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Processing method of waste tobacco

A processing method and technology of waste tobacco leaves, applied in the field of tobacco processing, can solve the problem of low extraction efficiency of active ingredients, achieve the effects of rich active ingredients, increased pleasure, and reduced production costs

Active Publication Date: 2011-01-12
CHINA TOBACCO GUANGDONG IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the problem of low extraction efficiency of effective components in waste tobacco leaves, the present invention provides a processing method of waste tobacco leaves, which greatly improves the extraction efficiency of volatile components in waste tobacco leaves

Method used

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  • Processing method of waste tobacco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: the processing method of the present invention handles waste inferior tobacco leaves

[0030] The raw material waste tobacco leaves were hand-cut into shreds, dried in an oven at 40°C for 5 hours, crushed, left overnight, and stored in sealed bags for later use.

[0031] Take 30g of shredded tobacco leaves and place in a 500mL conical flask, add 300mL of citric acid-sodium citrate buffer solution with a pH value of 5.4. Then add 0.04g compound plant hydrolase, 0.08g cellulase, and 0.04g neutral protease. The above three enzymes were all purchased from Novozymes (China) Biotechnology Co., Ltd. 45°C air-bath shaker shake reaction for 5h, filter, add 100mL of water, shake and extract at room temperature for 20min, combine the extracts, and concentrate to 25mL by rotary evaporation under reduced pressure to obtain a concentrated extract.

Embodiment 2

[0032] Embodiment 2: processing method of the present invention handles waste inferior tobacco leaf

[0033] The raw material waste tobacco leaves were hand-cut into shreds, dried in an oven at 40°C for 5 hours, crushed, left overnight, and stored in sealed bags for later use.

[0034] Take 40g of shredded tobacco leaves and place in a 500mL conical flask, add 200mL of disodium hydrogen phosphate-potassium dihydrogen phosphate buffer solution with a pH value of 4.6. Then add 0.02 g of compound plant hydrolase Viscozyme L, 0.04 g of cellulase (fungal cellulase), and 0.02 g of neutral protease. The above three enzymes were all purchased from Novozymes (China) Biotechnology Co., Ltd. 50°C air-bath shaker shake reaction for 7h, filter, add 80mL of water, shake and extract at room temperature for 40min, combine the extracts, and concentrate to 25mL by rotary evaporation under reduced pressure to obtain a concentrated extract.

Embodiment 3

[0035] Embodiment 3: processing method of the present invention handles waste inferior tobacco leaf

[0036] The raw material waste tobacco leaves were hand-cut into shreds, dried in an oven at 40°C for 5 hours, crushed, left overnight, and stored in sealed bags for later use.

[0037] Take 20g of shredded tobacco leaves and place in a 500mL conical flask, add 160mL of acetic acid-sodium acetate buffer solution with a pH value of 4.8. Then add 0.04g compound plant hydrolytic enzyme, 0.08g cellulase, 0.04g neutral protease, 30°C air bath shaker shake reaction for 8h, filter, add 60mL water, shake and extract at room temperature for 60min, combine the extracts, and rotary evaporate under reduced pressure Concentrate to 25 mL to obtain a concentrated extract.

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Abstract

The invention relates to the field of processing of tobacco, and discloses a processing method of waste tobacco. The method comprises the following steps: reacting the waste tobacco, composite plant hydrolase accounting for 0.05-3 wt% of waste tobacco, 0.05-3 wt% of cellulase and 0.05-3 wt% of neutral proteinase in a buffer solution with the pH value of 4.5-6.0 for 4-8 hours, wherein the volume-to-mass ratio by mL / g of buffer solution to waste tobacco is 5-10:1; filtering after the reaction, taking the filter residues, and adding water for reextraction, wherein the volume-to-mass ratio by mL / g of water to waste tobacco is 2-4:1; and mixing the extracts. When the extraction yield and extract purity in the method and prior extraction method are measured, the result indicates that the method enhances the extraction efficiency by 25.2%, increases the content of volatile components by 74.2% as compared with the conventional method, and increases the content of main fragrance component neophytadiene by 6 times. The method has the advantages of low water consumption, low reaction temperature and simple operation, and lowers the production cost.

Description

technical field [0001] The invention relates to the field of tobacco processing, in particular to a method for processing waste tobacco leaves. Background technique [0002] The planting area of ​​tobacco plants in my country ranks first in the world, and the tobacco planting and product processing industry plays an important role in the development of the national economy. At present, tobacco grown at home and abroad is mainly used to make cigarette products. As a big tobacco country, in the process of growing and purchasing tobacco for cigarettes, there are about 200,000 tons of waste tobacco leaves in the country every year, accounting for about 13% of the total tobacco output in the country. These waste leaves are treated as waste, causing waste and pollution. , the foreign tobacco industry is facing the same situation. [0003] The disorderly flow of unused waste tobacco leaves has brought hidden dangers to the normal tobacco leaf market and caused a lot of waste of r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A24B3/14
Inventor 周瑢孔浩辉胡静李峰韩冰
Owner CHINA TOBACCO GUANGDONG IND
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