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Plant nitrogen utilization efficiency related protein TaNF-YA1 and coding gene and application thereof

A technology for encoding genes and proteins, which can be used in plant gene improvement, application, plant peptides, etc., and can solve the problems of difficult functional gene research and large genome in wheat.

Active Publication Date: 2012-08-22
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

The genome of wheat is huge and has not been completely sequenced yet, which brings some difficulties to the study of functional genes of wheat

Method used

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  • Plant nitrogen utilization efficiency related protein TaNF-YA1 and coding gene and application thereof
  • Plant nitrogen utilization efficiency related protein TaNF-YA1 and coding gene and application thereof
  • Plant nitrogen utilization efficiency related protein TaNF-YA1 and coding gene and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1, cultivating transgenic plants and detection thereof

[0049] 1. Breeding transgenic plants

[0050] 1. Acquisition of gene (TaNF-YA1)

[0051] The total RNA of wheat Xiaoyan 54 (Henan Zhongke Seed Industry Co., Ltd.) was extracted, and the whole genome cDNA of wheat was obtained by reverse transcription. As a template, the upstream primer with XbaI restriction site: 5'-CT TCTAGA ATGCTTCTCCCCTCTTCT-3' and downstream primer with BamHI restriction site: 5'-TT GGATCC TTACGTATACCGGTCGCTCGA-3' was used as a primer for PCR amplification. The PCR system was 40 μl in total, including 4 μl of template cDNA, 1 μl of KOD-plus-DNA polymerase (purchased from TOYOBO Company), 10 × PCR buffer for KOD-plus-4 μl, dNTPs ( 2mM each) MgSO 4 (25mM) upstream and downstream primers The reaction system was supplemented with double distilled water to The PCR reaction program was: 98°C for 2min, (98°C for 30sec, 58°C for 30sec, 68°C for 45sec)×35cycles. After the react...

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Abstract

The invention discloses plant nitrogen utilization efficiency related protein TaNF-YA1 and coding gene and application thereof. The plant nitrogen utilization efficiency related protein provided by the invention is named as TaNF-YA1, is from wheat and is protein of the following 1) or 2): 1) protein consisting of an amino acid sequence expressed as a sequence 2 in a sequence table; and 2) protein, which consists of an amino acid residue sequence subjected to substitution and / or deletion and / or addition of one or more amino acid residues in the sequence 2 of the sequence list, is related with the plant nitrogen utilization efficiency and is derived from the 1). The TaNF-YA1 gene influences the nitrogen absorption and utilization, and the over expression of the gene increases the lateral root number and total lateral root length of arabidopsis thaliana.

Description

technical field [0001] The invention relates to protein TaNF-YA1 related to plant nitrogen utilization efficiency, its coding gene and application. Background technique [0002] Wheat is an important food crop in my country. Regardless of the cultivated area or the total output, my country is the largest wheat producer in the world. In the early days of the founding of new China, China's wheat yield was very low, only 0.73 tons / ha (48.8 kg / mu) in 1952, and reached 4.55 tons / ha (303.3 kg / mu) in 2006, an increase of 5.2 times, one of the important The reason is the increase in the use of chemical fertilizers. According to statistics, chemical fertilizers account for 32% of agricultural production, 25% of agricultural production costs, and 50% of all agricultural production material costs. At present, the annual nitrogen fertilizer consumption in my country exceeds 24Mt (pure nitrogen), accounting for more than 30% of the world's annual nitrogen fertilizer consumption (Ju et...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N1/15C12N1/19C12N1/21C12N5/10C12N15/11A01H5/00
Inventor 童依平曲宝原何雪赵学强李振声李滨
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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