Method for screening high-lipid content mutant microalgae strain

A technology of mutant strain and oil content, applied in the field of microbial engineering, can solve the problems of restricting the development and utilization of biodiesel, time-consuming and labor-intensive mutant strains, etc., and achieves good development prospects, reducing waste of raw materials, and high oil content.

Inactive Publication Date: 2011-03-23
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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Problems solved by technology

There have been successful examples of using ultraviolet rays to mutagenize algal strains in microalgae, but the screening of mutant st

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  • Method for screening high-lipid content mutant microalgae strain
  • Method for screening high-lipid content mutant microalgae strain
  • Method for screening high-lipid content mutant microalgae strain

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Embodiment Construction

[0024] Below in conjunction with example the present invention is described in further detail.

[0025] In the first step, after treating the chlorella cells with ultraviolet rays, the color of the algae liquid becomes lighter from the original green. Observed under the microscope, the number of surviving cells decreased with the prolongation of irradiation time. When the death rate of algae cells was stable on the 3rd day after irradiation, the death rate was counted (see figure 1 , figure 2 ). Various ultraviolet doses used in the experiment have a lethal effect on Chlorella cells, and the semi-lethal dose is IC 50 = 10 min. In order to obtain more mutant strains without causing most of the cells to die due to excessive radiation dose and leave some inactive cells, an 18W UV lamp was used, the irradiation distance was 40cm, and the irradiation time was 13min, which was suitable for this experiment.

[0026] The second step, ultraviolet mutagenesis to obtain mutant str...

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Abstract

The invention relates to a method for screening a high-lipid content mutant microalgae strain, which comprises the following steps of: performing irradiation treatment on the diluted algae solution of chlorella vulgaris Y019; performing dark culture on mutagenic algae solution, coating the cultured algae solution onto a solid high salt medium (HSM) for culture, sequentially inoculating the cultured algae solution onto a new solid HSM, performing numbering and testing OD490; adding a Nile red dye and dimethylsulfoxide (DMSO) into the algae solution, and testing the lipid content of the mutant microalgae strain; and when the ratio C of a unit OD490 neutral lipid absorption value B1 of the mutant strain to the unit OD490 neutral lipid absorption value B2 of the chlorella vulgaris Y019 is over 110 percent, determining the mutant strain as the high-lipid content mutant strain which is named Y019-M37 and preserved at the China center for type culture collection with the collection number of CCTCC NO.M2010170. The method is simple to screen and detection and consumes less time; and the obtained mutant strain has a high growth rate and high lipid content, is a useful source for the preparation of biodiesel and has vast biodiesel development prospect.

Description

technical field [0001] The invention relates to the field of microbial engineering, and relates to a method for screening microalgae with high oil content by ultraviolet mutagenesis, in particular to a high-efficiency screening method for mutant strains with high oil content produced by ultraviolet mutagenesis of chlorella. Background technique [0002] With the continuous development of global economic integration, the world's energy situation is becoming increasingly tense. The extensive use of petroleum fuels has made the energy crisis and environmental problems increasingly prominent. The continued use of petroleum fuels is widely considered unsustainable. Looking for a green sustainable development New energy has become a concern of all countries. Biodiesel is a green and sustainable new energy source researched by scientists. It mainly comes from: oily plants, waste oil and animal fat, and the biodiesel produced from these sources is far from meeting the current fuel n...

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Application Information

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IPC IPC(8): C12N1/12C12N13/00C12N15/01C12R1/89
Inventor 邓晓东费小雯周玉娇
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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