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Magnetic nano lamellar hydroxyapatite and DNA composite as well as preparation method and application thereof

A hydroxyapatite and magnetic nanotechnology, applied in the field of biomedical materials, can solve problems such as the research of flaky hydroxyapatite without a magnetic layer, and achieve the effects of excellent superparamagnetic properties, easy preparation method and low cost

Active Publication Date: 2011-01-05
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, no research has been done on the adsorption, intercalation and enzymatic resistance of magnetically layered hydroxyapatite.

Method used

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  • Magnetic nano lamellar hydroxyapatite and DNA composite as well as preparation method and application thereof
  • Magnetic nano lamellar hydroxyapatite and DNA composite as well as preparation method and application thereof
  • Magnetic nano lamellar hydroxyapatite and DNA composite as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Add 5 mg of magnetic layer flaky hydroxyapatite powder into a conical flask (see CN 101759170A for the preparation method), and add a small amount of sterilized high-purity water. Intermittently ultrasonicated 3 times, each time for 1 min, ultrasonic power 100W, ultrasonic frequency: 40kHz, and finally a uniformly dispersed magnetic layer flaky hydroxyapatite suspension was obtained. Prepare a solution with a concentration of 1 mg / mL with sterilized high-purity water. Add 1mg of DNA solution dropwise into the Erlenmeyer flask and shake. Finally, adjust the volume to 20mL. Put the Erlenmeyer flask into a constant temperature shaker to vibrate, temperature: 37°C, rotation speed: 150rpm, and equilibrate adsorption for 0.5h. The obtained product was washed and ultracentrifuged at a speed of 10000 rpm, and dried at room temperature. Carry out XRD test, electrophoresis test and ultraviolet spectroscopic test, figure 1 is the X-ray small-angle diffraction pattern, figure...

Embodiment 2

[0019] Add 200mg of magnetic layer flaky hydroxyapatite powder into a conical flask (see CN101759170 for the preparation method), and add a small amount of sterilized high-purity water. Intermittently ultrasonicated 3 times, each time for 1 min, ultrasonic power 100W, ultrasonic frequency: 40kHz, and finally a uniformly dispersed magnetic layer flaky hydroxyapatite suspension was obtained. Prepare a solution with a concentration of 1 mg / mL with sterilized high-purity water. Add 1mg of DNA solution dropwise into the Erlenmeyer flask and shake. Finally, adjust the volume to 20mL. Put the Erlenmeyer flask into a constant temperature shaker to vibrate, temperature: 37°C, rotation speed: 150rpm, and equilibrate adsorption for 3.5h. The obtained product was washed and ultracentrifuged at a speed of 10000 rpm, and dried at room temperature. Carry out XRD test, electrophoresis test and ultraviolet spectroscopic test, figure 1 is the X-ray small-angle diffraction pattern, figure ...

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PUM

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Abstract

The invention relates to a magnetic nano lamellar hydroxyapatite and DNA composite and a preparation method thereof. Magnetic nano lamellar hydroxyapatite and salmon sperm DNA, used as raw materials, are blended according to the mass ratio of the DNA to the magnetic lamellar hydroxyapatite of 1:(5-200). The preparation method comprises the following steps of: shaking the suspension of the magnetic lamellar hydroxyapatite and a DNA solution at 37 DEG C for reacting for 0.5-3.5 hours; and after centrifuging at high speed, drying at normal temperature. The composite provided by the invention has an inserting and stripping type structure and realizes the bulk storage and effective protection of superhelix structure DNA. The preparation method of the composite is easier and has low cost and superparamagnetism. The invention has great significance for the magnetic lamellar hydroxyapatite in aspects of polygene medicament treatment and protein / gene composite medicament treatment.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and relates to a composite of magnetic nano-layer hydroxyapatite and DNA and its preparation method and application, in particular to a magnetic-layer flaky hydroxyapatite and DNA nano-gene drug carrier material preparation. Background technique [0002] Gene therapy is a new treatment technology that introduces human normal genes or genes with therapeutic effects into human target cells in a certain way to correct gene defects or exert therapeutic effects, so as to achieve the purpose of treating diseases. Appropriate gene vectors can safely, efficiently, controllably, and easily introduce target genes into target cells to realize their expression, so as to achieve the purpose of gene transfection or disease treatment. More than two-thirds of current gene therapy clinical trials use viruses as vectors. However, the disadvantages of viral vectors such as high immunogenicity, high toxicity, s...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K47/04
Inventor 万怡灶左桂福张宇
Owner TIANJIN UNIV
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