Transgenic method for improving salvianolic acid B content in root of red-rooted salvia
A salvianolic acid and transgenic technology, applied in genetic engineering, plant genetic improvement, botanical equipment and methods, etc., can solve the problem that a single genetic modification is difficult to work
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[0028] The experimental method that does not indicate specific condition in this embodiment, operates according to known method, such as Sambrook etc. " molecular cloning: laboratory handbook " (New York: Cold Spring Harbor Laboratory Press, 1989) described condition Or operate under the conditions recommended by the manufacturer.
[0029] 1. Cloning of full-length cDNA of Arabidopsis anthocyanin production transcription factor 1 gene
[0030] (1) Extraction of Arabidopsis total RNA
[0031]Select fresh and robust whole plants of Arabidopsis thaliana seedlings, and use the Biozol total RNA extraction kit produced by Hangzhou Biozol Technology Co., Ltd. to isolate and extract their total RNA. The extraction method refers to the Biozol Total RNA Extraction Kit (Cat. # BSC51S1) instructions.
[0032] (a) Preparatory work for extracting total RNA from Arabidopsis thaliana
[0033] All kinds of glass instruments, gun tips, and centrifuge tubes used were washed, soaked in a 0.1...
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