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Bacillus thuringiensis wettable powder containing pullulan polysaccharide and preparation method thereof

A technology of pullulan polysaccharide and thuringiensis spore, which is applied in the fields of botanical equipment and methods, pesticides, biocides, etc. High activity, good shelf life and lasting effect, make up for the effect of a single preparation process

Inactive Publication Date: 2011-04-27
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims to solve the problems that the main effective components of the existing Bacillus thuringiensis powder are not stable, the shelf life and duration of the active ingredients are poor, and the preparation process is single and obsolete, and provides a Bacillus thuringiensis containing pullulan polysaccharide that can Wet powder and preparation method thereof

Method used

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  • Bacillus thuringiensis wettable powder containing pullulan polysaccharide and preparation method thereof
  • Bacillus thuringiensis wettable powder containing pullulan polysaccharide and preparation method thereof
  • Bacillus thuringiensis wettable powder containing pullulan polysaccharide and preparation method thereof

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specific Embodiment approach 1

[0014] Specific embodiment one: In this embodiment, a Bacillus thuringiensis wettable powder containing pullulan consists of 50% to 70% of spore and paraspore crystal mixture, 0.5% to 5% of carrier, 5% to Composed of 20% wetting agent, 5% to 20% dispersant and 1% to 5% NaCl; wherein the ratio of spores to paraspore crystals in the mixture of spores and paraspore crystals is 0.8 to 1.2:1, and the dispersant For pullulan.

specific Embodiment approach 2

[0015] Embodiment 2: This embodiment is different from Embodiment 1 in that the carrier is artificial zeolite, kaolin or diatomaceous earth. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0016] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the wetting agent is sodium dodecylbenzenesulfonate or sodium dodecyl sulfate. Others are the same as in the first or second embodiment.

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Abstract

The invention provides bacillus thuringiensis wettable powder containing pullulan polysaccharide and a preparation method thereof, relating to powder and a preparation method thereof. The invention solves the problems that the quality guarantee period and the valid period of the powder active ingredients prepared by the traditional technology are short, and the preparation process is single and dated. The bacillus thuringiensis wettable powder containing pullulan polysaccharide of the invention comprises a spore and parasporal crystal mixture, a carrier, a wetting agent and a dispersing agent. The preparation method comprises the steps of: (1) preparing seed bacteria liquid; (2) fermenting and cultivating the seed bacteria liquid to obtain fermentation liquid; (3) concentrating the fermentation liquid to obtain spore and parasporal crystal; (4) adding the carrier and NaCl to the spore and parasporal crystal mixture and blending uniformly; and (5) adding the wetting agent and the dispersing agent, blending uniformly, drying and grinding to obtain the bacillus thuringiensis wettable powder containing pullulan polysaccharide. The bacillus thuringiensis in the powder obtained in the invention has high activity, long quality guarantee period and valid period, and can be widely applied to the field of pest control in the field.

Description

technical field [0001] The invention relates to a powder and a preparation method thereof. Background technique [0002] One of the most important causes of crop yield reduction today is agricultural insect pests. Although traditional chemical pesticides have a certain control effect on pests, their negative impacts are becoming more and more serious, making pests resistant to pesticides, reducing efficacy and increasing The cost of prevention and control is relatively high, and it is not only harmful to humans and animals, but also pollutes the environment, kills natural enemies, and even destroys the ecological balance. Microbial insecticides overcome the above shortcomings, and have the advantages of strong selectivity, safety, and simple raw materials, so they have become one of the research hotspots in crop pest control. Among the currently studied microbial insecticides, Bacillus thuringiensis (Bt) is the most researched and most favored biocontrol agent. [0003] Si...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01N25/14A01N25/10A01P7/04
Inventor 杨峰山韩德权刘春光李云龙朱勋
Owner HEILONGJIANG UNIV
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