Complex enzyme additive for tobacco and preparation method and application thereof
A technology of compound enzymes and additives, applied in the fields of biochemical equipment and methods, enzymes, enzymes, etc., can solve the problems that have not yet been discovered, and achieve the effects of strong standardization and controllability, promotion of transformation, and increase of content.
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Embodiment 1
[0021] ——Preparation of compound enzyme additive
[0022] 1. Please refer to Table 1 for the information of the biological enzyme preparations to be selected.
[0023]
[0024] Screening of biological enzyme preparations
[0025] ① According to the weight ratio, dilute each biological enzyme preparation with deionized water to a ratio of 1:500, 1:2000, 1:4000, 1:6000), and adjust the pH value with citric acid to adjust to the optimum reaction pH value.
[0026] ② Weigh 50 g of tobacco leaf sample, and measure the moisture content of the sample.
[0027] ③The prepared biological enzyme preparation was evenly sprayed on the surface of 50 g cut tobacco, and the final moisture content was controlled to be 20% as the treatment sample; the same amount of deionized water was directly sprayed as the blank control sample. The samples were packed into sealed bags respectively, and placed in an oven at 50° C. for 2 hours at a constant temperature.
[0028] ④ Take out the sample an...
Embodiment 2
[0037] ——The role of compound enzyme additive in the alcoholization of tobacco leaves
[0038] Using the assembled biological enzyme preparation as tobacco alcoholizing agent, carry out alcoholization experiment for two months, the specific experimental steps are as follows:
[0039] 1) Weigh 200 g of tobacco leaves, measure the moisture content of each tobacco leaf sample with a rapid moisture analyzer, and calculate the amount of enzyme solution based on the moisture content.
[0040] 2) Prepare a compound enzyme combined enzyme solution (FHM), measure the appropriate enzyme solution and evenly spray it on the surface of 200 g cut tobacco (control the moisture content of cut tobacco at 20%), and mix it evenly as a treatment sample.
[0041] 3) Spray the same amount of deionized water directly (to control the moisture content of cut tobacco to 20%) as a control blank.
[0042] 4) Seal the samples in sealed bags and keep the temperature at 50°C for 2 hours.
[0043] 5) Take ...
Embodiment 3
[0053]The compound enzyme additive FHM-1 prepared in Example 1 was used to treat Shiyi K326 B4F tobacco leaves. The treatment method was the same as above. After 8 months of treatment, the conventional chemical components of the control sample, the treatment sample and the tobacco leaf samples of natural aging for 18-24 months were respectively detected. , combined with sensory evaluation to evaluate the effect of compound enzyme additive FHM-1. The detection results of conventional chemical components showed that after Shiyi K326 B4F tobacco leaves were treated with compound enzyme additive FHM-1 for 8 months, the content of biomacromolecular compounds in tobacco leaves changed greatly, among which the content of reducing sugar and total sugar The content of total nitrogen, cellulose and nicotine has decreased, and the total amount of volatile alkali and petroleum ether extract has decreased. The changes in the content of these compounds are conducive to the smoking quality of...
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