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Use of compound

A compound and drug technology, applied in the field of medical immunology, can solve problems such as body damage, achieve remarkable effects, avoid immune response, and suppress immune response

Inactive Publication Date: 2011-05-25
林治华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the immune response can also be damaging to the body in certain circumstances, such as autoimmune diseases and transplant rejection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: Mouse T cell proliferation inhibition test

[0043] After aseptically separating spleen cells from mice and lysing red blood cells, add them to 96-well culture plates, 1-2×10 5 Cells / 100 μL / well. Add anti-CD3 (final concentration 1 μg / mL), anti-CD28 monoclonal antibody (final concentration 1 μg / mL), recombinant IL-2 (final concentration 10 Unit / mL) to stimulate in vitro, 100 μL / well, suppose to add 2-amino-7-( Hydroxyimino)-4,5,6,7-tetrahydrobenzothiophene-3-ethyl carboxylate was the test group, 3 wells / group; no 2-amino-7-(hydroxyimino)- Ethyl 4,5,6,7-tetrahydrobenzothiophene-3-carboxylate was used as the control group, 3 wells / group. Simultaneously, set as the blank group without the above-mentioned in vitro stimulation and without adding 2-amino-7-(hydroxyimino)-4,5,6,7-tetrahydrobenzothiophene-3-carboxylate ethyl ester, 3 hole / group.

[0044] Three groups of samples were placed at 37°C, 5% CO 2 Incubate for 66h under the condition, add 0.5-1μci to ea...

Embodiment 2

[0046] Embodiment 2: human T cell proliferation inhibition test

[0047] Under sterile conditions, isolate peripheral blood mononuclear cells (PBMC) from heparin anticoagulated blood, and adjust the number of cells to 1-2×10 with 10% FCS RPMI1640 after washing 6 / mE, added to 96-well culture plate, 1-2×10 5 Cells / 100 μL / well. Add T cell mitogen PHA, 100 μL / well, set 2-amino-7-(hydroxyimino)-4,5,6,7-tetrahydrobenzothiophene-3-carboxylic acid ethyl ester as the test group , 3 wells / group; without 2-amino-7-(hydroxyimino)-4,5,6,7-tetrahydrobenzothiophene-3-carboxylate ethyl ester as the control group, 3 wells / group. Simultaneously, set as the blank group without the above-mentioned in vitro stimulation and without adding 2-amino-7-(hydroxyimino)-4,5,6,7-tetrahydrobenzothiophene-3-carboxylate ethyl ester, 3 hole / group.

[0048] Three groups of samples were placed at 37°C, 5% CO 2Incubate for 66h under the condition, add 0.5-1μci to each well 3 H-TdR 50μL, continue to culture...

Embodiment 3

[0050] Example 3: Mouse FoxP3 + CD4 + Regulatory T cell induction assay

[0051] FoxP3 + CD4 + Regulatory T cells are a type of T cells with immunosuppressive effects. This type of cells participates in the regulation of immune response / immune tolerance in an "active" manner, not only participates in the regulation of autoimmune tolerance, but also plays an important role in transplantation immunity. In order to prove whether ethyl 2-amino-7-(hydroxyimino)-4,5,6,7-tetrahydrobenzothiophene-3-carboxylate can induce naive T cells to differentiate into FoxP3 + CD4 + Regulatory T cells, after the present invention isolates mouse splenocytes and stimulates them in vitro, FoxP3 is detected by flow cytometry (FACS) + CD4 + Differentiation of regulatory T cells. Specific steps are as follows:

[0052] According to the method of Example 1, it is divided into a control group, a test group and a blank group, and then cultured in a 24-well plate, 1-2 × 10 6 / mL, 2mL / well. At 37...

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Abstract

The invention relates to the field of medical immunology and discloses the use of a compound of a formula I in the preparation of medicaments for inducing T cells to differentiate into FoxP<3+>CD<4+> regulatory T cells and inhibiting T cell proliferation. The compound of the formula I plays an obvious role in inhibiting T cell proliferation and inducing the T cells to differentiate into FoxP<3+>CD<4+> regulatory T cells, and the medicaments for inducing T cells to differentiate into FoxP<3+>CD<4+> regulatory T cells and inhibiting T cell proliferation, which is prepared from the compound, can be used for treating autoimmune diseases and transplantation rejection.

Description

technical field [0001] The invention relates to the field of medical immunology, in particular to a compound that induces T cells to differentiate into FoxP3 + CD4 + Regulatory T cells and drugs that inhibit T cell proliferation. Background technique [0002] T cells are a type of lymphocytes expressing T cell receptor and CD3 complexes, derived from embryonic liver or bone marrow, differentiate and develop in the thymus microenvironment, migrate to peripheral blood after maturation, and then settle in peripheral lymph Tissues that mediate cellular immune responses and assist the body in generating humoral immune responses against T cell-dependent antigens. [0003] Immune response refers to the whole process in which the immune system of the body receives antigen stimulation, lymphocytes specifically recognize antigens, undergo activation, proliferation, differentiation or incapacity, apoptosis, and then exert biological effects. The most basic biological significance of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/381A61P37/02A61P37/06A61P3/10A61P19/04A61P25/00A61P29/00
Inventor 林治华马正伟许雪青朱波王锐
Owner 林治华
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