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Method for preparing antibacterial compound

A compound and seed technology, applied in the field of bioengineering, can solve problems such as no treatment methods, and achieve the effects of easy separation and purification, high potency, and simple process flow

Inactive Publication Date: 2013-04-17
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, topical therapy has been able to cure most patients with superficial mycosis, but there are still few drugs that can treat deep mycosis, and some diseases still have no effective treatment

Method used

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  • Method for preparing antibacterial compound
  • Method for preparing antibacterial compound
  • Method for preparing antibacterial compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0033] Medium

[0034] (1) LB slant medium (%): yeast powder 0.5, peptone 1.0, NaCl 0.5, agar 2.0, adjusted to pH 7.4-7.6.

[0035] (2) Seed medium (%): glucose 1.5, yeast powder 1.0, K 2 HPO 4 0.1, MgSO 4 0.05, FeSO 4 0.001, add CaCO after adjusting pH 7.0 3 0.04.

[0036] (3) Fermentation medium (%): soybean powder 0.6, soluble starch 3.0, yeast powder 1.0, K 2 HPO 4 0.1, NaCl 0.2, MgSO 4 0.005, FeSO 4 0.002, pH 6.5.

[0037] (4) Candida albicans culture medium (%): glucose 2.0, peptone 1.0, agar 2.0.

[0038] Strain isolation and purification

[0039] Five-point cross-sampling of soil samples in the area delineated by the East China Sea in Zhejiang Province. Take 10g of soil sample at each point and put it in an Erlenmeyer flask. After mixing, take 10g of soil sample and add it to a cone filled with 90mL of sterile water. In a bottle, place it on a shaker and shake for 30 minutes to fully disperse the bacteria in the soil sample in water, which is 10 -1 Bacteria. After the ino...

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Abstract

The invention discloses a method for preparing an antibacterial compound, which comprises the following steps of: inoculating bacillus subtilis (Bacillus subtilis) GMCC No.4140 into a fermentation medium, culturing for 12 to 60 hours, separating and purifying. The antibacterial compound has strong effect of inhibiting Candida albicans and better antibacterial effect than the conventional nystatin. The antibacterial compound has strong effect of inhibiting the Candida albicans and other yeast fungi, also has good effect on Aspergillus niger and other filamentous fungi, and simultaneously has better effect of inhibiting Escherichia coli and other bacteria. The antibacterial compound produced by the method has high potency, the technical flow is simple, and antibacterial substances are easy to separate and purify.

Description

Technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for preparing an antibacterial compound. Background technique [0002] In recent years, with the improvement of modern medical treatment, the survival rate of cancer, AIDS, organ and bone marrow transplantation patients has greatly increased. However, these patients often use high-dose antibiotics, anti-tumor drugs, hormones, immunosuppressants and other drugs, and Chemotherapy, intravenous intubation, hemodialysis and other interventional treatments cause a decrease in immunity, and it is very easy to be invaded by pathogenic fungi that are not pathogenic and cause systemic fungal infection. In recent years, this serious life-threatening fungal infection has been increasing rapidly, and the number of patients has been increasing. [0003] Clinically, with the increase in the rate of fungal infection, the use rate of antifungal drugs has increased, and subsequently drug...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/08C12N1/20C12R1/125
Inventor 吴绵斌张书衍贺娟陈正杰
Owner ZHEJIANG UNIV
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