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Corn bran active polysaccharide and preparation method thereof

A technology of active polysaccharide and corn husk, applied in the field of deep processing of agricultural and sideline products and comprehensive utilization of by-products, can solve the problems of low economic benefit, low added value, poor market competitiveness, etc., and achieves a simple process route and less equipment investment. Effect

Inactive Publication Date: 2012-06-27
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Corn is the second largest grain variety in my country. Corn processing enterprises produce a large number of by-products—corn husks every year. Most of them are directly used as feed, which are not fully utilized and the added value is very low, resulting in high cost of the main product and market competition. Poor power, low economic benefit

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Finely sieved, cleaned, dried, and crushed corn bran raw materials to 60 mesh are mixed according to the material-liquid ratio of corn bran to water (weight to volume ratio v) 1:20, and then placed in a microwave processing device And control the microwave treatment power to be 200W, the microwave treatment time is 15min, and then put it in the ultrasonic device quickly, control the ultrasonic power to be 800W, after the ultrasonic time is 5min, carry out solid-liquid separation (rotating speed 4000r / min, centrifugation time 5min), discard Precipitate, retaining the supernatant is the phase extract of corn bran polysaccharides; using the Sevage method to remove protein, adding 1 / 8 volume of Sevage reagent (chloroform: n-butyl Alcohol=4:1), after shaking for 50min, let it stand for 100min, then centrifuge to remove the precipitated substance (rotating speed 10000r / min, centrifugation time 5min), select 5.0% activated carbon (powder), decolorization temperature 80 ℃, decol...

Embodiment 2

[0029] Finely sieved, cleaned, dried, and crushed corn bran raw materials to 60 mesh according to the material-liquid ratio of corn bran to water (weight to volume ratio v) 1:20, mixed until uniform, then placed in a microwave processing device And control the microwave treatment power to be 500W, the microwave treatment time is 3min, and then place it in the ultrasonic device quickly, control the ultrasonic power to be 200W, after the ultrasonic time is 5min, carry out solid-liquid separation (rotating speed 4000r / min, centrifugation time 30min), discard Precipitate, retaining the supernatant is the crude extract of corn bran polysaccharides; using the Sevage method to remove protein, adding 1 / 5 volume of Sevage reagent (chloroform: n-butyl Alcohol=4:1), after shaking for 10min, let it stand for 120min, then centrifuge to remove the precipitated substance (rotating speed 4000r / min, centrifugation time 30min), select 3.5% activated carbon (cylindrical), decolorization temperatu...

Embodiment 3

[0031]Finely sieved, cleaned, dried, and crushed corn bran raw materials to 60 mesh are mixed according to the material-liquid ratio of corn bran to water (weight to volume ratio v) 1:20, and then placed in a microwave processing device And control the microwave treatment power to be 350W, the microwave treatment time is 5min, then place it in the ultrasonic device quickly, control the ultrasonic power to be 100W, after the ultrasonic time is 35min, carry out solid-liquid separation (rotating speed 10000r / min, centrifugation time 5min), discard Precipitate, retaining the supernatant is the crude extract of corn bran polysaccharides; using the Sevage method to remove protein, adding 1 / 2 volume of Sevage reagent (chloroform: n-butyl Alcohol=4:1), after shaking for 20min, let it stand for 30min, then centrifuge to remove the precipitated substance (speed of rotation 8000r / min, centrifugation time 15min), select 0.5% activated carbon (granule), decolorization temperature 100 ℃, dec...

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Abstract

The invention relates to a method for extracting polysaccharides by comprehensively utilizing a corn non-starch component, i.e., corn bran, which belongs to the technical field of deep processing of agricultural byproducts and comprehensive utilization of by-products thereof. The invention discloses a corn bran active polysaccharide component and a preparation method thereof. The preparation method comprises the following steps of: taking the corn bran as materials to prepare the corn bran active polysaccharide component through the processes of microwave pretreatment, ultrasonic extraction, protein removing, bleaching, Sephadex G-75 column chromatography, concentration, and the like, wherein the extraction rate of a corn bran polysaccharide compound reaches as much as 8.16%, the protein removing rate reaches 80.42%, the sugar retention rate is 89.93 %, and the inhibition rate on S180 cells reaches as much as 59.12% which is 1.431 times as the value-added inhibition rate of 0. 05mg / mlcyclophosphamide. Therefore, a polysaccharides function factor component with antitumor activity developed by the invention not only can greatly improve the technical level of research and development on the corn non-starch component and the economic efficiency of corn processing enterprises, but also can provide an effective way for the development and the industrial transformation of corn bran active polysaccharide products.

Description

technical field [0001] The invention relates to a method for extracting active polysaccharides through the comprehensive utilization of corn husks, a non-starch component of corn, and belongs to the technical field of deep processing of agricultural byproducts and comprehensive utilization of byproducts. Background technique [0002] Corn is the second largest grain variety in my country. Corn processing enterprises produce a large number of by-products—corn husks every year. Most of them are directly used as feed, which are not fully utilized and the added value is very low, resulting in high cost of the main product and market competition. Poor power, low economic benefit. Plant polysaccharides have attracted people's attention due to their various physiological functions, such as anti-tumor, anti-infection, anti-radiation, and lowering blood sugar and blood lipids. If corn husks, a by-product of corn deep processing, can be further used to develop polysaccharide functiona...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C08L5/00
Inventor 林松毅王琴刘静波宫新统
Owner JILIN UNIV
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