Method and instrument of ultrasonic and electric field superposition target medicine penetration
A technology of ultrasound and electric field, which is applied in the field of superimposed ultrasound and electric field target drug penetration, which can solve the problems of tissue damage, dosage and site restrictions of drug administration, and achieve the effect of improving the effect and painless action process
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Embodiment 1
[0020] Parameter scheme:
[0021] Ultrasonic frequency: 20KHz,
[0022] Ultrasonic power: 200mW / cm 2 ,
[0023] Ultrasonic output form: pulsating, period 20ms, duty ratio 1:1,
[0024] Electroporation: square wave, duty ratio 1:1, period 30ms,
[0025] Pore forming voltage: 100V
[0026] Ion introduction waveform: 2000Hz square wave, duty ratio 1:1,
[0027] Modulation waveform: 100Hz sine half wave,
[0028] Peak voltage: 30V
[0029] Output time: 30min
[0030] Output program: 10s electroporation pilot pulse and subsequent ultrasonic conductance superimposed output repeated every 10min.
[0031] The structure process of the instrument is:
[0032] The network power input cable is connected to the power socket fixed on the rear panel of the instrument shell, the fuse is connected between the power socket and the primary input line of the transformer fixed on the bottom plate of the shell, and the bridge rectifier circuit of the main circuit board is connected to th...
Embodiment 2
[0033] Example 2: In vivo test, using the instrument obtained in Example 1, to conduct ultrasound conductance drug penetration and tissue drug concentration determination in rabbits.
[0034] Take a healthy rabbit with a body weight of 2.0±0.5kg, fix the hairless skin on the buttocks and paste a hollow patch, add 5% ketoprofen gel to the hollow area, immerse the treatment head in the gel, and apply a 100V pore-forming pulse 5 One, 20KHz ultrasonic wave and 30V conductance pulse for 10min, after the end, the rabbit was killed quickly, the tissue of the administration site was taken and ground to extract the interstitial fluid containing ketoprofen, the components were recovered, and detected by high performance liquid phase.
[0035] Rabbits in the control group were orally administered with the same dose of ketoprofen, and sacrificed 10 minutes later. Others are the same as the experimental group.
[0036] Liquid phase conditions:
[0037] Mobile phase: KH 2 PO 4 (0.07M, pH4...
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