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Method for detecting antibiotic residue in honey based on rapid classification extraction technology

A technology of technical detection and rapid classification, applied in the direction of measuring devices, material separation, and analysis of materials, can solve problems affecting the quality of genuine honey, improve the level of pollutant detection technology and monitoring efficiency, shorten extraction time, and reduce extraction work volume effect

Inactive Publication Date: 2013-11-20
JIANGNAN UNIV +1
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  • Claims
  • Application Information

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Problems solved by technology

[0012] It should be pointed out that most beekeepers can adhere to scientific medication in the production process of bees and treat a small number of honey produced after medication separately. Therefore, the honey containing antibiotics is only It accounts for a very small number. From the quantitative point of view, the proportion of this small amount of honey containing antibiotic pollutants is very small, but if it is mixed into a large amount of genuine honey, it will seriously affect the quality of genuine honey, and the consequences will be very serious.

Method used

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  • Method for detecting antibiotic residue in honey based on rapid classification extraction technology
  • Method for detecting antibiotic residue in honey based on rapid classification extraction technology
  • Method for detecting antibiotic residue in honey based on rapid classification extraction technology

Examples

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Embodiment 1

[0035] Example 1 The standard spectrum of a certain blank honey

[0036] ①Classified extraction of antibiotic residues in honey:

[0037] Extract the blank honey sample of a certain honey with organic solvent ethyl acetate or dichloromethane under the conditions of pH7.0, pH2.5, and pH9.0 respectively, and extract most of the antibiotic residues in the blank honey sample into the solvent , to obtain a sorted extract containing antibiotic residues, which is reserved for subsequent analysis and detection;

[0038] ②Rapid primary screening of antibiotic residues:

[0039] The classified extract containing antibiotic residues obtained in step ① is detected by high performance liquid chromatography separation and detection technology, high performance liquid chromatography-mass spectrometry technology, portable gas chromatography or gas chromatography-mass spectrometry technology to obtain the honey blank The standard map of honey, such as image 3 shown.

Embodiment 2

[0040] Example 2 Standard Spectrum Library of Antibiotic Residues

[0041] According to the same classification and extraction technology as in Example 1 and the rapid detection technology of antibiotic residues, the honey sample after accurately adding a certain antibiotic in the blank honey sample of a certain honey used in Example 1 is extracted and detected for antibiotic residues, and the antibiotic residues are obtained. Standard spectral library of honey with antibiotics.

[0042] Carry out the extraction and detection of antibiotic residues to the blank honey after adding 23 kinds of antibiotics one by one, obtain the standard spectrum that contains various antibiotic residues; 23 kinds of antibiotic standard mixture residue chromatograms, such as Figure 4 shown.

[0043] The standard curve data of 23 antibiotics are shown in Table 1.

[0044] Described 23 kinds of antibiotics are: 1, metronidazole; 2, sulfadiazine; 3, sulfapyridine; 4, oxytetracycline; 5, enoxacin;...

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Abstract

The invention discloses a method for detecting antibiotic residue in honey based on a rapid classification extraction technology, which belongs to the technical field of honey quality detection. The method comprises the following steps of: firstly establishing a standard atlas library of blank honey and a standard atlas library of antibiotic residue after accurately adding one antibiotic, then comparing a detecting image of the to-be-detected honey sample with the standard atlas library of the antibiotic residue to judge whether the antibiotic content in the to-be-detected honey sample exceeds the standard. Partial sample containing pollutant can be rapidly identified in low cost by using the method, the extraction work load is greatly reduced, the extraction time of the pollutant is shortened, the detection efficiency is improved and the detection cost is reduced; a rapid pollutant detection monitoring technology system in the honey in high throughput and low cost for honey export enterprises is established, the defects that the pollutant detection efficiency is low, the detection target is single, the price is expensive and the detection time is long are changed; therefore, the method can provide technical support for the safety production of the exported honey.

Description

technical field [0001] The invention discloses a method for detecting antibiotic residues in honey based on rapid classification and extraction technology, which belongs to the technical field of honey quality detection. Background technique [0002] Honey is a viscous, sweet liquid that is fully brewed by bees by collecting plant pollen and the secretion of living plants, and is fully brewed by the special substances contained in it. It is a natural sweet food that is beneficial to human health. As a sweet component and functional component, it is widely used in food, medicine and health care products industries. [0003] my country's annual output of honey is about 300,000 tons. There are about 300,000 beekeepers and more than 2,000 bee product processing enterprises in the country, with an annual output value of about 8 billion yuan. At present, in the world market, the annual trade volume of honey is about 230,000 tons, and my country’s annual export is about more than ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/02
Inventor 童群义陈金林卢坤陈大伟
Owner JIANGNAN UNIV
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