Silk ligament and preparation method thereof

Abstract

The invention provides a silk ligament and a preparation method thereof, and relates to the field of prosthetic materials. The silk ligament is prepared from natural silk serving as raw materials by stranding, twisting, degumming and bone morphogenetic protein (BMP) filling, and consists of lead wires (1), an inner core (2) and a core shell (3), wherein the lead wires (1), the inner core (2) and the core shell (3) are formed by weaving integrally; the core shell (3) is fixed on the middle part of the inner core (2); and the lead wires (1) are fixed at both ends of the inner core (2). Compared with the conventional artificial ligament, the mechanical property of the silk ligament are in stricter accordance with the properties of anterior cruciate ligaments (ACL) of human bodies, and after being filled with the BMP, the silk ligament rebuilding is more convenient.

Description

technical field [0001] The invention relates to the field of prosthesis materials, in particular to a silk ligament and a preparation method thereof. Background technique [0002] The cruciate ligament is located in the knee joint and has a stabilizing effect on the connection of the knee joint. In daily life, the probability of ligament injury is quite high, which directly leads to joint instability and joint inflammation. However, the self-repair ability of the ligament is very poor. The ligament needs to be replaced by surgery to restore its function. Due to the problems of insufficient source, high risk, and immune rejection of the homograft, its clinical application is relatively limited. Therefore, artificial ligaments made of fibers and textile materials are used. To replace the injured ligament has become the mainstream ligament repair treatment. Due to the good biocompatibility and cell affinity of silk fibroin fibers, choosing silk as the matrix of artificial liga...

AI Technical Summary

Problems solved by technology

[0002] The cruciate ligament is located in the knee joint and has a stabilizing effect on the connection of the knee joint. In daily life, the probability of ligament injury is quite high, which directly leads to joint instability and joint inflammation. However, the self-repair ability of the ligament is very poor. The ligament needs to be replaced by surgery to restore its function. Due to the problems of insufficient source, high risk, and immune rejection of the homograft, its clinical application is relatively limited. Therefore, artificial ligaments made of fibers and textile materials are used. To replace the damaged ligament and become the mainstream ligament repair treatment

Due to the good biocompatibility and cell affinity of silk fibroin fibers, choosing silk as the matrix of artificial ligaments has become the main direction of the existing artificial ligament research. In 2007, the silk tendon developed by Tianjin Medical University in China has intermediate limitations due to structural limitations. To solve the problem that the dense part of the cells cannot grow into, Singapore Hongbing Fan et al. used bone marrow mesenchymal stem cells and silk scaffolds to reconstruct animal ACL (anterior cruciate ligament ACL for short) experiment, the silk was initially twisted into cloth, and then along a central axis Rolled into a column, the defect of this silk ligament is that it cannot meet the mechanical requirements of clinical ACL reconstruction

Although the silk ligament has the characteristics of cell affinity and biocompatibility, the seed cells can attach and proliferate well on the surface of the ligament scaffold, but its internal dense structure makes it very difficult for the cells to grow inward, which is not conducive to the formation of a three-dimensional cell network. Moreover, During the operation, the implantation of the ligament requires additional guide wire traction. Adding a guide wire during the operation is not only a waste of time, but also easily causes infection. Therefore, there is still a lot of room for improvement in the design of the silk ligament

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