Synthetic peptide vaccine for swine fever and application thereof

A technique for synthesizing peptide vaccines and swine fever, applied to non-active ingredient medical preparations, active ingredient-containing medical preparations, peptides, etc., can solve problems such as inability to effectively protect pigs, achieve easy control of production quality, and improve immunity The effect of originality and stable quality

Inactive Publication Date: 2013-03-27
SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using the method of synthesizing overlapping peptides, a series of linear neutralizing epitopes of classical swine fever E2 protein have been identified. After coupling the above epitopes with BSA, immunizing pigs can induce neutralizing antibodies, but cannot Pigs provide complete protection (Dong et al., Vaccine, 2006, 24:1906-1913; Dong et al., Vaccine, 2006, 24:426-434)
Neither the fusion expression of the epitope and GST nor the coupling of the synthetic peptide chain and BSA in the above studies can effectively protect pigs, and the immune response against the vector will inevitably be generated after immunization of animals, which may bring serious symptoms to the immunized animals. come at a certain risk

Method used

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  • Synthetic peptide vaccine for swine fever and application thereof
  • Synthetic peptide vaccine for swine fever and application thereof
  • Synthetic peptide vaccine for swine fever and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] The branched peptides and linear peptides shown below were artificially synthesized by conventional methods in the art, and named E28 and E26, respectively. The purity of the synthetic peptides is above 90%. Dilute the synthetic peptides with sterile PBS to a peptide solution with a concentration of 1 microgram per microliter, and then mix the peptide solution with an equal amount of protein adjuvant (such as Freund's adjuvant, etc.) Mixed, emulsified into a vaccine, used as an immunogen for immunizing animals.

[0059] Branched Peptide-E28

[0060] Linear peptide - E26ISISEIKGVIVHKIEGILFKKCTAVSPTTLRTEVVK

[0061] Select 15 healthy rabbits weighing about 2 kg, and divide them into 3 groups, 5 rabbits in each group. Rabbits No. 8, 9, and 10) were immunized with branched peptide E28, and the third group (rabbits No. 11, 12, 13, 14, and 15) were immunized with PBS as a negative control. The peptide vaccine immunization dose is 50 micrograms each time, with a total vol...

Embodiment 2

[0067] Peptide vaccines were prepared as in Example 1 and used as immunogens to immunize animals. Select 20 healthy piglets negative for swine fever antibody about 35 days old and divide them into 4 groups, 5 pigs in each group. The first group is immune to the linear peptide E26, the second group is immune to the branched peptide E28 prepared by the present invention, and the remaining 2 groups are respectively Immune attenuated classical swine fever vaccine (HCLV) and PBS, as positive control and negative control. The dose of peptide vaccine immunization is 300 micrograms each time, the total volume is 1 ml, multi-point intramuscular injection, immunization 2 times, with an interval of 2 weeks. The first emulsification with Freund's complete adjuvant and the second emulsification with Freund's incomplete adjuvant. Before immunization and after immunization, blood was collected every week to separate serum, and the serum antibody level was detected by peptide ELISA and IDEXX...

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Abstract

The invention discloses a synthetic peptide vaccine for swine fever. The synthetic peptide vaccine for the swine fever is characterized in that an auxiliary T cell epitope is connected with a swine fever virus E2 protein B cell epitope by using a branch peptide. The synthetic peptide vaccine for the swine fever not only prevents the organisms from generating immune responses aiming at carriers, but also enhances the immunogenicity of the synthetic peptide vaccine, has pure component without toxic side effect or biosafety aspect worries and industrialization superiority.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a synthetic peptide vaccine for swine fever and its application. Background technique [0002] The E2 protein of CSFV can induce the production of neutralizing antibodies and is the main protective antigen of CSFV (Konig et al., JVirol, 1995, 69:479-86). Some progress has been made in the identification of antigenic epitopes of CSFV E2 protein. Among them, amino acids 829-837 of E2 protein are a major antigenic epitope, and it is a conserved classical classical classical epitope (Lin et al., J Virol, 2000, 74: 11619-25). The fusion product of this epitope and GST expressed in Escherichia coli induced a protective immune response (Liu et al., J Virol Meth, 2006, 134:125-9), and after tandem 4 copies, the expressed fusion protein could resist pig Pestivirus challenge (Liu et al., Vaccine, 2006, 24:7175-7180). After coupling the artificially synthesized peptide chain of th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/187A61K47/48C07K7/06A61P31/20A61K47/65
Inventor 童光志李国新周艳君于海
Owner SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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