Method for modifying bacterial cellulose in the fermentation process

A technology for bacterial cellulose and fermentation process, applied in the field of microbial fermentation, can solve the problems of irritation and toxicity of etherification reagents, high cost, complicated treatment process, etc., to expand the scope of application, reduce hydrogen bonding, and reduce reagent costs. Effect

Active Publication Date: 2011-10-05
海南椰国热带水果食品加工有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the process of this method is complicated, the cost of the reagents used is high, it is not conducive to large-scale industrial production, and the etherification reagents used are irritating and to

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for modifying bacterial cellulose during fermentation, comprising the following steps:

[0025] (1) Activating bacterial cellulose fermentation production strain Acetobacter xylinum, and preparing seed liquid for fermentation production: the seed liquid culture medium contains mannitol 25g / l, yeast extract 5g / l, peptone 3g / l, each 1 Put 500ml of liquid culture medium in a 1-liter shaker flask, and cultivate it for 24 hours at 180 rotations and 30 degrees to obtain a seed solution;

[0026] (2) The seed liquid is added in the fermentation medium according to the inoculation amount of 10% by volume, and the fermentation medium contains: acetic acid: 0.2%, sucrose: 10%, HPMC: 1%, Na-CMC: 1%, the rest for coconut water;

[0027] (3) 30°C, culture in a 250ml culture dish for 14 days;

[0028] (4) Collect the bacterial film formed on the surface of the culture medium, boil it in 0.5N NaOH for 10min, soak it in 0.5N NaOH at room temperature for 24h, rinse it with de...

Embodiment 2

[0034] A method for modifying bacterial cellulose during fermentation, comprising the following steps:

[0035] (1) Activating bacterial cellulose fermentation production strain Acetobacter xylinum, and preparing seed liquid for fermentation production: the seed liquid culture medium contains mannitol 25g / l, yeast extract 5g / l, peptone 3g / l, each 1 Put 500ml of liquid culture medium in a 1-liter shaker flask, and cultivate it for 24 hours at 180 rotations and 30 degrees to obtain a seed solution;

[0036] (2) The seed liquid is added in the fermentation medium according to the inoculum size of 8% by volume, and the fermentation medium contains acetic acid: 0.2%, glucose: 10%, HPMC: 5%, Na-CMC: 1%, and the rest is Coconut water;

[0037] (3) 30°C, culture in a 250ml culture dish for 16 days;

[0038] (4) Collect the bacterial film formed on the surface of the medium, boil it in 0.5N NaOH for 10min, soak it in 0.5N NaOH at room temperature for 24h, and rinse it with deionized ...

Embodiment 3

[0040] A method for modifying bacterial cellulose during fermentation, comprising the following steps:

[0041] (1) Activating bacterial cellulose fermentation production strain Acetobacter xylinum, and preparing seed liquid for fermentation production: the seed liquid culture medium contains mannitol 25g / l, yeast extract 5g / l, peptone 3g / l, each 1 Put 500ml of liquid culture medium in a 1-liter shaker flask, and cultivate it for 24 hours at 180 rotations and 30 degrees to obtain a seed solution;

[0042] (2) The seed liquid is added in the fermentation medium according to the inoculation amount of 12% by volume, and the fermentation medium contains acetic acid: 0.2%, sucrose: 5%, fructose: 5%, HPMC: 1% by weight, Na-CMC : 5%, the rest is coconut water;

[0043] (3) 30°C, culture in a 250ml culture dish for 12 days;

[0044] (4) collect the bacterium film that culture medium surface forms, boil 10min in 0.5N NaOH, soak 24h in 0.5N NaOH at room temperature, rinse to neutralit...

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PUM

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Abstract

The invention belongs to the field of microbial fermentation, and relates to a method for modifying bacterial cellulose in the fermentation process. The method comprises the following steps of: activating strains for fermentation production of the bacterial cellulose and preparing seed liquid; adding the seed liquid into a fermentation medium added with hydroxypropylmethylcellulose and carboxymethyl cellulose sodium, and standing for fermentation; and collecting a bacterial cellulose film generated on the surface of the medium, performing alkali washing, and drying until the water content is less than 5 percent. The method is simple and practicable; aftertreatment is not needed, the net structure of the bacterial cellulose can be changed directly in the bacterial cellulose fermentation process, and the hydrogen bond action in molecules and between molecules is reduced; moreover, used reagents are low in cost, and special equipment is not needed; and the prepared bacterial cellulose product has high rehydration and the application range of the bacterial cellulose product is widened.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and relates to a method for modifying bacterial cellulose in a fermentation process and bacterial cellulose prepared by the method. Background technique [0002] Bacterial cellulose (BC) refers to a kind of cellulose produced from Acetobacter, Agrobacterium, Rhizobium and Sarcina under different conditions. A general term for cellulose synthesized by microorganisms. Compared with natural cellulose, it has excellent properties such as high purity, high crystallinity, high degree of polymerization, and high water holding capacity, and has been widely used in various fields. [0003] The existing bacterial cellulose is produced and prepared in liquid culture medium, and the obtained bacterial cellulose gel film has a water content of more than 98%, a high water activity, and microorganisms are easy to breed and reproduce, and are not resistant to storage. High, bulky, inconvenient to transpor...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12R1/01C12R1/02
Inventor 钟春燕钟宇光
Owner 海南椰国热带水果食品加工有限公司
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