Pichia pastoris, fermenting cultivating method thereof, fungicide, preparing method thereof and application

A Pichia pastoris, fermentation culture technology, applied in the field of microorganisms, can solve the problems of pathogenic bacteria resistance, increased drug dosage, increased pesticide residues on fruits and vegetables, etc., and achieves a simple preparation process, broad application prospects, and short fermentation cycles. Effect

Inactive Publication Date: 2011-04-20
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term use of fungicides can easily lead to the emergence of pathogenic bacteria resistance, and the continuous increase in the dosage of fungicides will increase the amount of pesticide residues on fruits and vegetables, directly threatening human health. This has become one of the food safety issues that the public is most concerned about today.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The formula of PDA culture medium is: every 1000mL water contains potato 200g, glucose 20g, agar 15g, pH 6.0. The formula of the PDA culture solution is without adding agar, and the others are the same as above.

[0022] The formula of Pichia pastoris (P. pastoris) CH01 special fermentation medium is: every 1000mL of water contains 5g of corn flour, 5g of bean cake powder, 5g of peanut powder, KH 2 PO 4 2.5g, MgSO 4 0.25g, CaCl 2 0.25g, urea 0.24g, brown sugar 5g, pH 5.5.

Embodiment 2

[0023] Example 2, Preservation of Pichia pastoris (P.pastoris) CH01 and its fermentation culture

[0024] 1. Preservation of strains

[0025] Pichia pastoris (P.pastoris) CH01 was isolated from the surface of muskmelon in Inner Mongolia Autonomous Region, and was preserved in the General Microbiology Center of China Committee for the Collection of Microorganisms on November 1, 2010. Address: No. 1 Beichen West Road, Chaoyang District, Beijing No. 3, Institute of Microbiology, Chinese Academy of Sciences, deposit number is CGMCC No.4273; reference biological material: CH01; classification name: Pichia pastoris.

[0026] 2. Fermentation of Pichia pastoris (P.pastoris) CH01

[0027] The fermentation culture method of Pichia pastoris (P.pastoris) CH01, comprises the following steps:

[0028] 1) Activation of strains

[0029] Pichia pastoris (P. pastoris) CH01 strain was inoculated into PDA medium, and cultured at 25° C. for 48 h.

[0030] 2) Seed tank culture

[0031] The act...

Embodiment 3

[0034] Embodiment 3, the test of detection Pichia pastoris (Pichia pastoris) CH01 on fruit and vegetable pre-harvest and post-harvest control effect

[0035] Taking Fusarium spp. of melon as an example

[0036] (1) Detect the field control effect of Pichia pastoris (P.pastoris) CH01 on post-harvest diseases of melon, the specific method is: the test sets two treatments as blank control and biocontrol Pichia pastoris (P.pastoris) CH01 deal with. A total of 6 plots were repeated 3 times, and the plots were randomly arranged, and each plot was guaranteed to have ten well-growing and robust melon plants. Spray the biocontrol Pichia pastoris (P. pastoris) CH01 bacterial solution at the flowering stage and fruit setting stage of melon respectively, and the concentration of biocontrol bacteria is 1×10 8 CFU / mL. After the melons are ripe, put them into cartons respectively and store them at room temperature. Results The control effect of melon treated with P. pastoris (P. pastoris...

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Abstract

The invention discloses a Pichia pastoris CH01, a fermenting cultivating method thereof and an application. The preservation number of the Pichia pastoris CH01 is CGMCC No.4273. The fermenting cultivating method of the Pichia pastoris CH01 comprises the steps of: 1) inoculating activated Pichia pastoris CH01 into the frequently used fermenting culture solution of the Pichia pastoris CH01 so as tocultivate seed solution and 2) inoculating the seed solution obtained in the step 1) to the frequently used fermentation medium of the Pichia pastoris CH01 so as to aerobically culture and obtain thePichia pastoris CH01 solution. The Pichia pastoris CH01 has short fermentation period, is simple in preparation process, and has reliable industrial production. The invention has wide application prospect in preventing and treating potato late blight and controlling the postharvest diseases of fresh fruit and vegetables.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Pichia pastoris and its fermentation culture method, bacterial agent and the production method of the bacterial agent, and also relates to the application of Pichia pastoris. Background technique [0002] There are some beneficial bacteria in the plant rhizosphere or itself, which can prevent and control plant diseases caused by pathogenic bacteria directly or indirectly. These microorganisms that can prevent diseases and increase crop yields are collectively called biocontrol bacteria. ). Biocontrol bacteria have a biological control effect on harmful pathogenic microorganisms in the soil and non-parasitic rhizosphere harmful microorganisms. Biocontrol bacteria have the advantages of good antagonistic effect, no toxins, and can be used together with chemical fungicides, so they have become a hot spot in disease control research. [0003] At present, chemical...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16A01N63/04C12R1/84
Inventor 周洪友宋娟李小娟杨继峰张一名王丹
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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