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Method for simultaneously dyeing mycelial masses and starch in mycorhiza of arethusa

A technology of mycelium and mycorrhizal, which is applied in biochemical equipment and methods, determination/inspection of microorganisms, etc., can solve the problem of not being able to observe mycelium and starch at the same time.

Inactive Publication Date: 2013-04-10
GUIZHOU FRUIT INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the defect that the prior art cannot observe the mycelial mass and starch in the mycorrhiza at the same time, and provide a method that can simultaneously dye the mycelial mass and starch

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  • Method for simultaneously dyeing mycelial masses and starch in mycorhiza of arethusa
  • Method for simultaneously dyeing mycelial masses and starch in mycorhiza of arethusa

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Embodiment Construction

[0019] The technical solutions of the present invention will be described in detail below in conjunction with specific embodiments.

[0020] First prepare the dye, the specific preparation method is as follows:

[0021] Chloral hydrate iodine liquid dye: Accurately weigh 1.5g iodine and 5g potassium iodide, put them into a clean beaker for grinding, add 100ml deionized water to dissolve, then add 100g chloral hydrate to mix, dissolve, and store in brown Reserve in bottle.

[0022] Aniline blue glycerin lactic acid dye: accurately weigh 0.04g of water-soluble aniline blue, put it into a clean beaker, add 10ml of deionized water, stir to dissolve, then add 10ml of lactic acid, 20ml of glycerin, mix, that is, put in drops Reserve in bottle.

[0023] Dyeing method:

[0024] 1. Take the healthy milky white roots of orchid plants, and wash the dust outside the roots under tap water;

[0025] 2. Use the freehand section method to slice, select thin and complete slices and place t...

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Abstract

The invention discloses a method for simultaneously dyeing mycelial masses and starch in mycorhiza of arethusa, which comprises the following steps: taking a mycorhiza slice, placing on a glass slide, dripping chloral hydrate iodine solution dye, dyeing, then sucking away the redundant dye, further dripping aniline blue lactoglycerin solution dye, dyeing, then sucking away the redundant dye, and placing under an optical microscope for micro-observation, so that the starch in the mycorhiza is in dark brown and the fungal mycelial masses are in blue. By adopting the method, the mycelial masses and the starch in the mycorhiza can be simultaneously dyed, and the colonization characteristics of fungi of the mycorhiza in a root of the arethusa and the starch distribution characteristics can be simultaneously observed, thereby having important significance for revealing the relationship between digestion and formation of the fungi of the mycorhiza and accumulation and the digestion of a hostcarbohydrate.

Description

technical field [0001] The invention relates to the technical field of mycorrhizal dyeing, in particular to a method for simultaneous dyeing of orchid mycorrhizal mycelium and starch. Background technique [0002] Orchids and fungi have long-term symbiosis to form mycorrhizal structures, and mycorrhizal fungi exist in the root cortex cells in the form of mycelium. Mycorrhizal fungi and their hosts are mutually beneficial, exchanging carbohydrates. Therefore, studying the relationship between mycorrhizal fungi and host carbohydrates from the perspective of cytology is very important for understanding the symbiotic relationship between fungi and plants. Generally, iodine solution is used to stain starch into blue or purple red, and toluidine blue, acid fuchsin, etc. are used to stain fungal tissues. In terms of plant mycorrhizae, acid fuchsin milk phenol oil, aniline blue, etc. are generally used for staining mycorrhizal fungi. [0003] At a certain stage of mycorrhizal gro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/02
Inventor 桂阳朱国胜刘作易
Owner GUIZHOU FRUIT INST
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