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Artificially cultured Grifulaumbelleta strain, Amillariella mellea and sclerotium production method for Grifulaumbelleta strain

A production method, the technology of Armillaria armillaria, applied in botany equipment and methods, fertilizer mixtures, horticulture, etc., can solve the problems of restricting Polyporus umbilicalis, difficulties in artificial cultivation provenance, and rising artificial cultivation costs, so as to achieve rapid formation of sclerotia , to meet the effect of large-scale production and low production cost

Inactive Publication Date: 2011-05-04
LIAONING FORESTRY SCI RES INST
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  • Abstract
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AI Technical Summary

Problems solved by technology

Journal of Agricultural Sciences of Yanbian University, 2004, 24 (4): 241-244), lead to the difficulty of artificial cultivation provenance, cause artificial cultivation cost to rise, have become the main bottleneck that restricts the large-scale implementation of Polyporus artificial cultivation technology (document 4. Cheng Xian Good. Artificial cultivation of Polyporus and Armillaria armillaria and research on the factors affecting the production of several active ingredients

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1) Inoculate the strain of Polyporus spp. in a sterile environment, the volume ratio of the strain of Polyporus spp. to the culture medium is 25:100, cultivate it at 20°C for 20 days to make a first-class strain, and form a large number of sclerotia on the inclined surface of the test tube;

[0026] 2) After sterilizing with the formula of the primary medium, cool the upright test tube at 25°C, transfer Armillaria armillaria, the volume ratio of the strain to the medium is 25:100, and the culture conditions are the same as that of Polyporus sp., to obtain Armillaria armillaria kind;

[0027] 2. Prepare a secondary culture medium with a water content of 60% by weight ratio of 20% corn squid and 80% eucalyptus wood chips. The corn squid is pre-soaked in water for 12-16 hours before use; then put the secondary medium into Seal the jar with a polypropylene film and autoclave for 90 minutes at 121°C; cool down to below 25°C and inoculate the first-grade strains of Polyporus...

Embodiment 2

[0031] 1) Inoculate the strain of Polyporus spp. in a sterile environment, the volume ratio of the strain of Polyporus spp. to the culture medium is 20:100, cultivate it at 27°C for 20 days to make a first-grade strain, and form a large number of sclerotia on the inclined surface of the test tube;

[0032] 2) After sterilizing with the formula of the primary medium, cool the upright test tube at 25°C, transfer to Armillaria armillaria, the volume ratio of the strain to the medium is 20:100, and the culture conditions are the same as that of Polyporus sp.;

[0033]2. Prepare the secondary culture medium with 55% water content with 60% corn squid, 40% broad-leaved maple and elm with a diameter of 0.1-2cm and 3-5cm long small wood section. Soak in water for 1 hour to pre-wet; then put the secondary culture medium into a can bottle and seal it with a polypropylene film, and autoclave for 90 minutes at 121°C; cool down to below 25°C, and inoculate the pigs obtained in step "1" at th...

Embodiment 3

[0037] 1) Inoculate the strain of Polyporus spp. in a sterile environment, the volume ratio of the strain of Polyporus spp. to the culture medium is 16:100, cultivate it at 25°C for 18 days to make a first-class strain, and form a large number of sclerotia on the inclined surface of the test tube;

[0038] 2) After sterilizing with the first-grade medium formula, cool the upright test tube at 25°C, transfer to Armillaria armillaria, the volume ratio of strain to medium is 16:100, and the culture conditions are the same as that of Polyporus sp.;

[0039] 2. Prepare a secondary medium with a water content of 58% by weight ratio of 30% corn squash and 70% chestnut sawdust. Corn squash needs to be soaked in water and pre-wetted 12-16 hours in advance; then put the secondary medium into canned bottles And seal it with a polypropylene film, and sterilize under high pressure at 121°C for 90 minutes; after cooling to below 25°C, simultaneously inoculate the first-class strains of Polyp...

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Abstract

The invention relates to a traditional Chinese medicine of Grifulaumbelleta, in particular to an artificially cultured Grifulaumbelleta strain, Amillariella mellea and a sclerotium production method for the Grifulaumbelleta strain. The Grifulaumbelleta strain is Linyan Grifulaumbelleta NO.3 and the accompanying Amillariella mellea is Linyan No.3; and the sclerotium production method for the Grifulaumbelleta strain comprises the following steps of: preparing a first-degree culture medium from 10 to 50g of corn meal, 10 to 20g of saccharose or glucose, 1 to 3g of beef extract, 15 to 22g of agar and 1,000ml of water, sterilizing, inoculating the Grifulaumbelleta strain, and culturing to obtain a first-degree strain; and 2) mixing 20 to 60 weight percent of maize pulp and 80 to 40 percent of broad-leaved tree sawdust and / or small wood block with the diameter of 0.1 to 2cm and the length of 3 to 5cm to form a second-degree culture medium, adding water, stirring uniformly to ensure the water content of the culture medium is 55 to 60 percent, sterilizing, simultaneously inoculating the first-degree Grifulaumbelleta strain and the Amillariella mellea, and culturing at the temperature of between 20 and 25DEG C for 30 to 45 days to obtain a second-degree strain. The invention has the advantages of low production cost and quick sclerotium formation.

Description

technical field [0001] The invention relates to Chinese medicinal material Polyporus, in particular to a method for producing sclerotia of Polyporus artificially cultivated strain, associated Armillaria armillaria and Polyporus strain. Background technique [0002] Polyporus is a traditional rare Chinese medicinal material in my country. At present, Polyporus adopts wild sclerotium as a semi-artificial cultivation technique (document 1. Guo Shunxing, Xu Jintang, Xiao Peigen. Progress in Biological Research of Polyporus. Chinese Journal of Traditional Chinese Medicine, 1996, (9) :515-517). Due to the expansion of the pharmaceutical market and exports, wild product resources have declined sharply, making it difficult to meet market demand. Adopting the cultivation method of wild sclerotium as seed requires a large amount of wild sclerotia (the second year product of Polyporus sclerotium) (document 2. Xu Guangbo, Fu Weijie. Zhao Xukui, the progress of China's Polyporus scleroti...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G3/00
Inventor 金若忠范俊岗栾庆书云丽丽叶景丰陈罡马冬菁高军
Owner LIAONING FORESTRY SCI RES INST
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