and concentrate to obtain a concentrated fermented liquid containing 200000~300000IU / ml nisin; 3. Concentrated fermented liquid Pump into the organic membrane, wash and filter with deionized water to obtain a high-purity concentrated fermentation broth containing 120000~150000IU / ml nisin; 4. Dilute the high-purity concentrated fermentation broth with deionized water until...

specific Embodiment approach 2

[0011] Embodiment 2: The difference between this embodiment and Embodiment 1 is that in step 1, the fermented liquid containing 8000 IU / ml nisin is poured into the acidification tank. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0012] Specific embodiment 3: The difference between this embodiment and specific embodiment 1 is that in step 1, the fermented liquid containing 15000 IU / ml nisin is poured into the acidification tank. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

The invention relates to an extraction process for preparing nisin, and solves the problems of low yield, high unit cost, and influence on product quality and harm to human bodies because an organic solvent is generally adopted for extraction or elution in the conventional extraction process of the nisin. A method comprises the following steps of: 1, pouring fermentation liquor into an acidifyingtank for acidifying and analyzing to obtain acidified liquid; 2, pouring the acidified liquid into an aeration tank for aeration, filtering to enter a receiver and concentrating the fermentation liquor; 3, pumping the concentrated fermentation liquor into an organic membrane for filtering to obtain high-purity concentrated fermentation liquor; and 4, diluting the high-purity fermentation liquor, adding edible salt and drying. The aeration is taken as the core of the extraction process; the process is simple and direct; process loss and energy consumption are reduced; the yield is 70 to 80 percent; the energy cost is reduced by 30 percent; and the organic solvent is not used, and substances harmful to the human bodies and environment do not exist.

Description

technical field [0001] The invention relates to an extraction process for preparing coccin. Background technique [0002] Nisin (NISIN) can inhibit and kill Galanz-positive bacteria and their spores. As an organic polypeptide, it can be degraded by the human body. As a biological preservative, it has begun to enter the food processing industry. On January 19, 1990, the Food Supervision Office of the Ministry of Health of my country issued a certificate for the use of Nisin as a food preservative. On March 29, 1990, Nisin was included in the 1990 supplementary variety of the international GB2760-86, and the quality standard was stipulated. [0003] At present, the manufacturers of nisin mainly use resin exchange, membrane filtration and other technologies for extraction, but due to the high price of raw materials used, the complex process, low yield and high unit cost, organic solvent extraction or elution is generally used , which affects the product quality and is toxic to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/315C07K1/14
Inventor 张国峰
Owner 张国峰
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