Method for detecting electromagnetic radiation bioluminescence effect in real time

A technology of electromagnetic radiation and bioluminescence, applied in the field of biomedical detection technology, can solve problems such as difficulty in obtaining transient or instant change information, loss of luminescence change information, etc.

Inactive Publication Date: 2011-09-07
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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Problems solved by technology

However, this bioluminescence process usually changes quickly. When the external stimulus is removed, the luminescence disappears immediately. At present, when using a luminescence measuring instrument to measure the relationship between electromagnetic radiation and bioluminescence, the sample is first subjected to electromagnetic radiation, and then moved into the Measurement of luminescence instrument Dark room measurement, the sample is subjected to radiation treatment and luminescence detection is time-sharing, it is difficult to obtain the transient or instant change information of bioluminescence when the organism receives electromagnetic radiation, resulting in the loss of some biologically important luminescence change information

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  • Method for detecting electromagnetic radiation bioluminescence effect in real time
  • Method for detecting electromagnetic radiation bioluminescence effect in real time

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Embodiment Construction

[0009] Combine below figure 1 Further illustrate the preferred solution for realizing the present invention.

[0010] Remove the top cover of the luminescence darkroom of the luminometer, drill a hole on the cover facing the sample cell and install a microwave coaxial cable socket (2) to connect it to the microwave power amplifier, and fix a square microstrip antenna on the inner side of the cover (3), the size is 40×40mm. The sample cell (4) adopts a quartz glass sample cell with a diameter of 40 mm, and the height of the gasket (5) is 50 mm, so that the distance between the sample and the antenna 6 pieces / ml. The electromagnetic radiation is S-band microwave, the frequency is 2.85GHz, the radiation mode is pulse modulation, the pulse width is 2μS, and the peak output power is 100W. When operating, close the darkroom after placing the sample, do not turn on the electromagnetic radiation, record the luminescence background value of the sample, keep the luminometer working in...

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Abstract

The invention relates to a method for detecting an electromagnetic radiation bioluminescence effect in real time, which comprises the following steps of: arranging a microwave antenna in a dark chamber of a luminescence instrument to make electromagnetic radiation energy directly and closely acted on a biological sample in the dark chamber, and detecting change information of bioluminescence in real time when electromagnetic radiation acts on the biological sample. The irradiation distance is controlled by an organic glass gasket, and an electromagnetic wave absorbing material is filled in the bin of the dark chamber to prevent the scattering effect of electromagnetic waves. The measurable biological sample comprises a biological tissue sample, a cell and subcell sample, a biological macromolecular sample and a small-volume animal sample. By the method, a new technical method and a new analysis index can be provided for researching an electromagnetic radiation biological effect mechanism.

Description

technical field [0001] The invention relates to a real-time detection method for the biological effect of electromagnetic radiation, which studies the mechanism of the biological effect by measuring the instantaneous bioluminescent change caused by electromagnetic radiation in real time, and belongs to the technical field of biomedical detection technology. Background technique [0002] Electromagnetic radiation can produce a variety of biological effects on organisms, and the research on the mechanism of its biological effects has become a focus of attention in recent years. [0003] Bioluminescence is a special biological phenomenon in the process of the organism's own metabolism and energy exchange with the outside world. When organisms receive electromagnetic radiation, the absorption, transmission and transformation of electromagnetic energy may cause changes in bioluminescence. Therefore, the instantaneous bioluminescence changes caused by electromagnetic radiation can...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/42C12M1/34C12Q1/02
CPCC12M35/02
Inventor 吴可张清俊丛建波王长振先宏
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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