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Method for extracting antifungal active substances of Streptomyces hygroscopicus

A technology of Streptomyces hygroscopicus and active substances, applied in the field of biopharmaceuticals, can solve the problems of unsatisfactory detoxification effect, incomplete detoxification, and destruction of nutrition, etc., and achieve suitable for large-scale production, excellent fermentation properties, and high antibacterial activity Effect

Active Publication Date: 2012-10-24
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods have defects such as destruction of nutrition, incomplete detoxification, and high cost, resulting in unsatisfactory effects of actual prevention and control and detoxification

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Activation of BS-112 strain and preparation of seed liquid: transfer the BS-112 strain preserved on the slope to a PDA medium plate, and cultivate it at 28°C for 96 hours; wash four 250mL triangular flasks, and put seeds in each bottle The volume of liquid medium is 50mL, and it is autoclaved; 2 pieces of activated BS-112 strains are inserted into the seed liquid medium, and cultured on a shaking table. Seed liquid culture conditions: temperature 28°C, shaker speed 200 rpm, culture time 48 hours;

[0025] (2) Fermentation of BS-112 bacterial strain: 250mL triangular flasks are cleaned, the quantity is 60, and the amount of fermented liquid in each bottle is 50mL, and autoclaved; the seed liquid is inoculated in the fermentation medium by 2% inoculation amount, Shake flask fermentation, the fermentation conditions are: temperature 28°C, shaker speed 200 rpm, culture time 84 hours. After the fermentation, collect the fermented liquid, centrifuge at 4200 r / min in a lo...

Embodiment 2

[0030] (1) Activation of BS-112 strain and preparation of seed liquid: transfer the BS-112 strain preserved on the inclined plane to a PDA medium plate, and cultivate it at 28°C for 96 hours; wash two 500mL triangular flasks, and put seeds in each bottle The volume of the liquid culture medium is 110mL, and it is sterilized under high pressure; 3 blocks of activated BS-112 strains are inserted into the seed liquid medium, and cultured on a shaking table. Seed liquid culture conditions: temperature 28°C, shaker speed 200 rpm, culture time 48 hours;

[0031] (2) Fermentation of BS-112 bacterial strain: 500mL triangular flasks are cleaned, the quantity is 50, and the amount of fermented liquid in each bottle is 110mL, and autoclaved; the seed liquid is inoculated in the fermentation medium by 2% inoculation amount, Shake flask fermentation, the fermentation conditions are: temperature 28°C, shaker speed 200 rpm, culture time 96 hours. After the fermentation is over, collect the ...

Embodiment 3

[0036] (1) Activation of BS-112 strain and preparation of seed solution: transfer the BS-112 strain preserved on the slope to a PDA medium plate, and cultivate it at 28°C for 72 hours; wash four 250mL triangular flasks, and put seeds in each bottle The amount of liquid medium is 50mL, autoclaved; insert 2 blocks of activated BS-112 strains into the seed liquid medium, and cultivate on a shaking table. The seed liquid culture conditions: temperature 28°C, shaking table speed 200 rpm , culture time 36 hours;

[0037] (2) Fermentation of BS-112 bacterial strain: 250mL triangular flasks are cleaned, the quantity is 60, the amount of fermented liquid in each bottle is 55mL, and autoclaved; the seed liquid is inoculated in the fermentation medium by 3% inoculation amount, Shake flask fermentation, the fermentation conditions are: temperature 28°C, shaker speed 200 rpm, culture time 84 hours. After the fermentation, collect the fermented liquid, centrifuge in a low-speed large-capac...

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Abstract

The invention relates to a method for extracting antifungal active substances of Streptomyces hygroscopicus, which comprises the following steps of: 1, fermenting the Streptomyces hygroscopicus BS-112 (Streptomyces hygroscopicus BS-112)(CGMCC No.3504) to obtain fermentation broth; 2, adsorbing supernatant after centrifuging the fermentation broth by using X-5 resin; 3, performing desorption on the antifungal active substances by using 70-80% ethanol; and 4, collecting desorption solution, and performing depressurized evaporative concentration and freeze-drying on the desorption solution to obtain an extract of the antifungal active substances. The method for extracting the antifungal active substances of the Streptomyces hygroscopicus BS-112 is simple and convenient, has a short production cycle and a low cast, and is suitable for large-scale production. The antifungal active substances obtained by the method have broad-spectrum antibacterial activity to main moldy fungi and various bombyx mori and plant pathogenic fungi in foodstuffs and feeds, are low in toxicity, and have high practical application value and extensive market prospect on biological control of foodstuff and feed preservatives, bombyx mori fungal diseases and plant fungal diseases.

Description

technical field [0001] The invention relates to a method for extracting antifungal active substances of Streptomyces hygroscopicus. It belongs to the technical field of biopharmaceuticals. Background technique [0002] The purpose of the present invention is to extract the antifungal active substance produced by the fermentation of Streptomyces hygroscopicus BS-112 and use it to prevent and control main mildew fungi such as Aspergillus flavus, Aspergillus ochraceae and Aspergillus niger in grain and feed. [0003] Mycotoxins are secondary toxic metabolites produced by fungi. They exist in the mycelia and spores of filamentous fungi and widely contaminate grain, food and feed. According to the estimates of the Food and Agriculture Organization of the United Nations, at least 25% of the world's grains are currently contaminated by mycotoxins, causing economic losses of up to hundreds of billions of dollars every year. my country is the hardest-hit area of ​​mycotoxins, and t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/06B01D15/02C12R1/55
Inventor 刘训理张楠毛志泉宋振张本峰于建国辉仇念全
Owner SHANDONG AGRICULTURAL UNIVERSITY
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