Method for improving soybean quality and DNA (Deoxyribonucleic Acid) molecule special for same

A technology of DNA molecules and DNA sequences, which is applied in the field of plant genetic engineering and can solve problems such as the production of trans fatty acids

Inactive Publication Date: 2011-07-20
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although hydrogenation can reduce the number of double bonds, the hydrogenation process produces trans fatty acids

Method used

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  • Method for improving soybean quality and DNA (Deoxyribonucleic Acid) molecule special for same
  • Method for improving soybean quality and DNA (Deoxyribonucleic Acid) molecule special for same
  • Method for improving soybean quality and DNA (Deoxyribonucleic Acid) molecule special for same

Examples

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Embodiment Construction

[0028] The experimental methods used in the following examples are conventional methods unless otherwise specified.

[0029] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0030] Restriction enzymes EcoRV, BamHI, ClaI, KpnI, XhoI, enzymes used for ligation and filling, and TA VectorI vector were all purchased from TaKaRa Company.

[0031] The plant expression vector pKANNIBAL is described in Wesley SV, Helliwell CA, Smith NA, Wang MB, Rouse DT, Liu Q, Gooding PS, Singh SP, Abbott D, Stoutjesdijk PA, Robinson SP, Gleave AP, Green AG, Waterhouse PM (2001) Construct design for efficient, effective and high-throughput gene silencing in plants. Plant J 27(6):581-590, publicly available from Institute of Botany, Chinese Academy of Sciences.

[0032] Plant expression vector pBI121-Lec is recorded in Liu Q, Singh SP, Green AG (2002) High-stearic and high-oleic cottonseed oils produced by hairpin RNA-medi...

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Abstract

The invention discloses a method for improving soybean quality and a DNA (Deoxyribonucleic Acid) molecule special for the same, and provides a DNA molecule which is: (1) a DNA molecule shown as a sequence I in a sequence table or (2) a DNA molecule which is hybridized with the DNA sequence defined in (1) under the strict condition and has the same function as the DNA sequence defined in (1) or (3) a DNA molecule having at least 70 percent, at least 75 percent, at least 80 percent, at least 85 percent, at least 90 percent, at least 95 percent, at least 96 percent, at least 97 percent, at least 98 percent or at least 99 percent of homology and the same function as the DNA sequence defined in (1). An experiment in the invention indicates that a genetically engineered soybean seed of a transgenic soybean delta12-desaturase gene (FAD2-1) silent expression vector shows a high oleic acid phenotype, and transgenic soybean seed oil remarkably differs from wild type soybean in the aspects of oleic acid, linoleic acid, linolenic acid and palmitic acid.

Description

technical field [0001] The invention relates to the field of plant genetic engineering, in particular to a method for improving soybean quality and a special DNA molecule thereof. Background technique [0002] Soybean [Glycine max (L.) Merr.] is an annual leguminous plant, which has been planted in China for more than 4,700 years. Soybean is an important oil crop and food crop, and an important source of edible oil and vegetable protein in the world. With the development of the economy and the improvement of living standards, people not only greatly increase the demand for soybeans, but also put forward higher requirements for the quality of soybeans. The quality of soybean mainly depends on the protein, fat content and fatty acid composition in its grain. [0003] There are a variety of fatty acids in nature, and the daily edible vegetable oil is mainly composed of 5 kinds of fatty acids, accounting for more than 90% of the total fatty acids. They are: palmitic acid (C16:...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12N15/63C12N15/82
Inventor 许亦农吴韩英王戈亮刘超武旺赵明明李敏春
Owner INST OF BOTANY CHINESE ACAD OF SCI
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