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Liriope muscari extract with anti-tumor action and preparation method thereof

A technology for Ophiopogon japonicus, anti-tumor effect, applied in the direction of anti-tumor drugs, medical preparations containing active ingredients, drug combinations, etc., can solve the role that is difficult to teach according to clinical basis, and the tumor cannot prove that the effective parts are equally effective. And other issues

Inactive Publication Date: 2011-06-08
NAT INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] It can be seen that simply using the experimental results of S180 can not be clinically located in any accurate position of cancer / tumor in the human body, and the effect of the monomer on a certain tumor strain does not prove that the effective part is also effective. The present invention will use Detailed data prove this argument, and the medical treatment of cancer / tumor is to be treated strictly according to the growth site or organ of the tumor in the human body. The literature on cancer treatment only plays a leading role in the clinical research of cancer treatment, and it is difficult to consider it as a clinical basis or play a teaching role

Method used

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  • Liriope muscari extract with anti-tumor action and preparation method thereof
  • Liriope muscari extract with anti-tumor action and preparation method thereof
  • Liriope muscari extract with anti-tumor action and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0074] Take the Ophiopogon japonicus medicinal material, add about 15 times the amount of 95% ethanol for reflux extraction; extract the solvent to a thick paste, add the same amount of crude drug (weight) to disperse with water, extract twice with n-butanol, combine the extracts, Recover the solvent to obtain the crude extract. Dissolve the crude extract in water equal to the weight of the crude drug, put it on an AB-8 macroporous resin column, and elute with water until the molish reaction of the eluent is negative, and then use a concentration of 8 times the column volume at 60 % ethanol for elution, and the eluent recovered the solvent to obtain the extract of Ophiopogon japonicus.

[0075] Through adopting the method (HPLC) of high-performance liquid chromatography analysis to detect, chromatographic column C18, mobile phase is the mixed solution of acetonitrile-water, gradient elution, detect through evaporative light detector, obtain collection of illustrative plates se...

Embodiment 2

[0078] Take the Ophiopogon japonicus medicinal material, add about 8 times the amount of 75% ethanol to immerse and extract twice; extract the solvent to a thick paste, add 0.5 times the crude drug amount (weight) to disperse with water, extract 3 times with n-butanol, and combine extract, and recover the solvent to obtain the crude extract of Liepogon japonicus. Dissolve the crude extract in 0.5 times the weight of the crude drug in water, put it on a D-101 macroporous resin column, and elute with water until the molish reaction of the eluent is negative, and then use a volume concentration of 10 times the column volume to dissolve the crude extract in water. Elute with 80% ethanol, and recover the solvent from the eluent to obtain the extract of Ophiopogon japonicus.

[0079] Through adopting the method (HPLC) of high-performance liquid chromatography analysis to detect, chromatographic column is C18, and mobile phase is the solution of methanol-water gradient elution, and u...

Embodiment 3

[0081] Take Duantingshan Ophiopogon japonicus medicinal material, add about 4 times the amount of 80% ethanol to soak overnight, then heat and extract; extract the solvent to a thick paste, add 1.5 times the crude drug amount (weight) to disperse with water, extract 4 times with n-butanol, The extracts were combined, and the solvent was recovered to obtain the crude extract of Liepogon japonicus. Dissolve the crude extract in water with 1.5 times the weight of the crude drug, put it on a D-101 macroporous resin column, and first elute with water until the molish reaction of the eluent is negative, then use 50% of 15 times the column volume Concentration of ethanol elution, the eluent recovery solvent, to get short Tingshan Ophiopogon japonicus extract.

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Abstract

The invention provides a liriope muscari extract with anti-tumor action, which is prepared by a method with the following steps: taking liriope muscari roots as raw materials, extracting the raw materials with ethanol, and extracting the extracting solution with n-butyl alcohol to obtain a crude extract; adding the crude extract into water for dissolving; enabling the crude extract solution to pass through a non-polar macroporous resin column; firstly, eluting with water until the eluent is colorless, and then, eluting with the water solution of the ethanol; and collecting the eluent until the eluent is colorless, thereby obtaining the liriope muscari extract. The invention also provides a preparation method of the liriope muscari extract.

Description

technical field [0001] The present invention provides an extract of Ophiopogon japonicus with anti-tumor effect and its preparation method, especially the extract of Ophiopogon japonicus Duantuishan containing steroidal saponins and sesquiterpene glucosides, and the extract method of preparation and purification. Background technique [0002] Liriope musacarli (Decne.) Bailey belongs to Liriope musacarli (Decne.) Bailey, and its roots have always been used as a substitute for Liriope musacarli. Dong is one of the sources of medicinal materials of Liedong. [0003] Ophiopogon japonicus saponin C is a monomeric compound extracted from Ophiopogon japonicus. Studies have shown that it has good anti-S180 solid tumor and Ehrlich ascites carcinoma activity. [0004] The extraction method of Ophiopogon japonicus saponin C is to extract the coarse powder of Ophiopogon japonicus luteus with methanol first, then extract with ether and n-butanol in turn, and the n-butanol extract is s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/896A61K31/7048A61P35/00
Inventor 王钢力林瑞超聂黎行谢文利杜树山丁锐
Owner NAT INST FOR FOOD & DRUG CONTROL
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