Preparation method of free flow electrophoresis chip with two-dimensional depth
An electrophoretic chip and chip technology, which is applied in the preparation of test samples, material analysis by electromagnetic means, and measurement devices, can solve the problems of complicated operation and low success rate, and achieve the effect of simplifying the manufacturing process and reducing the cost of experiments
Inactive Publication Date: 2013-09-18
BEIJING INSTITUTE OF TECHNOLOGYGY
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[0006] The purpose of the present invention is to provide a method for preparing a two-dimensional deep free-flow electrophoresis chip, which does not require the use of complex instruments and equipment and strict The free-flow electrophoresis chip with two deep structures can be fabricated quickly and easily
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[0016] Below in conjunction with accompanying drawing, the invention is described in further detail.
[0017] 1. Mask design: The production of the present invention involves two photolithography, and two masks need to be designed according to the difference in the transferred structure. The mask graphics were drawn with the drawing software Adobe Illustrator CS, and a film-type photolithography mask was made by a high-resolution laser printer. Mask 1 contains structures such as electrode holes, buffer inlet holes, electrode buffer channels, and fraction collection ports (fraction collection ports can be selectively added according to chip function requirements) (the structure diagram is shown in figure 1 As shown), select the corresponding transparent area and opaque area in the graphics area according to the properties of the photoresist sacrificial layer. The graphic area of mask 2 is a black opaque area, which is used to block functional areas such as the buffer inlet, ...
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The invention belongs to the technical field of processing and manufacturing of microfluidic chips, and in particular relates to a preparation method of a free flow electrophoresis chip with two-dimensional depth. The method comprises the following steps of: transferring a channel structure pattern onto a glass plate by adopting a standard photoetching-wet etching method to form a microchannel, and punching holes to manufacture a chip top plate; then fixing a platinum electrode slice between an upper plate and a lower plate of the chip, or combining the chip roof plated with the electrode and a substrate, and filling ultraviolet curing glue between a gap between the upper plate and the lower plate through siphon action, wherein a glass polished slice is used as the chip substrate; protecting a functional region of the free flow electrophoresis chip with a mask film and sealing-in the chip by ultraviolet irradiation; and finally flushing optical glue, which is not polymerized and condensed, out of a cavity of the chip by utilizing an organic solvent, standing and ageing for later use. By means of the preparation method disclosed by the invention, the free flow electrophoresis chip with two depth structures can be rapidly, simply and conveniently prepared without complex instruments and equipment and strict experiment conditions.
Description
technical field [0001] The invention belongs to the technical field of processing and manufacturing microfluidic chips, and relates to a low-cost and rapid preparation method for a two-dimensional deep free flow electrophoresis chip. Background technique [0002] Free-flow electrophoresis (Free-Flow Electrophoresis, FFE) is a continuous separation preparation technology. Its basic principle is: a thin rectangular cavity is formed by two upper and lower parallel plates as a separation chamber, and the compound to be analyzed is separated from the cavity along with the carrier electrolyte. One end of the chamber flows in, and a separation voltage is applied in the vertical direction of the fluid. The sample is deflected in its flow direction according to its charged state, and the separated compound is collected at the outlet end. [0003] Since the technology was proposed in the 1950s and 1960s, it has been widely used in the field of analysis, including cells, peptides, enzy...
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IPC IPC(8): G01N1/28G01N27/447
Inventor 邓玉林丁惠徐建栋吕雪飞胡晓明耿利娜李勤
Owner BEIJING INSTITUTE OF TECHNOLOGYGY