Method for screening denitrifying bacterium

A technology for denitrifying bacteria and screening methods, which is applied in chemical instruments and methods, biochemical equipment and methods, and microbial determination/inspection, etc., which can solve the problem of insufficient total nitrogen removal capacity and no aerobic domestication process of high-concentration ammonia nitrogen sewage. and other problems, to achieve the effect of strong adaptability, good performance and wide adaptability

Active Publication Date: 2011-05-04
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
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AI Technical Summary

Problems solved by technology

The strains obtained by this method are enriched by the method of domesticating anaerobic denitrification sludge to enrich the biological flora, so that the screened strains come from an anaerobic environment and have not experienced the aerobic domestication process of high-concentration ammonia-nitrogen sewage. When impacting, the total nitrogen removal capacity is insufficient; this method requires the influent COD to be controlled within the range of 4500-5500mg / L, which is limited in the treatment of different sewage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022](1) Enrichment culture: Take a certain amount of activated sludge from the aerobic aeration tank of a refinery sewage treatment plant, put it into the aerobic reactor, and gradually increase the concentration of substrate ammonia nitrogen for enrichment. The concentration is 100mg / L, and the COD concentration is 350mg / L. During the cultivation process, when the ammonia nitrogen concentration is lower than 10mg / L, add nitrogen source to increase the ammonia nitrogen concentration by 100mg / L from the previous time until it reaches 1000mg / L. During enrichment: temperature is 25°C; pH 7.5, DO (dissolved oxygen) 2.5mg L -1 about.

[0023] (2) Screening and purification: Get a certain amount of mud-water mixture obtained in step (1) and dilute it in different multiples and spread it on a solid medium plate for cultivation. The nitrate nitrogen concentration in the culture solution is 100mg / L, and also contains a small amount of Fe 2+ , Mg 2+ 、K + , Ca 2+ Metal ions and ph...

Embodiment 2

[0027] (1) Enrichment culture: Take a certain amount of activated sludge from the aerobic aeration tank of a catalyst sewage treatment plant, put it into the aerobic reactor, and gradually increase the concentration of substrate ammonia nitrogen for enrichment. The concentration is 200mg / L, and the COD concentration is 200mg / L. When the ammonia nitrogen concentration is lower than 50mg / L during the cultivation process, the ammonia nitrogen concentration will be increased by 150mg / L from the previous time until it reaches 1500mg / L. During enrichment: temperature is 30°C, pH 7.8, DO (dissolved oxygen) 5mg L -1 about.

[0028] (2) Screening and purification: Take a certain amount of mud-water mixture and dilute it in different times and spread it on a solid medium plate for cultivation. The nitrate nitrogen concentration in the culture solution is 100mg / L, and it also contains a small amount of Fe 2+ , Mg 2+ 、K + , Ca 2+ Metal ions and phosphate ions, etc., were cultured at a...

Embodiment 3

[0032] Take a certain amount of activated sludge from the aerobic aeration tank of a refinery sewage treatment plant, access temperature: 28°C, pH: 8.0, DO: 4mg·L -1 The enrichment is carried out in the aerobic reactor. The initial ammonia nitrogen concentration of the sewage is 150mg / L, and the COD concentration is 300mg / L. L until it is increased to 1200mg / L.

[0033] Take a certain amount of the above-mentioned mud-water mixture and dilute it in different times and spread it on a solid medium plate for culture. The nitrate nitrogen concentration in the culture solution is 80mg / L, and it also contains a small amount of Fe 2+ , Mg 2+ 、K + , Ca 2+ Metal ions and phosphate ions, etc., were cultured at a constant temperature of 28°C, using sodium citrate as a carbon source, configured according to a carbon-to-nitrogen mass ratio of 12:1, and using bromothyme as an indicator for separation and purification. After culturing for 2-3 days, select a single colony that turns blue ...

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Abstract

The invention discloses a method for screening denitrifying bacterium. The method comprises the following steps: (1) performing accumulation culture process on aerobic activated sludge by using ammonia-containing sewage; (2) separating and purifying denitrifying bacterium from accumulated flora; (3) acclimatizing the growing ability of the purified denitrifying bacterium with the water containingtarget pollutant; and (4) acclimatizing the denitrification ability of the acclimatized denitrifying bacterium under different dissolved oxygen conditions. When the denitrifying bacterium screened byusing the method disclosed by the invention is applied to denitrification treatment of sewage, the denitrifying bacterium not only can overcome the shortcomings of the traditional biologic denitrification technology, but also can be replenished into the impacted sewage treatment system as bioremediation agent so as to quickly recover the treating capability of the system and provide the conditionof synchronous nitration and denitrification.

Description

technical field [0001] The invention belongs to the technical field of sewage biological treatment, and in particular relates to a method for screening high-efficiency aerobic denitrifying bacteria using nitrate nitrogen as the only nitrogen source. Background technique [0002] In the field of sewage treatment, biological denitrification is considered to be one of the cost-effective sewage denitrification methods. The traditional biological denitrification process regards nitrification and denitrification as two independent processes, which are carried out in different reactors, such as A / A / O, A / O, etc.; or use intermittent aerobic and anaerobic conditions to Operation, such as SBR, oxidation ditch, etc., that is, the nitrification reaction occurs under aerobic conditions, and the denitrification reaction occurs under strict anoxic or anaerobic conditions, so that the two are separated in time or space. This makes the process longer, the investment in infrastructure is hig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C02F3/34
Inventor 高会杰黎元生李志瑞唐似茵
Owner CHINA PETROLEUM & CHEM CORP
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