Expression and application of human papilloma viruses type 16 and 18 L1 proteins in inset cells

A technology of L1 protein and insect cells, applied in the fields of application, antiviral agents, viral antigen components, etc., can solve the problems of high cost and difficult to clearly prove the protective effect, and achieve the effect of increasing the expression level
CN102178944AInactive Publication Date: 2011-09-14DALIAN ALEPH BIOMEDICAL
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
DALIAN ALEPH BIOMEDICAL
Publication Date
2011-09-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for preparing a bivalent vaccine for human papilloma viruses HPV16 and HPV18, which comprises the following steps of: optimizing gene sequences of L1 proteins of the HPV16 and HPV18; cloning the optimized gene sequences into a baculovirus expression vector and transfecting the inset cells to obtain a recombinant baculovirus; infecting the insect cells by using the baculovirus; and recovering and purifying the L1 proteins expressing the HPV16 and HPV18, and mixing with an adjuvant to prepare the bivalent vaccine. The invention also provides the optimized gene sequences of the L1 proteins of the HPV16 and HPV18, vectors containing the gene sequences, and cells containing the vectors.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and relates to the expression of human papillomavirus type 16 and type 18 L1 proteins in insect cells and a vaccine preparation method thereof. Background technique

[0002] Statistics from the World Health Organization (WHO) show that cervical cancer is an invasive disease that seriously endangers women's health. Its incidence rate is second only to breast cancer, ranking second among female malignant tumors in the world, and even in some developing countries. first place. Cervical cancer is one of the most common gynecological malignancies. About 500,000 women around the world suffer from cervical cancer and nearly 300,000 women die from cervical cancer every year. Among them, 80% of the deaths occur in developing countries. According to incomplete statistics, there are about 138,000 cervical cancer patients in my country, and about 50,000 people die of cervical cancer every year, and the incidenc...

Claims

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