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Method for producing flavonoid compound by streptomyces

A technology of flavonoids and streptomyces, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of difficult extraction and purification, limited development and utilization of flavonoids, complex structures of flavonoids, etc. achieve the effect of overcoming flavonoids

Inactive Publication Date: 2011-10-05
HUNAN AGRICULTURAL UNIV
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  • Claims
  • Application Information

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Problems solved by technology

Flavonoids are widely found in plants in nature. Polyphenols and almost all plants contain a certain amount of flavonoids in flowers, leaves and stems. However, due to the seasonal influence of plant resources, the complex structure of flavonoids Difficulty in extraction and purification from plants, unstable antibacterial effect of crude extracts of plant flavonoids, etc., limit the further development and utilization of flavonoids
Flavonoids from Streptomyces, previously unreported

Method used

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  • Method for producing flavonoid compound by streptomyces
  • Method for producing flavonoid compound by streptomyces
  • Method for producing flavonoid compound by streptomyces

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Embodiment Construction

[0017] combined reference figure 1 , the present invention will be further described.

[0018] Preparation of Streptomyces viridans fermentation broth:

[0019] Get Streptomyces viridans HNS2-2 and cultivate it in Gaoshi No. 1 slant medium, then inoculate it in a 300mL Erlenmeyer flask with 30mL seed medium (20g of soluble starch, 2g of yeast extract and 1000mL of distilled water, pH 7.3). On an air bath constant temperature oscillator, vibrate and cultivate for 24 hours under the conditions of a shaker speed of 220r / min and 28°C to form a seed solution; then inoculate the seed solution in a 50mL fermentation medium ( Rice flour 100g, bean cake powder 22g, yeast paste 5g, K 2 HPO 4 1g, NaCl 1g, CaCO 3 4g and 1000mL of distilled water, pH 7.5) in a 500mL Erlenmeyer flask, placed on an air bath constant temperature oscillator, and shaken at a shaker speed of 220r / min and 28°C for 4 days to obtain a fermentation broth.

[0020] Crude purification of flavonoids:

[0021] Use...

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Abstract

The invention relates to method for obtaining a flavonoid compound produced by streptomyces, which is prepared according to the following steps of: carrying out slant, seed and shaking-flask fermentation culture on streptomyces herbaricolor HNS2-2, carrying out acidification processing on fermentation liquid, centrifuging, extracting, concentrating and then carrying out silica gel column chromatography and thin-platechromatography on an extract phase to obtain three compounds which are verified as the flavonoid compounds. The flavonoid compound has wide biological activity, i.e. oxidation resistance, mutation resistance, ageing resistance, tumor resistance and the like, and has low toxicity, less adverse reaction and inhabiting and killing actions for microbes.

Description

Technical field: [0001] The invention relates to a flavonoid compound isolated from metabolites of soil streptomyces (streptomyces viridans HNS2-2). Background technique: [0002] Flavonoids (also known as flavonoids), mainly refers to the general term of a class of compounds with chromone ring and benzene ring as the basic structure. It is an important physiologically active substance with strong anti-oxidation, anti-radiation, anti- It has anti-cancer and anti-tumor effects, and has an inhibitory and killing effect on microorganisms. It has broad application prospects in the medical field and food industry. Flavonoids are widely found in plants in nature. Polyphenols and almost all plants contain a certain amount of flavonoids in flowers, leaves and stems. However, due to the seasonal influence of plant resources, the complex structure of flavonoids The difficulty of extraction and purification from plants, the unstable antibacterial effect of crude extracts of plant flav...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/06C12R1/465
Inventor 陈力力高必达李有志
Owner HUNAN AGRICULTURAL UNIV