Method for detecting water inherent toxicity by using single cell gel electrophoresis test
A single-cell gel and genetic toxicity technology, which is applied in the field of water toxicity detection, can solve the problems of difficult unification and insufficient intuitive toxicity, and achieve the effects of easy unification of test results, quantification, and accurate water toxicity
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Embodiment 1
[0037] Take daily urban drinking tap water as the water sample to be tested, and carry out concentration and enrichment treatment on the water sample. The process is as follows: take 100L water sample, and use the activated XAD-2 resin for adsorption at a rate of 40mL / min. After adsorption, use acetic acid Ethyl ester was eluted, and the eluate was concentrated to 10 mL at 50°C.
[0038] This specific embodiment adopts the following steps:
[0039] (1) Select the lymphocytes of the spleen of mice as the indicator organism, and take 6 concentration points within the range of chromium ion concentration 0.025-0.5mg / L to treat the mice for 5 days respectively. The ion concentrations are 0.025mg / L, 0.05mg / L, 0.1mg / L, 0.15mg / L, 0.25mg / L, 0.5mg / L;
[0040] (2) Preparation of single cell suspension: Cut out the spleen of the mouse, rinse with phosphate buffer, add 1mL of phosphate buffer, cut it into pieces with ophthalmic scissors, absorb the supernatant, add lymphocyte extract to t...
Embodiment 2
[0050] Take the influent water from the daily urban water plant as the water sample to be tested, and carry out concentration and enrichment treatment on the water sample. The process is as follows: take 40L water sample, and use the activated XAD-2 resin for adsorption at a rate of 40mL / min. After that, it was eluted with ethyl acetate, and the eluate was concentrated to 10 mL at 50°C.
[0051] Adopt and embodiment 1 method to draw Cr 6+ Standard curve for olive tail distance, the curve with figure 1 Basically the same, and then the concentrated and enriched water samples were exposed to the mice, the steps were the same as in Example 1, the olive tail distance of the obtained water samples to be tested was 50.1, and the experimental results were substituted into Cr 6+ To the standard curve of olive tail distance, the water quality toxicity of this water sample is equivalent to 0.225mg / LCr 6+ toxicity.
Embodiment 3
[0053] Take the effluent of the productive test process of the daily urban water plant as the water sample to be tested, and carry out concentration and enrichment treatment on the water sample. The process is as follows: take 20L water sample, and use the activated XAD-2 resin for adsorption at a rate of 40mL / min , after adsorption, it was eluted with ethyl acetate, and the eluate was concentrated to 10 mL at 50 °C.
[0054] Adopt and embodiment 1 method to draw Cr 6+ Standard curve for olive tail distance, the curve with figure 1 Basically the same, and then the concentrated and enriched water sample was poisoned to the mice, the steps were the same as in Example 1, the olive tail distance of the obtained water sample to be tested was 40.1, and the experimental results were substituted into Cr 6+ To the standard curve of olive tail distance, the water quality toxicity of this water sample is equivalent to 0.102mg / LCr 6+ toxicity.
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