Application of harpagoside in pharmacy
A technology of harpagoside and medicine, which is applied in the pharmaceutical field of harpagoside, and can solve the problems that the effects of PD cell models and PD overall animal models have not yet been reported.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0083] Example 1: Harpagoside protection against MPTP + induced neuronal damage
[0084] Methods: The culture of midbrain dopamine neurons was the same as before. figure 1 A's experimental protocol.
[0085] Determination of cell viability: MTT method was used. The cultured cells were washed once with PBS, and MTT (0.5 mg / ml) was added to each well. After 4 hours of incubation, the supernatant was discarded, DMSO was added, and the cells were shaken on a shaker for 15 minutes to dissolve all, and the absorbance was read at 490 nm with a microplate reader. Cell viability = (without MPTP + Group OD-plus MPTP + Group OD) / (without MPTP + group OD-MPTP + Group OD) × 100% to obtain the change in the number of viable cells.
[0086] RESULTS: MPTP was observed experimentally in cell viability assays + Toxic effects on the damage of primary nerve cells, adding different concentrations of harpagoside (10 -6 M, 10 -5 M) The percentage of viable cells increased from 65.0% to 80....
Embodiment 2
[0091] Example 2: Harpagoside vs MPP + Protective effects of damaged midbrain dopamine neurons
[0092] Immunocytochemical staining of midbrain dopamine neurons for tyrosine hydroxylase (TH), the change in the number of TH positive (+) cells is a hallmark enzyme of PD. figure 2 A is to use figure 1The experimental protocol of A, the obtained picture. Observe under a Nikon inverted microscope, take 10 pictures in each culture well under a magnified field of 100 times, count the number of TH-positive neurons, and measure the longest protrusion of each TH-positive neuron in the field of view under a 200-fold field of view , for assessing TH-positive neuron neurite length.
[0093] figure 2 B shows an increase in the number of midbrain TH(+) neurons as the concentration of harpagoside increased from 0.1 μM to 10 μM. 1 μM and 10 μM harpagoside make MPP + The relative numbers of injured midbrain TH(+) neurons increased from (0.604±0.010) to (0.739±0.015) and (0.842±0.034), r...
Embodiment 3
[0096] Example 3: Harpagoside vs MPP + Repair (treatment) of damaged midbrain dopamine neurons
[0097] image 3 A is to use figure 1 Protocol B, immunocytochemical staining to obtain pictures of midbrain dopamine neurons. Counting the number and neurite lengths of TH-positive neurons revealed that 10 μM harpagoside in MPP + Added to the cell culture system 24 hours after injury, the number of midbrain TH(+) neurons ( image 3 B) and protrusion length ( image 3 C) showed an increasing trend. 10 μM and 100 μM harpagoside to make MPP + The relative number of injured midbrain TH(+) neurons increased from (0.699±0.020) to (0.781±0.021) and (0.824±0.018), an increase of 11.7% (P+ The number of damaged midbrain TH(+) neurons and the length of neurites were not significantly affected (both P>0.05).
[0098] The above results prove that the MPP + The injury was then treated with harpagoside, both 10 μM and 100 μM harpagoside could significantly repair (treat) MPP + Neuronal ...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More