Preparation method of Gynura bicolor total flavone extract

A technology of total flavonoids and extracts, which is applied in the field of extraction and preparation of total flavonoids, can solve problems such as the preparation process report of total flavonoids in red phoenix, and achieve the effect of increasing the content of total flavonoids and the content of total flavonoids

Inactive Publication Date: 2011-06-01
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the chemical constituents isolated from the red phoenix mainly include flavonoids and other components, but there is no report on the preparation process of the total flavonoids in the red phoenix

Method used

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  • Preparation method of Gynura bicolor total flavone extract

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preparation example Construction

[0028] Preparation of reference substance solution: Accurately weigh 5.0 mg of rutin standard substance in a 25 mL volumetric flask, ultrasonically dissolve it with 70% ethanol, and constant volume to obtain a concentration of 0.2 mg·mL -1 rutin standard solution.

[0029] Preparation of the sample solution: Precisely pipette 0.5 g of the total flavonoids extract of Pteris vulgaris into a 25 mL volumetric flask, add 70% ethanol to ultrasonically dissolve, and shake well.

[0030] Determination of the total flavonoid content in the sample: Precisely pipette 1ml of the sample solution and add 0.1mol·L -1 AlCl 3 ·6H 2 O 1.0mL, dilute to volume with 70% ethanol, shake well, measure the absorbance at 412nm after standing for 15min, and calculate the total flavonoid content of the sample according to the rutin standard curve.

[0031] The results are shown in Table 1. The total flavonoid content obtained by method D alcohol extraction and water precipitation combined with macrop...

Embodiment 1

[0035] 1.0kg of red phoenix medicinal material coarse powder, soaked in 70% ethanol with a weight ratio of 12 times of the medicinal material for 48 hours, extracted by percolation, until the effluent liquid color is light, and the flavonoid reaction is not obvious, concentrated under reduced pressure to form a thick paste, thicken The weight of the paste is 10 times the weight of water, stirred, and cooled for 12 hours. Put it in a centrifuge for sedimentation and centrifugation (2000 rpm, 15 minutes), and take the supernatant for later use.

[0036] Add the supernatant to 500 g of D101 macroporous resin, elute with 10 times the volume of the macroporous resin, 20% ethanol, 40% ethanol gradient ethanol, and collect the 40% ethanol eluate. Concentrate the 40% ethanol eluate until it has no alcohol smell, add it to 500g D101 macroporous resin, and elute with 20% ethanol and 40% ethanol gradient at 10 times the volume of the macroporous resin respectively, collect the 40% ethano...

Embodiment 2

[0038] 1.0kg of coarse powder of red phoenix herb, heated and refluxed with 70% ethanol with a weight ratio of 12 times of the materials, and extracted twice, each time for 2 hours, the extract was concentrated under reduced pressure to form a thick paste, thickened with 10 times the weight of water, stirred, Allow to cool for 12 hours. Put it in a centrifuge for sedimentation and centrifugation (2000 rpm, 15 minutes), and take the supernatant for later use.

[0039] The supernatant was added to 1.0 kg D101 macroporous resin, and gradient eluted with 20% ethanol and 40% ethanol respectively at 10 times the volume of the macroporous resin, and the 40% ethanol eluate was collected. Concentrate the 40% ethanol eluate until it has no alcohol smell, add it to 500g D101 macroporous resin, and elute with 20% ethanol and 40% ethanol gradient at 10 times the volume of the macroporous resin respectively, collect the 40% ethanol eluate, concentrate and dry to obtain red Fengcai total fl...

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Abstract

The invention discloses a preparation method of a Gynura bicolor total flavone extract. The method comprises the following steps: (1) drying and grinding Gynura bicolor; (2) extracting with ethanol or methanol, mixing extracting solutions, and concentrating under reduced pressure to obtain extractum; (3) adding water in the extractum obtained in the step (2), standing to cool, centrifuging to remove precipitates and obtain supernatant for standby; performing adsorption on the supernatant obtained in the step (3) by utilizing macroporous adsorption resin, and performing gradient elution with ethanol; (5) collecting the ethanolic elution solution obtained in the step (4) to concentrate under reduced pressure, adsorbing with macroporous resin, and performing gradient elution with ethanol; and (6) concentrating and drying the ethanolic elution solution obtained in the step (5) to obtain the Gynura bicolor total flavone extract.

Description

Technical field: [0001] The invention relates to a method for extracting and preparing total flavonoids from traditional Chinese medicine fennel. Background technique: [0002] Red Fengcai (Gynura bicolor DC.) is a perennial herb of Compositae. The whole plant has the effects of promoting blood circulation, stopping bleeding, detoxifying and reducing swelling. The root has the function of promoting qi and activating blood, and is used to treat postpartum abdominal pain, metrorrhagia, and malaria. At present, the chemical constituents isolated from the red phoenix mainly include flavonoids and other components, but there is no report on the preparation process of the total flavonoids in the red phoenix. Invention content: [0003] The present invention provides a kind of preparation method of the total flavonoid extract of red phoenix root, the method comprises the following steps: [0004] (1) dry and pulverize the red phoenix; [0005] (2) Extract with ethanol or metha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61P7/04A61P15/00A61P17/02
Inventor 吕寒李维林陈剑裴咏萍任冰如赵慧芳
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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