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Method for enriching and purifying forskolin in Rosa davurica flower

A technology of rose fruit flower and enrichment, applied in organic chemistry and other fields, can solve the problems of low extraction rate and low extraction purity, and achieve the effects of high extraction purity, good thermal stability, fast adsorption and fast resolution

Active Publication Date: 2011-07-20
DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the shortcomings of conventional techniques such as relatively low extraction rate and low extraction purity, and provide a method for enriching and purifying forskolin in Rosa rosea by using macroporous adsorption resin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: a kind of method that utilizes macroporous adsorption resin to enrich and purify forskolin in rose fruit flower, its steps are as follows:

[0027] (1) After crushing the dried Rosa rosea flower, pass it through a 60-mesh sieve, heat and reflux extract twice with acid water with pH=4 at the ratio of material:water=1:12, each time for 2 hours, and combine the extracts;

[0028] (2) Filtrating the Rosa rosea flower extract treated in (1), drying under reduced pressure to obtain the initial extract in the form of an extract;

[0029] (3) Dissolving the harvested primary extract in 75% ethanol for decolorization, concentrating the decolorization solution to recover ethanol, and obtaining the crude extract of forskolin.

[0030] (4) The obtained forskolin extract is adsorbed on a macroporous adsorption resin chromatography column, and the macroporous resin used is the NKA-II type resin produced by Tianjin Nankai University;

[0031] (5) Carry out gradient elut...

Embodiment example 2

[0035] Implementation case 2: a method for enriching and purifying forskolin in Rosa rosea by using macroporous adsorption resin, the steps are as follows:

[0036] (1) Pass through a 10-mesh sieve after crushing the dried Rosa rosea fruit flower, heat and reflux the extract with PH=4 acid water at a ratio of material:water=1:12 for 2 times, each time for 2 hours, and combine the extracts;

[0037] (2) Filtrating the Rosa rosea flower extract treated in (1), drying under reduced pressure to obtain the initial extract in the form of an extract;

[0038] (3) Dissolving the harvested primary extract in 75% ethanol for decolorization, concentrating the decolorization solution to recover ethanol, and obtaining the crude extract of forskolin.

[0039] (4) The obtained forskolin crude extract is adsorbed with a macroporous adsorption resin, and the macroporous resin used is the D4006 type resin produced by Tianjin Nankai University;

[0040] (5) Carry out eluting with 10%, 30%, 50%,...

Embodiment example 3

[0044] Implementation case 3: a method for enriching and purifying forskolin in Rosa rosea by using macroporous adsorption resin, the steps are as follows:

[0045] (1) After crushing the dried Rosa rosea flower, pass it through an 80-mesh sieve, heat and reflux the extract with pH=4 at the ratio of material:water=1:12 for 2 times, each time for 2 hours, and combine the extracts;

[0046] (2) Filtrating the Rosa rosea flower extract treated in (1), drying under reduced pressure to obtain the initial extract in the form of an extract;

[0047] (3) Dissolving the harvested primary extract in 75% ethanol for decolorization, concentrating the decolorization solution to recover ethanol, and obtaining the crude extract of forskolin.

[0048] (4) the obtained forskolin crude extract is adsorbed on a macroporous adsorption resin chromatography column, and the macroporous resin used is the NKA-9 type resin produced by Tianjin Nankai University;

[0049] (5) Use 20%, 40%, 60%, and 80% ...

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PUM

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Abstract

The invention belongs to the oranic chemistry field of natural products and relates to a method for enriching and purifying forskolin in Rosa davurica flower by utilizing macroporous adsorption resin. The method is characterized in that the macroporous adsorption technology is used to separate and purify forskolin, thus the adsorption selectivity on forskolin is good; adsorption and desorption are performed fast and the adsorption capacity is larger; and forskolin is convenient and fast to be extract, the raw material sources are rich, the production cost is low, the separation effect is obvious, the extraction purity is high, and the content of a forskolin is more than 35% in forskolin semi-finished product and a forskolin of which content is more than 90% in forskolin finished product can be obtained, and the defects of the conventional extraction method, namely lower extraction efficiency and low extraction purity can be overcome. The method selects the macroporous adsorption resinwith stable physical and chemical properties, larger surface area, higher exchange speed, high mechanical strength, high contamination resistance and good thermal stability to selectively adsorb forskolin from solution through physical absorption, thus absorption and desorption can be performed fast and the adsorption capacity is larger.

Description

Technical field: [0001] The invention belongs to the field of natural organic chemistry, and relates to a method for purifying forskolin, in particular to a method for enriching and purifying forskolin in Rosa rosea by using a macroporous adsorption resin. Background technique: [0002] Natural thorn fruit, scientific name wild rose, also known as wild thorn fruit, mountain thorn rose fruit, is a member of the family Rosaceae. Rose thorn flower is the flower of Rose thorn, which can extract the essential oil of Rose thorn, which sells well at home and abroad, and the price is higher than gold; it can also extract pigments and essences for food and beverages. The main chemical components are forskolin, iso Forskolin, Forskolin A, Forskolin B and other ingredients. [0003] Forskolin is the strongest adenylate cyclase activator known so far, it can directly stimulate adenylate cyclase, increase the concentration of cyclic adenylate in various tissue cells, and thus participat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/94
Inventor 姚德坤姚德利闫峰黄运生刘德文姚航郑君
Owner DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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